The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved syteny with Medicago truncatula

We report the first gene-based linkage map of Lupinus angustifolius (narrow-leafed lupin) and its comparison to the partially sequenced genome of Medicago truncatula. The map comprises 382 loci in 20 major linkage groups, two triplets, three pairs and 11 unlinked loci and is 1,846 cM in length. The...

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Main Authors: Nelson, M., Phan, H., Hane, James, Williams, A., O'Lone, C., Fosu-Nyarko, J., Scobie, M., Cakir, M., Jones, M., Bellgard, M., Ksiazkiewicz, M., Wolko, B.W., Barker, S., Oliver, Richard, Cowling, W.
Format: Journal Article
Published: Springer 2006
Online Access:http://hdl.handle.net/20.500.11937/13797
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author Nelson, M.
Phan, H.
Hane, James
Williams, A.
O'Lone, C.
Fosu-Nyarko, J.
Scobie, M.
Cakir, M.
Jones, M.
Bellgard, M.
Ksiazkiewicz, M.
Wolko, B.W.
Barker, S.
Oliver, Richard
Cowling, W.
author_facet Nelson, M.
Phan, H.
Hane, James
Williams, A.
O'Lone, C.
Fosu-Nyarko, J.
Scobie, M.
Cakir, M.
Jones, M.
Bellgard, M.
Ksiazkiewicz, M.
Wolko, B.W.
Barker, S.
Oliver, Richard
Cowling, W.
author_sort Nelson, M.
building Curtin Institutional Repository
collection Online Access
description We report the first gene-based linkage map of Lupinus angustifolius (narrow-leafed lupin) and its comparison to the partially sequenced genome of Medicago truncatula. The map comprises 382 loci in 20 major linkage groups, two triplets, three pairs and 11 unlinked loci and is 1,846 cM in length. The map was generated from the segregation of 163 RFLP markers, 135 gene-based PCR markers, 75 AFLP and 4 AFLP-derived SCAR markers in a mapping population of 93 recombinant inbred lines, derived from a cross between domesticated and wild-type parents. This enabled the mapping of five major genes controlling key domestication traits in L. angustifolius. Using marker sequence data, the L. angustifolius genetic map was compared to the partially completed M. truncatula genome sequence. We found evidence of conserved synteny in some regions of the genome despite the wide evolutionary distance between these legume species. We also found new evidence of widespread duplication within the L. angustifolius genome.
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institution Curtin University Malaysia
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publishDate 2006
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spelling curtin-20.500.11937-137972020-06-29T06:48:53Z The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved syteny with Medicago truncatula Nelson, M. Phan, H. Hane, James Williams, A. O'Lone, C. Fosu-Nyarko, J. Scobie, M. Cakir, M. Jones, M. Bellgard, M. Ksiazkiewicz, M. Wolko, B.W. Barker, S. Oliver, Richard Cowling, W. We report the first gene-based linkage map of Lupinus angustifolius (narrow-leafed lupin) and its comparison to the partially sequenced genome of Medicago truncatula. The map comprises 382 loci in 20 major linkage groups, two triplets, three pairs and 11 unlinked loci and is 1,846 cM in length. The map was generated from the segregation of 163 RFLP markers, 135 gene-based PCR markers, 75 AFLP and 4 AFLP-derived SCAR markers in a mapping population of 93 recombinant inbred lines, derived from a cross between domesticated and wild-type parents. This enabled the mapping of five major genes controlling key domestication traits in L. angustifolius. Using marker sequence data, the L. angustifolius genetic map was compared to the partially completed M. truncatula genome sequence. We found evidence of conserved synteny in some regions of the genome despite the wide evolutionary distance between these legume species. We also found new evidence of widespread duplication within the L. angustifolius genome. 2006 Journal Article http://hdl.handle.net/20.500.11937/13797 10.1007/s00122-006-0288-0 Springer restricted
spellingShingle Nelson, M.
Phan, H.
Hane, James
Williams, A.
O'Lone, C.
Fosu-Nyarko, J.
Scobie, M.
Cakir, M.
Jones, M.
Bellgard, M.
Ksiazkiewicz, M.
Wolko, B.W.
Barker, S.
Oliver, Richard
Cowling, W.
The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved syteny with Medicago truncatula
title The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved syteny with Medicago truncatula
title_full The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved syteny with Medicago truncatula
title_fullStr The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved syteny with Medicago truncatula
title_full_unstemmed The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved syteny with Medicago truncatula
title_short The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved syteny with Medicago truncatula
title_sort first gene-based map of lupinus angustifolius l.-location of domestication genes and conserved syteny with medicago truncatula
url http://hdl.handle.net/20.500.11937/13797