Respiratory Pathogens Adopt a Chronic Lifestyle in Response to Bile

Chronic respiratory infections are a major cause of morbidity and mortality, most particularly in Cystic Fibrosis (CF) patients. The recent finding that gastro-esophageal reflux (GER) frequently occurs in CF patients led us to investigate the impact of bile on the behaviour of Pseudomonas aeruginosa...

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Main Authors: Reen, F., Woods, D., Mooij, M., Adams, C., O'Gara, Fergal
Format: Journal Article
Published: PLOS 2012
Online Access:http://hdl.handle.net/20.500.11937/16850
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author Reen, F.
Woods, D.
Mooij, M.
Adams, C.
O'Gara, Fergal
author_facet Reen, F.
Woods, D.
Mooij, M.
Adams, C.
O'Gara, Fergal
author_sort Reen, F.
building Curtin Institutional Repository
collection Online Access
description Chronic respiratory infections are a major cause of morbidity and mortality, most particularly in Cystic Fibrosis (CF) patients. The recent finding that gastro-esophageal reflux (GER) frequently occurs in CF patients led us to investigate the impact of bile on the behaviour of Pseudomonas aeruginosa and other CF-associated respiratory pathogens. Bile increased biofilm formation, Type Six Secretion, and quorum sensing in P. aeruginosa, all of which are associated with the switch from acute to persistent infection. Furthermore, bile negatively influenced Type Three Secretion and swarming motility in P. aeruginosa, phenotypes associated with acute infection. Bile also modulated biofilm formation in a range of other CF-associated respiratory pathogens, including Burkholderia cepacia and Staphylococcus aureus. Therefore, our results suggest that GER-derived bile may be a host determinant contributing to chronic respiratory infection.
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spelling curtin-20.500.11937-168502017-09-13T15:43:29Z Respiratory Pathogens Adopt a Chronic Lifestyle in Response to Bile Reen, F. Woods, D. Mooij, M. Adams, C. O'Gara, Fergal Chronic respiratory infections are a major cause of morbidity and mortality, most particularly in Cystic Fibrosis (CF) patients. The recent finding that gastro-esophageal reflux (GER) frequently occurs in CF patients led us to investigate the impact of bile on the behaviour of Pseudomonas aeruginosa and other CF-associated respiratory pathogens. Bile increased biofilm formation, Type Six Secretion, and quorum sensing in P. aeruginosa, all of which are associated with the switch from acute to persistent infection. Furthermore, bile negatively influenced Type Three Secretion and swarming motility in P. aeruginosa, phenotypes associated with acute infection. Bile also modulated biofilm formation in a range of other CF-associated respiratory pathogens, including Burkholderia cepacia and Staphylococcus aureus. Therefore, our results suggest that GER-derived bile may be a host determinant contributing to chronic respiratory infection. 2012 Journal Article http://hdl.handle.net/20.500.11937/16850 10.1371/journal.pone.0045978 PLOS unknown
spellingShingle Reen, F.
Woods, D.
Mooij, M.
Adams, C.
O'Gara, Fergal
Respiratory Pathogens Adopt a Chronic Lifestyle in Response to Bile
title Respiratory Pathogens Adopt a Chronic Lifestyle in Response to Bile
title_full Respiratory Pathogens Adopt a Chronic Lifestyle in Response to Bile
title_fullStr Respiratory Pathogens Adopt a Chronic Lifestyle in Response to Bile
title_full_unstemmed Respiratory Pathogens Adopt a Chronic Lifestyle in Response to Bile
title_short Respiratory Pathogens Adopt a Chronic Lifestyle in Response to Bile
title_sort respiratory pathogens adopt a chronic lifestyle in response to bile
url http://hdl.handle.net/20.500.11937/16850