hShroom1 links a membrane bound protein to the actin cytoskeleton
hShroom1 (hShrm1) is a member of the Apx/Shroom (Shrm) protein family and was identified from a yeast two-hybrid screen as a protein that interacts with the cytoplasmic domain of melanoma cell adhesion molecule (MCAM). The characteristic signature of the Shrm family is the presence of a unique domai...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
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Birkhauser Verlag
2009
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| Online Access: | http://hdl.handle.net/20.500.11937/15471 |
| _version_ | 1848748902481657856 |
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| author | Dye, Danielle Karlen, S. Rohrbach, B. Staub, O. Braathen, L. Eidne, K. Coombe, Deirdre |
| author_facet | Dye, Danielle Karlen, S. Rohrbach, B. Staub, O. Braathen, L. Eidne, K. Coombe, Deirdre |
| author_sort | Dye, Danielle |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | hShroom1 (hShrm1) is a member of the Apx/Shroom (Shrm) protein family and was identified from a yeast two-hybrid screen as a protein that interacts with the cytoplasmic domain of melanoma cell adhesion molecule (MCAM). The characteristic signature of the Shrm family is the presence of a unique domain, ASD2 (Apx/Shroom domain 2). mRNA analysis suggests that hShrm1 is expressed in brain, heart, skeletal muscle, colon, small intestine, kidney, placenta and lung tissue, as well a variety of melanoma and other cell lines. Co-immunoprecipitation and bioluminescence resonance energy transfer (BRET) experiments indicate that hShrm1 and MCAM interact in vivo and by immunofluorescence microscopy some co-localization of these proteins is observed. hShrm1 partly co-localises with β-actin and is found in the Triton X-100 insoluble fraction of melanoma cell extracts. We propose that hShrm1 is involved in linking MCAM to the cytoskeleton. |
| first_indexed | 2025-11-14T07:12:25Z |
| format | Journal Article |
| id | curtin-20.500.11937-15471 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:12:25Z |
| publishDate | 2009 |
| publisher | Birkhauser Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-154712017-09-13T16:05:07Z hShroom1 links a membrane bound protein to the actin cytoskeleton Dye, Danielle Karlen, S. Rohrbach, B. Staub, O. Braathen, L. Eidne, K. Coombe, Deirdre Shroom cytoskeleton MCAM Melanoma hShroom1 (hShrm1) is a member of the Apx/Shroom (Shrm) protein family and was identified from a yeast two-hybrid screen as a protein that interacts with the cytoplasmic domain of melanoma cell adhesion molecule (MCAM). The characteristic signature of the Shrm family is the presence of a unique domain, ASD2 (Apx/Shroom domain 2). mRNA analysis suggests that hShrm1 is expressed in brain, heart, skeletal muscle, colon, small intestine, kidney, placenta and lung tissue, as well a variety of melanoma and other cell lines. Co-immunoprecipitation and bioluminescence resonance energy transfer (BRET) experiments indicate that hShrm1 and MCAM interact in vivo and by immunofluorescence microscopy some co-localization of these proteins is observed. hShrm1 partly co-localises with β-actin and is found in the Triton X-100 insoluble fraction of melanoma cell extracts. We propose that hShrm1 is involved in linking MCAM to the cytoskeleton. 2009 Journal Article http://hdl.handle.net/20.500.11937/15471 10.1007/s00018-009-8645-1 Birkhauser Verlag restricted |
| spellingShingle | Shroom cytoskeleton MCAM Melanoma Dye, Danielle Karlen, S. Rohrbach, B. Staub, O. Braathen, L. Eidne, K. Coombe, Deirdre hShroom1 links a membrane bound protein to the actin cytoskeleton |
| title | hShroom1 links a membrane bound protein to the actin cytoskeleton |
| title_full | hShroom1 links a membrane bound protein to the actin cytoskeleton |
| title_fullStr | hShroom1 links a membrane bound protein to the actin cytoskeleton |
| title_full_unstemmed | hShroom1 links a membrane bound protein to the actin cytoskeleton |
| title_short | hShroom1 links a membrane bound protein to the actin cytoskeleton |
| title_sort | hshroom1 links a membrane bound protein to the actin cytoskeleton |
| topic | Shroom cytoskeleton MCAM Melanoma |
| url | http://hdl.handle.net/20.500.11937/15471 |