Biological Assessments of Encapsulated Pancreatic ß-Cells: Their Potential Transplantation in Diabetes
© 2016. Biomedical Engineering Society. Microencapsulation of pancreatic islets has been considered as a promising method for cell transplantation and diabetes treatment. However, in vivo trials to date have been hampered by fibrotic overgrowth and very limited to no success, long-term. Future succe...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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Springer New York LLC
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/15066 |
| _version_ | 1848748794424852480 |
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| author | Mooranian, Armin Negrulj, Rebecca Jamieson, E. Morahan, G. Al-Salami, Hani |
| author_facet | Mooranian, Armin Negrulj, Rebecca Jamieson, E. Morahan, G. Al-Salami, Hani |
| author_sort | Mooranian, Armin |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2016. Biomedical Engineering Society. Microencapsulation of pancreatic islets has been considered as a promising method for cell transplantation and diabetes treatment. However, in vivo trials to date have been hampered by fibrotic overgrowth and very limited to no success, long-term. Future success requires suitable microencapsulating method and possibly a simplified and suitable formulation which will produce a microcapsule that provides an immunobarrier, maintain full ß-cell functionality whilst also reducing the inflammatory processes that induce fibrosis. In multiple studies, we screened various formulations and microencapsulating methods, and obtained promising results using bile acid-based microcapsules containing ß-cells, in terms of cell functions and insulin release. Thus, this study aimed to refine further the microencapsulating method using a simple alginate-poly-l-ornithine formulation and test the effect of adding a promising bile acid, ursodeoxycholic acid (UDCA), on cell functions. Using Büchi concentric nozzle, viable NIT-1 cells were microencapsulated using alginate-poly-l-ornithine, with or without UDCA at a ratio of 1:1.2 or 1:1.2:4. Screening for nozzle temperature and nozzle-gelation bath distance was carried out to form best microcapsules. Microcapsules were cultured for 48 h and examined for size and surface morphology, chemical profiling and ß-cell viability. Culture supernatants were examined for insulin and inflammatory cytokines. When using 30 °C nozzle-temperature and 5 cm nozzle-gelation bath distance, in the presence of the bile acid, cell mitochondrial activities and insulin production were optimised. Under deployed microencapsulating method with nozzle-temperature of 30 °C and nozzle-gelation bath distance of 5 cm, the incorporation of the bile acid into the microcapsules resulted in enhanced ß-cell survival, function and improved overall biocompatibility supporting potential applications in transplantation. |
| first_indexed | 2025-11-14T07:10:42Z |
| format | Journal Article |
| id | curtin-20.500.11937-15066 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:10:42Z |
| publishDate | 2016 |
| publisher | Springer New York LLC |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-150662017-09-13T15:04:05Z Biological Assessments of Encapsulated Pancreatic ß-Cells: Their Potential Transplantation in Diabetes Mooranian, Armin Negrulj, Rebecca Jamieson, E. Morahan, G. Al-Salami, Hani © 2016. Biomedical Engineering Society. Microencapsulation of pancreatic islets has been considered as a promising method for cell transplantation and diabetes treatment. However, in vivo trials to date have been hampered by fibrotic overgrowth and very limited to no success, long-term. Future success requires suitable microencapsulating method and possibly a simplified and suitable formulation which will produce a microcapsule that provides an immunobarrier, maintain full ß-cell functionality whilst also reducing the inflammatory processes that induce fibrosis. In multiple studies, we screened various formulations and microencapsulating methods, and obtained promising results using bile acid-based microcapsules containing ß-cells, in terms of cell functions and insulin release. Thus, this study aimed to refine further the microencapsulating method using a simple alginate-poly-l-ornithine formulation and test the effect of adding a promising bile acid, ursodeoxycholic acid (UDCA), on cell functions. Using Büchi concentric nozzle, viable NIT-1 cells were microencapsulated using alginate-poly-l-ornithine, with or without UDCA at a ratio of 1:1.2 or 1:1.2:4. Screening for nozzle temperature and nozzle-gelation bath distance was carried out to form best microcapsules. Microcapsules were cultured for 48 h and examined for size and surface morphology, chemical profiling and ß-cell viability. Culture supernatants were examined for insulin and inflammatory cytokines. When using 30 °C nozzle-temperature and 5 cm nozzle-gelation bath distance, in the presence of the bile acid, cell mitochondrial activities and insulin production were optimised. Under deployed microencapsulating method with nozzle-temperature of 30 °C and nozzle-gelation bath distance of 5 cm, the incorporation of the bile acid into the microcapsules resulted in enhanced ß-cell survival, function and improved overall biocompatibility supporting potential applications in transplantation. 2016 Journal Article http://hdl.handle.net/20.500.11937/15066 10.1007/s12195-016-0441-z Springer New York LLC restricted |
| spellingShingle | Mooranian, Armin Negrulj, Rebecca Jamieson, E. Morahan, G. Al-Salami, Hani Biological Assessments of Encapsulated Pancreatic ß-Cells: Their Potential Transplantation in Diabetes |
| title | Biological Assessments of Encapsulated Pancreatic ß-Cells: Their Potential Transplantation in Diabetes |
| title_full | Biological Assessments of Encapsulated Pancreatic ß-Cells: Their Potential Transplantation in Diabetes |
| title_fullStr | Biological Assessments of Encapsulated Pancreatic ß-Cells: Their Potential Transplantation in Diabetes |
| title_full_unstemmed | Biological Assessments of Encapsulated Pancreatic ß-Cells: Their Potential Transplantation in Diabetes |
| title_short | Biological Assessments of Encapsulated Pancreatic ß-Cells: Their Potential Transplantation in Diabetes |
| title_sort | biological assessments of encapsulated pancreatic ß-cells: their potential transplantation in diabetes |
| url | http://hdl.handle.net/20.500.11937/15066 |