Sarcostemma viminale: a potential anticancer therapy
There is a need for cancer treatments to be selectively cytotoxic to cancer cells so as to reduce adverse side effects. In this study, a cancer cell line (HeLa cells) and a non-cancer cell line (HF-32) were exposed to an extract from the plant Sarcostemma viminale. Cytopathic effects and apoptosis w...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
Springer U K
2013
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/16552 |
| _version_ | 1848749208902828032 |
|---|---|
| author | Brestovac, Brian Townsend, D. Snook, J. Ellison, Gaewyn Phillips, A. |
| author_facet | Brestovac, Brian Townsend, D. Snook, J. Ellison, Gaewyn Phillips, A. |
| author_sort | Brestovac, Brian |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | There is a need for cancer treatments to be selectively cytotoxic to cancer cells so as to reduce adverse side effects. In this study, a cancer cell line (HeLa cells) and a non-cancer cell line (HF-32) were exposed to an extract from the plant Sarcostemma viminale. Cytopathic effects and apoptosis were measured by morphological changes, annexin V/propidium iodide (PI) and 4′,6-diamidino-2-phenylindole (DAPI) staining assays. Also, a novel mixed culture flow cytometry assay was performed exploiting the overexpression of p16INK4a in HeLa cells to demonstrate the change in numbers of HeLa and HF-32 cells post-exposure to the extract. At 1 % (v/v) after 48 h of exposure, HeLa cells showed >75 % cytopathic effect, 77 % were in apoptosis or dead by the annexinV/PI assay, and 100 % had nuclear changes by DAPI staining; there was a reduction of 76 % in the number of cells by mixed culture assay. In contrast, for the HF-32 cells, only 5 % showed any cytopathic effect, there were no more cells in apoptosis or dead (34 %) than in the control by the annexinV/PI assay, <1 % of cells had nuclear changes by DAPI staining, and there was a slight increase in cell numbers by the mixed culture assay. Results from these assays clearly demonstrate that the extract from S. viminale destroyed the cancer HeLa cells quickly and at a low concentration, whilst the non-cancer HF-32 cells survive. This study indicates that extracts from S. viminale may be a specific anticancer agent. |
| first_indexed | 2025-11-14T07:17:17Z |
| format | Journal Article |
| id | curtin-20.500.11937-16552 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:17:17Z |
| publishDate | 2013 |
| publisher | Springer U K |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-165522017-09-13T15:42:21Z Sarcostemma viminale: a potential anticancer therapy Brestovac, Brian Townsend, D. Snook, J. Ellison, Gaewyn Phillips, A. Apoptosis Mixed culture Anticancer Cytopathic effect Sarcostemma viminale There is a need for cancer treatments to be selectively cytotoxic to cancer cells so as to reduce adverse side effects. In this study, a cancer cell line (HeLa cells) and a non-cancer cell line (HF-32) were exposed to an extract from the plant Sarcostemma viminale. Cytopathic effects and apoptosis were measured by morphological changes, annexin V/propidium iodide (PI) and 4′,6-diamidino-2-phenylindole (DAPI) staining assays. Also, a novel mixed culture flow cytometry assay was performed exploiting the overexpression of p16INK4a in HeLa cells to demonstrate the change in numbers of HeLa and HF-32 cells post-exposure to the extract. At 1 % (v/v) after 48 h of exposure, HeLa cells showed >75 % cytopathic effect, 77 % were in apoptosis or dead by the annexinV/PI assay, and 100 % had nuclear changes by DAPI staining; there was a reduction of 76 % in the number of cells by mixed culture assay. In contrast, for the HF-32 cells, only 5 % showed any cytopathic effect, there were no more cells in apoptosis or dead (34 %) than in the control by the annexinV/PI assay, <1 % of cells had nuclear changes by DAPI staining, and there was a slight increase in cell numbers by the mixed culture assay. Results from these assays clearly demonstrate that the extract from S. viminale destroyed the cancer HeLa cells quickly and at a low concentration, whilst the non-cancer HF-32 cells survive. This study indicates that extracts from S. viminale may be a specific anticancer agent. 2013 Journal Article http://hdl.handle.net/20.500.11937/16552 10.1007/s00580-013-1843-0 Springer U K restricted |
| spellingShingle | Apoptosis Mixed culture Anticancer Cytopathic effect Sarcostemma viminale Brestovac, Brian Townsend, D. Snook, J. Ellison, Gaewyn Phillips, A. Sarcostemma viminale: a potential anticancer therapy |
| title | Sarcostemma viminale: a potential anticancer therapy |
| title_full | Sarcostemma viminale: a potential anticancer therapy |
| title_fullStr | Sarcostemma viminale: a potential anticancer therapy |
| title_full_unstemmed | Sarcostemma viminale: a potential anticancer therapy |
| title_short | Sarcostemma viminale: a potential anticancer therapy |
| title_sort | sarcostemma viminale: a potential anticancer therapy |
| topic | Apoptosis Mixed culture Anticancer Cytopathic effect Sarcostemma viminale |
| url | http://hdl.handle.net/20.500.11937/16552 |