Genetic characterization of Listeria monocytogenes isolates from food processing facilities before and after postcook chiller heat treatment
Possible selection for and establishment of stress-resistant Listeria monocytogenes variants as a consequence of heating interventions is of concern to the food industry. Lineage analysis and multilocus variable number tandem repeat analysis (MLVA) was performed on 20 L. monocytogenes isolates, of w...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
2013
|
| Online Access: | http://hdl.handle.net/20.500.11937/14835 |
| _version_ | 1848748729174065152 |
|---|---|
| author | Eglezos, S. Dykes, Gary Huang, B. Turner, M. Seale, R. |
| author_facet | Eglezos, S. Dykes, Gary Huang, B. Turner, M. Seale, R. |
| author_sort | Eglezos, S. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Possible selection for and establishment of stress-resistant Listeria monocytogenes variants as a consequence of heating interventions is of concern to the food industry. Lineage analysis and multilocus variable number tandem repeat analysis (MLVA) was performed on 20 L. monocytogenes isolates, of which 15 were obtained before and 5 were obtained after heat treatment of a postcook meat chiller. The ctsR gene (a class III heat shock gene regulator) from 14 isolates was amplified and sequenced because previous work has indicated that spontaneous mutations can occur in this gene during heat treatment. Heat treatment of the meat chiller did not significantly change the relative abundance of the various L. monocytogenes lineages; lineage II strains (less-heat-resistant isolates) dominated both before and after heat treatment. MLVA typing confirmed that some isolates of L. monocytogenes occur both before and after heat treatment of the chiller. No isolate of L. monocytogenes indicated any likely functionally significant mutations in ctsR. This study indicates the absence of any obvious difference in the profiles of L. monocytogenes strains obtained before and after heat treatment of a meat chiller, based on the characteristics examined. Although this finding supports the effectiveness of heat treatment, the limited number of strains used and characteristics examined mean that further study on a larger scale is required before firm conclusions can be drawn. |
| first_indexed | 2025-11-14T07:09:40Z |
| format | Journal Article |
| id | curtin-20.500.11937-14835 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:09:40Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-148352017-09-13T14:07:13Z Genetic characterization of Listeria monocytogenes isolates from food processing facilities before and after postcook chiller heat treatment Eglezos, S. Dykes, Gary Huang, B. Turner, M. Seale, R. Possible selection for and establishment of stress-resistant Listeria monocytogenes variants as a consequence of heating interventions is of concern to the food industry. Lineage analysis and multilocus variable number tandem repeat analysis (MLVA) was performed on 20 L. monocytogenes isolates, of which 15 were obtained before and 5 were obtained after heat treatment of a postcook meat chiller. The ctsR gene (a class III heat shock gene regulator) from 14 isolates was amplified and sequenced because previous work has indicated that spontaneous mutations can occur in this gene during heat treatment. Heat treatment of the meat chiller did not significantly change the relative abundance of the various L. monocytogenes lineages; lineage II strains (less-heat-resistant isolates) dominated both before and after heat treatment. MLVA typing confirmed that some isolates of L. monocytogenes occur both before and after heat treatment of the chiller. No isolate of L. monocytogenes indicated any likely functionally significant mutations in ctsR. This study indicates the absence of any obvious difference in the profiles of L. monocytogenes strains obtained before and after heat treatment of a meat chiller, based on the characteristics examined. Although this finding supports the effectiveness of heat treatment, the limited number of strains used and characteristics examined mean that further study on a larger scale is required before firm conclusions can be drawn. 2013 Journal Article http://hdl.handle.net/20.500.11937/14835 10.4315/0362-028X.JFP-12-523 restricted |
| spellingShingle | Eglezos, S. Dykes, Gary Huang, B. Turner, M. Seale, R. Genetic characterization of Listeria monocytogenes isolates from food processing facilities before and after postcook chiller heat treatment |
| title | Genetic characterization of Listeria monocytogenes isolates from food processing facilities before and after postcook chiller heat treatment |
| title_full | Genetic characterization of Listeria monocytogenes isolates from food processing facilities before and after postcook chiller heat treatment |
| title_fullStr | Genetic characterization of Listeria monocytogenes isolates from food processing facilities before and after postcook chiller heat treatment |
| title_full_unstemmed | Genetic characterization of Listeria monocytogenes isolates from food processing facilities before and after postcook chiller heat treatment |
| title_short | Genetic characterization of Listeria monocytogenes isolates from food processing facilities before and after postcook chiller heat treatment |
| title_sort | genetic characterization of listeria monocytogenes isolates from food processing facilities before and after postcook chiller heat treatment |
| url | http://hdl.handle.net/20.500.11937/14835 |