Bacillus megaterium mediated mineralization of calcium carbonate as biogenic surface treatment of green building materials

Microbially induced calcium carbonate precipitation is a biomineralization process that has various applications in remediation and restoration of range of building materials. In the present study, calcifying bacteria, Bacillus megaterium SS3 isolated from calcareous soil was applied as biosealant t...

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Main Authors: Dhami, N., Reddy, M., Mukherjee, Abhijit
Format: Journal Article
Published: Springer Netherlands 2013
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/14348
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author Dhami, N.
Reddy, M.
Mukherjee, Abhijit
author_facet Dhami, N.
Reddy, M.
Mukherjee, Abhijit
author_sort Dhami, N.
building Curtin Institutional Repository
collection Online Access
description Microbially induced calcium carbonate precipitation is a biomineralization process that has various applications in remediation and restoration of range of building materials. In the present study, calcifying bacteria, Bacillus megaterium SS3 isolated from calcareous soil was applied as biosealant to enhance the durability of low energy, green building materials (soil-cement blocks). This bacterial isolate produced high amounts of urease, carbonic anhydrase, extra polymeric substances and biofilm. The calcium carbonate polymorphs produced by B. megaterium SS3 were analyzed by scanning electron microscopy, confocal laser scanning microscopy, X-ray diffraction and Fourier transmission infra red spectroscopy. These results suggested that calcite is the most predominant carbonate formed by this bacteria followed by vaterite. Application of B. megaterium SS3 as biogenic surface treatment led to 40 % decrease in water absorption, 31 % decrease in porosity and 18 % increase in compressive strength of low energy building materials. From the present investigation, it is clear that surface treatment of building materials by B. megaterium SS3 is very effective and eco friendly way of biodeposition of coherent carbonates that enhances the durability of building materials.
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institution Curtin University Malaysia
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publishDate 2013
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spelling curtin-20.500.11937-143482017-09-13T14:06:28Z Bacillus megaterium mediated mineralization of calcium carbonate as biogenic surface treatment of green building materials Dhami, N. Reddy, M. Mukherjee, Abhijit Extra polymeric substances Biofilm Urease Biomineralization Bacillus Green buildings Microbially induced calcium carbonate precipitation is a biomineralization process that has various applications in remediation and restoration of range of building materials. In the present study, calcifying bacteria, Bacillus megaterium SS3 isolated from calcareous soil was applied as biosealant to enhance the durability of low energy, green building materials (soil-cement blocks). This bacterial isolate produced high amounts of urease, carbonic anhydrase, extra polymeric substances and biofilm. The calcium carbonate polymorphs produced by B. megaterium SS3 were analyzed by scanning electron microscopy, confocal laser scanning microscopy, X-ray diffraction and Fourier transmission infra red spectroscopy. These results suggested that calcite is the most predominant carbonate formed by this bacteria followed by vaterite. Application of B. megaterium SS3 as biogenic surface treatment led to 40 % decrease in water absorption, 31 % decrease in porosity and 18 % increase in compressive strength of low energy building materials. From the present investigation, it is clear that surface treatment of building materials by B. megaterium SS3 is very effective and eco friendly way of biodeposition of coherent carbonates that enhances the durability of building materials. 2013 Journal Article http://hdl.handle.net/20.500.11937/14348 10.1007/s11274-013-1408-z Springer Netherlands restricted
spellingShingle Extra polymeric substances
Biofilm
Urease
Biomineralization
Bacillus
Green buildings
Dhami, N.
Reddy, M.
Mukherjee, Abhijit
Bacillus megaterium mediated mineralization of calcium carbonate as biogenic surface treatment of green building materials
title Bacillus megaterium mediated mineralization of calcium carbonate as biogenic surface treatment of green building materials
title_full Bacillus megaterium mediated mineralization of calcium carbonate as biogenic surface treatment of green building materials
title_fullStr Bacillus megaterium mediated mineralization of calcium carbonate as biogenic surface treatment of green building materials
title_full_unstemmed Bacillus megaterium mediated mineralization of calcium carbonate as biogenic surface treatment of green building materials
title_short Bacillus megaterium mediated mineralization of calcium carbonate as biogenic surface treatment of green building materials
title_sort bacillus megaterium mediated mineralization of calcium carbonate as biogenic surface treatment of green building materials
topic Extra polymeric substances
Biofilm
Urease
Biomineralization
Bacillus
Green buildings
url http://hdl.handle.net/20.500.11937/14348