Cyclic mechanical stimulation rescues achilles tendon from degeneration in a bioreactor system
Physiotherapy is one of the effective treatments for tendinopathy, whereby symptoms are relieved by changing the biomechanical environment of the pathological tendon. However, the underlying mechanism remains unclear. In this study, we first established a model of progressive tendinopathy-like degen...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
John Wiley and Sons Inc.
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/17055 |
| _version_ | 1848749353651404800 |
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| author | Wang, T. Lin, Z. Ni, M. Thien, C. Day, R. Gardiner, B. Rubenson, J. Kirk, Brett Smith, D. Wang, A. Lloyd, D. Wang, Y. Zheng, Q. Zheng, M. |
| author_facet | Wang, T. Lin, Z. Ni, M. Thien, C. Day, R. Gardiner, B. Rubenson, J. Kirk, Brett Smith, D. Wang, A. Lloyd, D. Wang, Y. Zheng, Q. Zheng, M. |
| author_sort | Wang, T. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Physiotherapy is one of the effective treatments for tendinopathy, whereby symptoms are relieved by changing the biomechanical environment of the pathological tendon. However, the underlying mechanism remains unclear. In this study, we first established a model of progressive tendinopathy-like degeneration in the rabbit Achilles. Following ex vivo loading deprivation culture in a bioreactor system for 6 and 12 days, tendons exhibited progressive degenerative changes, abnormal collagen type III production, increased cell apoptosis, and weakened mechanical properties. When intervention was applied at day 7 for another 6 days by using cyclic tensile mechanical stimulation (6% strain, 0.25Hz, 8h/day) in a bioreactor, the pathological changes and mechanical properties were almost restored to levels seen in healthy tendon. Our results indicated that a proper biomechanical environment was able to rescue early-stage pathological changes by increased collagen type I production, decreased collagen degradation and cell apoptosis. The ex vivo model developed in this study allows systematic study on the effect of mechanical stimulation on tendon biology. |
| first_indexed | 2025-11-14T07:19:35Z |
| format | Journal Article |
| id | curtin-20.500.11937-17055 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:19:35Z |
| publishDate | 2015 |
| publisher | John Wiley and Sons Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-170552017-09-13T15:43:54Z Cyclic mechanical stimulation rescues achilles tendon from degeneration in a bioreactor system Wang, T. Lin, Z. Ni, M. Thien, C. Day, R. Gardiner, B. Rubenson, J. Kirk, Brett Smith, D. Wang, A. Lloyd, D. Wang, Y. Zheng, Q. Zheng, M. Physiotherapy is one of the effective treatments for tendinopathy, whereby symptoms are relieved by changing the biomechanical environment of the pathological tendon. However, the underlying mechanism remains unclear. In this study, we first established a model of progressive tendinopathy-like degeneration in the rabbit Achilles. Following ex vivo loading deprivation culture in a bioreactor system for 6 and 12 days, tendons exhibited progressive degenerative changes, abnormal collagen type III production, increased cell apoptosis, and weakened mechanical properties. When intervention was applied at day 7 for another 6 days by using cyclic tensile mechanical stimulation (6% strain, 0.25Hz, 8h/day) in a bioreactor, the pathological changes and mechanical properties were almost restored to levels seen in healthy tendon. Our results indicated that a proper biomechanical environment was able to rescue early-stage pathological changes by increased collagen type I production, decreased collagen degradation and cell apoptosis. The ex vivo model developed in this study allows systematic study on the effect of mechanical stimulation on tendon biology. 2015 Journal Article http://hdl.handle.net/20.500.11937/17055 10.1002/jor.22960 John Wiley and Sons Inc. unknown |
| spellingShingle | Wang, T. Lin, Z. Ni, M. Thien, C. Day, R. Gardiner, B. Rubenson, J. Kirk, Brett Smith, D. Wang, A. Lloyd, D. Wang, Y. Zheng, Q. Zheng, M. Cyclic mechanical stimulation rescues achilles tendon from degeneration in a bioreactor system |
| title | Cyclic mechanical stimulation rescues achilles tendon from degeneration in a bioreactor system |
| title_full | Cyclic mechanical stimulation rescues achilles tendon from degeneration in a bioreactor system |
| title_fullStr | Cyclic mechanical stimulation rescues achilles tendon from degeneration in a bioreactor system |
| title_full_unstemmed | Cyclic mechanical stimulation rescues achilles tendon from degeneration in a bioreactor system |
| title_short | Cyclic mechanical stimulation rescues achilles tendon from degeneration in a bioreactor system |
| title_sort | cyclic mechanical stimulation rescues achilles tendon from degeneration in a bioreactor system |
| url | http://hdl.handle.net/20.500.11937/17055 |