Biocompatible and mucoadhesive bacterial cellulose-g-poly(acrylic acid) hydrogels for oral protein delivery.
Stimuli-responsive bacterial cellulose-g-poly-(acrylic acid) hydrogels were investigated for their potential use as an oral delivery system for proteins. These hydrogels were synthesized using electron beam irradiation without any cross-linking agents, thereby eliminating any potential toxic effects...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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American Chemical Society
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/15649 |
| _version_ | 1848748951143972864 |
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| author | Ahmad, N. Mohd Amin, M.C. Mohd Mahali, S. Ismail, I. Chuang, Victor |
| author_facet | Ahmad, N. Mohd Amin, M.C. Mohd Mahali, S. Ismail, I. Chuang, Victor |
| author_sort | Ahmad, N. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Stimuli-responsive bacterial cellulose-g-poly-(acrylic acid) hydrogels were investigated for their potential use as an oral delivery system for proteins. These hydrogels were synthesized using electron beam irradiation without any cross-linking agents, thereby eliminating any potential toxic effects associated with cross-linkers. Bovine serum albumin (BSA), a model protein drug, was loaded into the hydrogels, and the release profile in simulated gastrointestinal fluids was investigated. Cumulative release of less than 10% in simulated gastric fluid (SGF) demonstrated the potential of these hydrogels to protect BSA from the acidic environment of the stomach. Subsequent conformational stability analyses of released BSA by SDS-PAGE, circular dichroism, and an esterase activity assay indicated that the structural integrity and bioactivity of BSA was maintained and preserved by the hydrogels. Furthermore, an increase in BSA penetration across intestinal mucosa tissue was observed in an ex vivo penetration experiment. Our fabricated hydrogels exhibited excellent cytocompatibility and showed no sign of toxicity, indicating the safety of these hydrogels for in vivo applications. |
| first_indexed | 2025-11-14T07:13:12Z |
| format | Journal Article |
| id | curtin-20.500.11937-15649 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:13:12Z |
| publishDate | 2014 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-156492017-09-13T13:41:44Z Biocompatible and mucoadhesive bacterial cellulose-g-poly(acrylic acid) hydrogels for oral protein delivery. Ahmad, N. Mohd Amin, M.C. Mohd Mahali, S. Ismail, I. Chuang, Victor protein conformational stability bacterial cellulose hydrogels oral protein delivery electron beam biocompatibility Stimuli-responsive bacterial cellulose-g-poly-(acrylic acid) hydrogels were investigated for their potential use as an oral delivery system for proteins. These hydrogels were synthesized using electron beam irradiation without any cross-linking agents, thereby eliminating any potential toxic effects associated with cross-linkers. Bovine serum albumin (BSA), a model protein drug, was loaded into the hydrogels, and the release profile in simulated gastrointestinal fluids was investigated. Cumulative release of less than 10% in simulated gastric fluid (SGF) demonstrated the potential of these hydrogels to protect BSA from the acidic environment of the stomach. Subsequent conformational stability analyses of released BSA by SDS-PAGE, circular dichroism, and an esterase activity assay indicated that the structural integrity and bioactivity of BSA was maintained and preserved by the hydrogels. Furthermore, an increase in BSA penetration across intestinal mucosa tissue was observed in an ex vivo penetration experiment. Our fabricated hydrogels exhibited excellent cytocompatibility and showed no sign of toxicity, indicating the safety of these hydrogels for in vivo applications. 2014 Journal Article http://hdl.handle.net/20.500.11937/15649 10.1021/mp5003015 American Chemical Society restricted |
| spellingShingle | protein conformational stability bacterial cellulose hydrogels oral protein delivery electron beam biocompatibility Ahmad, N. Mohd Amin, M.C. Mohd Mahali, S. Ismail, I. Chuang, Victor Biocompatible and mucoadhesive bacterial cellulose-g-poly(acrylic acid) hydrogels for oral protein delivery. |
| title | Biocompatible and mucoadhesive bacterial cellulose-g-poly(acrylic acid) hydrogels for oral protein delivery. |
| title_full | Biocompatible and mucoadhesive bacterial cellulose-g-poly(acrylic acid) hydrogels for oral protein delivery. |
| title_fullStr | Biocompatible and mucoadhesive bacterial cellulose-g-poly(acrylic acid) hydrogels for oral protein delivery. |
| title_full_unstemmed | Biocompatible and mucoadhesive bacterial cellulose-g-poly(acrylic acid) hydrogels for oral protein delivery. |
| title_short | Biocompatible and mucoadhesive bacterial cellulose-g-poly(acrylic acid) hydrogels for oral protein delivery. |
| title_sort | biocompatible and mucoadhesive bacterial cellulose-g-poly(acrylic acid) hydrogels for oral protein delivery. |
| topic | protein conformational stability bacterial cellulose hydrogels oral protein delivery electron beam biocompatibility |
| url | http://hdl.handle.net/20.500.11937/15649 |