Immobilization of halophilic Aspergillus awamori EM66 exochitinase on grafted k-carrageenan-alginate beads
A novel extreme halophilic exochitinase enzyme was produced by honey isolate Aspergillus awamori EM66. The enzyme was immobilized successfully on k-carrageenan-alginate gel carrier (CA) with 93 % immobilization yield. The immobilization process significantly improved the enzyme specific activity 2.6...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Published: |
Springer
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/15706 |
| _version_ | 1848748966877855744 |
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| author | Esawy, M. Awad, G. Wahab, W. Elnashar, Magdy El-Diwany, A. Easa, S. El-beih, F. |
| author_facet | Esawy, M. Awad, G. Wahab, W. Elnashar, Magdy El-Diwany, A. Easa, S. El-beih, F. |
| author_sort | Esawy, M. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | A novel extreme halophilic exochitinase enzyme was produced by honey isolate Aspergillus awamori EM66. The enzyme was immobilized successfully on k-carrageenan-alginate gel carrier (CA) with 93 % immobilization yield. The immobilization process significantly improved the enzyme specific activity 2.6-fold compared to the free form. The significant factors influencing the immobilization process such as enzyme protein concentration and loading time were studied. Distinguishable characteristics of optimum pH and temperature, stability at different temperatures and NaCl tolerance for free and immobilized enzyme were studied. The immobilization process improved optimum temperature from 35 to 45 °C. The immobilized enzyme retained 76.70 % of its activity after 2 h at 75 °C compared to complete loss of activity for the free enzyme. The reusability test proved the durability of the CA gel beads for 28 cycles without losing its activity. |
| first_indexed | 2025-11-14T07:13:27Z |
| format | Journal Article |
| id | curtin-20.500.11937-15706 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:13:27Z |
| publishDate | 2016 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-157062017-09-13T14:07:14Z Immobilization of halophilic Aspergillus awamori EM66 exochitinase on grafted k-carrageenan-alginate beads Esawy, M. Awad, G. Wahab, W. Elnashar, Magdy El-Diwany, A. Easa, S. El-beih, F. A novel extreme halophilic exochitinase enzyme was produced by honey isolate Aspergillus awamori EM66. The enzyme was immobilized successfully on k-carrageenan-alginate gel carrier (CA) with 93 % immobilization yield. The immobilization process significantly improved the enzyme specific activity 2.6-fold compared to the free form. The significant factors influencing the immobilization process such as enzyme protein concentration and loading time were studied. Distinguishable characteristics of optimum pH and temperature, stability at different temperatures and NaCl tolerance for free and immobilized enzyme were studied. The immobilization process improved optimum temperature from 35 to 45 °C. The immobilized enzyme retained 76.70 % of its activity after 2 h at 75 °C compared to complete loss of activity for the free enzyme. The reusability test proved the durability of the CA gel beads for 28 cycles without losing its activity. 2016 Journal Article http://hdl.handle.net/20.500.11937/15706 10.1007/s13205-015-0333-2 Springer fulltext |
| spellingShingle | Esawy, M. Awad, G. Wahab, W. Elnashar, Magdy El-Diwany, A. Easa, S. El-beih, F. Immobilization of halophilic Aspergillus awamori EM66 exochitinase on grafted k-carrageenan-alginate beads |
| title | Immobilization of halophilic Aspergillus awamori EM66 exochitinase on grafted k-carrageenan-alginate beads |
| title_full | Immobilization of halophilic Aspergillus awamori EM66 exochitinase on grafted k-carrageenan-alginate beads |
| title_fullStr | Immobilization of halophilic Aspergillus awamori EM66 exochitinase on grafted k-carrageenan-alginate beads |
| title_full_unstemmed | Immobilization of halophilic Aspergillus awamori EM66 exochitinase on grafted k-carrageenan-alginate beads |
| title_short | Immobilization of halophilic Aspergillus awamori EM66 exochitinase on grafted k-carrageenan-alginate beads |
| title_sort | immobilization of halophilic aspergillus awamori em66 exochitinase on grafted k-carrageenan-alginate beads |
| url | http://hdl.handle.net/20.500.11937/15706 |