CD31 (PECAM-1) is a marker of recent thymic emigrants among CD4+ T-cells, but not CD8+ T-cells or ? d T-cells, in HIV patients responding to ART
Some severely immunodeficient HIV patients experience poor recovery of CD4 T-cell counts on antiretroviral therapy (ART). Evaluation of the function of thymopoiesis in T-cell production in individual patients requires a simple marker of T-cells that have recently emigrated from the thymus. Here, we...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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Nature Publishing Group
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/17404 |
| _version_ | 1848749457458331648 |
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| author | Tanaskovic, S. Fernandez, S. Price, Patricia Lee, S. French, M. |
| author_facet | Tanaskovic, S. Fernandez, S. Price, Patricia Lee, S. French, M. |
| author_sort | Tanaskovic, S. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Some severely immunodeficient HIV patients experience poor recovery of CD4 T-cell counts on antiretroviral therapy (ART). Evaluation of the function of thymopoiesis in T-cell production in individual patients requires a simple marker of T-cells that have recently emigrated from the thymus. Here, we address whether expression of CD31 on CD4 T-cells, CD8 T-cells, regulatory T-cells and ? T-cells correlates with other indicators of thymus function. Adult HIV-1 patients (n27) with nadir CD4 T-cell counts 100 per l and a sustained virological response to ART and healthy controls (n23) were studied. CD31 expression was assessed by flow cytometry, T-cell receptor excision circles content by real-time PCR and thymic volume by spiral computed tomography. Proportions of CD4 T-cells expressing CD45RA and CD31 declined with age in HIV patients (P0.03) and healthy controls (P0.0001), and correlated directly with other markers of thymus function. In controls, proportions of CD8 T-cells expressing CD45RA and CD31 declined with age (P0.003) and correlated directly with some markers of thymus function, but this was not seen in HIV patients. Proportions of CD45RA CD31 ? T-cells were higher in patients than controls (P0.007) and did not correlate with thymus volume. In controls, proportion of ? T-cells co-expressing CD45RA and CD31 increased with age (P0.002). These data support the use of CD31 as a marker of recent thymic origin in CD4 T-cells, but not CD8 T-cells in HIV patients receiving ART. In such patients, CD31 expression is unlikely to indicate thymic origin in ? T-cells. © 2010 Australasian Society for Immunology Inc. All rights reserved. |
| first_indexed | 2025-11-14T07:21:14Z |
| format | Journal Article |
| id | curtin-20.500.11937-17404 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:21:14Z |
| publishDate | 2010 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-174042017-09-13T15:42:22Z CD31 (PECAM-1) is a marker of recent thymic emigrants among CD4+ T-cells, but not CD8+ T-cells or ? d T-cells, in HIV patients responding to ART Tanaskovic, S. Fernandez, S. Price, Patricia Lee, S. French, M. Some severely immunodeficient HIV patients experience poor recovery of CD4 T-cell counts on antiretroviral therapy (ART). Evaluation of the function of thymopoiesis in T-cell production in individual patients requires a simple marker of T-cells that have recently emigrated from the thymus. Here, we address whether expression of CD31 on CD4 T-cells, CD8 T-cells, regulatory T-cells and ? T-cells correlates with other indicators of thymus function. Adult HIV-1 patients (n27) with nadir CD4 T-cell counts 100 per l and a sustained virological response to ART and healthy controls (n23) were studied. CD31 expression was assessed by flow cytometry, T-cell receptor excision circles content by real-time PCR and thymic volume by spiral computed tomography. Proportions of CD4 T-cells expressing CD45RA and CD31 declined with age in HIV patients (P0.03) and healthy controls (P0.0001), and correlated directly with other markers of thymus function. In controls, proportions of CD8 T-cells expressing CD45RA and CD31 declined with age (P0.003) and correlated directly with some markers of thymus function, but this was not seen in HIV patients. Proportions of CD45RA CD31 ? T-cells were higher in patients than controls (P0.007) and did not correlate with thymus volume. In controls, proportion of ? T-cells co-expressing CD45RA and CD31 increased with age (P0.002). These data support the use of CD31 as a marker of recent thymic origin in CD4 T-cells, but not CD8 T-cells in HIV patients receiving ART. In such patients, CD31 expression is unlikely to indicate thymic origin in ? T-cells. © 2010 Australasian Society for Immunology Inc. All rights reserved. 2010 Journal Article http://hdl.handle.net/20.500.11937/17404 10.1038/icb.2009.108 Nature Publishing Group unknown |
| spellingShingle | Tanaskovic, S. Fernandez, S. Price, Patricia Lee, S. French, M. CD31 (PECAM-1) is a marker of recent thymic emigrants among CD4+ T-cells, but not CD8+ T-cells or ? d T-cells, in HIV patients responding to ART |
| title | CD31 (PECAM-1) is a marker of recent thymic emigrants among CD4+ T-cells, but not CD8+ T-cells or ? d T-cells, in HIV patients responding to ART |
| title_full | CD31 (PECAM-1) is a marker of recent thymic emigrants among CD4+ T-cells, but not CD8+ T-cells or ? d T-cells, in HIV patients responding to ART |
| title_fullStr | CD31 (PECAM-1) is a marker of recent thymic emigrants among CD4+ T-cells, but not CD8+ T-cells or ? d T-cells, in HIV patients responding to ART |
| title_full_unstemmed | CD31 (PECAM-1) is a marker of recent thymic emigrants among CD4+ T-cells, but not CD8+ T-cells or ? d T-cells, in HIV patients responding to ART |
| title_short | CD31 (PECAM-1) is a marker of recent thymic emigrants among CD4+ T-cells, but not CD8+ T-cells or ? d T-cells, in HIV patients responding to ART |
| title_sort | cd31 (pecam-1) is a marker of recent thymic emigrants among cd4+ t-cells, but not cd8+ t-cells or ? d t-cells, in hiv patients responding to art |
| url | http://hdl.handle.net/20.500.11937/17404 |