Dynamics Analysis and Prediction of Genetic Regulation in Glycerol Metabolic Network via Structural Kinetic Modelling
Glycerol can be biologically converted to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae. In the synthesis pathway of 1,3-PD, the accumulation of an intermediary metabolite 3-hydroxypropionaldehyde (3-HPA) would cause an irreversible cessation of the dynamic system. Genetic manipulation on the ke...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
Gordon and Breach
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/16719 |
| _version_ | 1848749257167732736 |
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| author | Ye, J. Xu, Honglei Huang, X. Ke, C. Feng, E. |
| author_facet | Ye, J. Xu, Honglei Huang, X. Ke, C. Feng, E. |
| author_sort | Ye, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Glycerol can be biologically converted to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae. In the synthesis pathway of 1,3-PD, the accumulation of an intermediary metabolite 3-hydroxypropionaldehyde (3-HPA) would cause an irreversible cessation of the dynamic system. Genetic manipulation on the key enzymes which control the formation rate and consumption rate of 3-HPA would decrease the accumulation of 3-HPA, resulting in nonlinear regulation on the dynamic system. The interest of this work is to focus on analyzing the influence of 3-HPA inhibition on the stability of the dynamic system. Due to the lack of intracellular knowledge, structural kinetic modelling is applied. On the basis of statistical account of the dynamical capabilities of the system inthe parameter space,we conclude that, underweak or no inhibition to the reaction of 3-HPAconsumption, the systemismuch easier to obtain a stable state, whereas strong inhibition to its formation is in favor of stabilizing the system. In addition, the existence of Hopf bifurcation in this systemis also verified. The obtained results are helpful for deeply understanding the metabolic and genetic regulations of glycerol fermentation by Klebsiella pneumoniae. |
| first_indexed | 2025-11-14T07:18:03Z |
| format | Journal Article |
| id | curtin-20.500.11937-16719 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:18:03Z |
| publishDate | 2015 |
| publisher | Gordon and Breach |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-167192017-09-13T15:44:15Z Dynamics Analysis and Prediction of Genetic Regulation in Glycerol Metabolic Network via Structural Kinetic Modelling Ye, J. Xu, Honglei Huang, X. Ke, C. Feng, E. Glycerol can be biologically converted to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae. In the synthesis pathway of 1,3-PD, the accumulation of an intermediary metabolite 3-hydroxypropionaldehyde (3-HPA) would cause an irreversible cessation of the dynamic system. Genetic manipulation on the key enzymes which control the formation rate and consumption rate of 3-HPA would decrease the accumulation of 3-HPA, resulting in nonlinear regulation on the dynamic system. The interest of this work is to focus on analyzing the influence of 3-HPA inhibition on the stability of the dynamic system. Due to the lack of intracellular knowledge, structural kinetic modelling is applied. On the basis of statistical account of the dynamical capabilities of the system inthe parameter space,we conclude that, underweak or no inhibition to the reaction of 3-HPAconsumption, the systemismuch easier to obtain a stable state, whereas strong inhibition to its formation is in favor of stabilizing the system. In addition, the existence of Hopf bifurcation in this systemis also verified. The obtained results are helpful for deeply understanding the metabolic and genetic regulations of glycerol fermentation by Klebsiella pneumoniae. 2015 Journal Article http://hdl.handle.net/20.500.11937/16719 10.1155/2015/673120 Gordon and Breach fulltext |
| spellingShingle | Ye, J. Xu, Honglei Huang, X. Ke, C. Feng, E. Dynamics Analysis and Prediction of Genetic Regulation in Glycerol Metabolic Network via Structural Kinetic Modelling |
| title | Dynamics Analysis and Prediction of Genetic Regulation in Glycerol Metabolic Network via Structural Kinetic Modelling |
| title_full | Dynamics Analysis and Prediction of Genetic Regulation in Glycerol Metabolic Network via Structural Kinetic Modelling |
| title_fullStr | Dynamics Analysis and Prediction of Genetic Regulation in Glycerol Metabolic Network via Structural Kinetic Modelling |
| title_full_unstemmed | Dynamics Analysis and Prediction of Genetic Regulation in Glycerol Metabolic Network via Structural Kinetic Modelling |
| title_short | Dynamics Analysis and Prediction of Genetic Regulation in Glycerol Metabolic Network via Structural Kinetic Modelling |
| title_sort | dynamics analysis and prediction of genetic regulation in glycerol metabolic network via structural kinetic modelling |
| url | http://hdl.handle.net/20.500.11937/16719 |