Diuretic activity and toxicity of some Verbascum nigrum extracts and fractions
CONTEXT: Verbascum nigrum L. (Scrophulariaceae) is a perennial plant used in folk medicine for the treatment of kidney diseases due to its presumable diuretic properties. OBJECTIVE: We investigated the diuretic activity and toxicity of extracts from different parts of V. nigrum and identified a g...
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| Format: | Journal Article |
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Swets and Zeitlinger
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/15660 |
| _version_ | 1848748954001342464 |
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| author | Kalinina, Svetlana Elkina, Olga Kalinin, Dmitrii Syropyatov, Boris Dolzhenko, Anton |
| author_facet | Kalinina, Svetlana Elkina, Olga Kalinin, Dmitrii Syropyatov, Boris Dolzhenko, Anton |
| author_sort | Kalinina, Svetlana |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | CONTEXT: Verbascum nigrum L. (Scrophulariaceae) is a perennial plant used in folk medicine for the treatment of kidney diseases due to its presumable diuretic properties. OBJECTIVE: We investigated the diuretic activity and toxicity of extracts from different parts of V. nigrum and identified a group of compounds responsible for the biological effect. MATERIALS AND METHODS: Five ethanol extracts from herb, roots, flowers, leaves and stems as well as five fractions of polar compounds isolated from herb of V. nigrum were orally administrated as a single dose of 50 mg/kg to rats. Urinary excretion and electrolyte content were measured at 3 and 6 h after the treatment. The acute toxicity of the V. nigrum extracts and fractions was evaluated in mice.RESULTS: All extracts, except the one prepared from the roots, showed a significant increase of the urine output within first 3 h after their administration. The extract from stems was the most active, inducing urine output of 14.6 ± 0.8 ml/kg BW versus 5.2 ± 1.4 ml/kg BW of the control. It also demonstrated saluretic activity with a natriuretic index 4.1 and a kaliuretic index 3.8. The diuretic activity was correlated with the flavonoid content in the plant organs. Flavonoid fractions demonstrated significant activity; the higher content of flavonoids (expressed as hesperidin) translated into more pronounced diuretic (35.9 ± 2.1 ml/kg BW) and saluretic effects (natriuretic index 4.5 and kaliuretic index 5.4). CONCLUSION: The diuretic activity of traditionally used V. nigrum was validated experimentally. The pharmacological effect was attributed to flavonoids, which accumulated in aerial parts of the plant, mainly in stems. |
| first_indexed | 2025-11-14T07:13:14Z |
| format | Journal Article |
| id | curtin-20.500.11937-15660 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:13:14Z |
| publishDate | 2014 |
| publisher | Swets and Zeitlinger |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-156602017-09-13T16:00:10Z Diuretic activity and toxicity of some Verbascum nigrum extracts and fractions Kalinina, Svetlana Elkina, Olga Kalinin, Dmitrii Syropyatov, Boris Dolzhenko, Anton Scrophulariaceae electrolyte excretion Diuresis mullein hesperidin flavonoids CONTEXT: Verbascum nigrum L. (Scrophulariaceae) is a perennial plant used in folk medicine for the treatment of kidney diseases due to its presumable diuretic properties. OBJECTIVE: We investigated the diuretic activity and toxicity of extracts from different parts of V. nigrum and identified a group of compounds responsible for the biological effect. MATERIALS AND METHODS: Five ethanol extracts from herb, roots, flowers, leaves and stems as well as five fractions of polar compounds isolated from herb of V. nigrum were orally administrated as a single dose of 50 mg/kg to rats. Urinary excretion and electrolyte content were measured at 3 and 6 h after the treatment. The acute toxicity of the V. nigrum extracts and fractions was evaluated in mice.RESULTS: All extracts, except the one prepared from the roots, showed a significant increase of the urine output within first 3 h after their administration. The extract from stems was the most active, inducing urine output of 14.6 ± 0.8 ml/kg BW versus 5.2 ± 1.4 ml/kg BW of the control. It also demonstrated saluretic activity with a natriuretic index 4.1 and a kaliuretic index 3.8. The diuretic activity was correlated with the flavonoid content in the plant organs. Flavonoid fractions demonstrated significant activity; the higher content of flavonoids (expressed as hesperidin) translated into more pronounced diuretic (35.9 ± 2.1 ml/kg BW) and saluretic effects (natriuretic index 4.5 and kaliuretic index 5.4). CONCLUSION: The diuretic activity of traditionally used V. nigrum was validated experimentally. The pharmacological effect was attributed to flavonoids, which accumulated in aerial parts of the plant, mainly in stems. 2014 Journal Article http://hdl.handle.net/20.500.11937/15660 10.3109/13880209.2013.822001 Swets and Zeitlinger unknown |
| spellingShingle | Scrophulariaceae electrolyte excretion Diuresis mullein hesperidin flavonoids Kalinina, Svetlana Elkina, Olga Kalinin, Dmitrii Syropyatov, Boris Dolzhenko, Anton Diuretic activity and toxicity of some Verbascum nigrum extracts and fractions |
| title | Diuretic activity and toxicity of some Verbascum nigrum extracts and fractions |
| title_full | Diuretic activity and toxicity of some Verbascum nigrum extracts and fractions |
| title_fullStr | Diuretic activity and toxicity of some Verbascum nigrum extracts and fractions |
| title_full_unstemmed | Diuretic activity and toxicity of some Verbascum nigrum extracts and fractions |
| title_short | Diuretic activity and toxicity of some Verbascum nigrum extracts and fractions |
| title_sort | diuretic activity and toxicity of some verbascum nigrum extracts and fractions |
| topic | Scrophulariaceae electrolyte excretion Diuresis mullein hesperidin flavonoids |
| url | http://hdl.handle.net/20.500.11937/15660 |