Sensitivity to three Parastagonospora nodorum necrotrophic effectors in current Australian wheat cultivars and the presence of further fungal effectors
Parastagonospora nodorum is a major fungal pathogen of wheat in Australia causing septoria nodorum blotch (SNB). P. nodorum virulence is quantitative and depends to a large extent on multiple effector-host sensitivity gene interactions. The pathogen utilises a series of proteinaceous necrotrophic ef...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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CSIRO Publishing
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/13731 |
| _version_ | 1848748424449490944 |
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| author | Tan, Kar-Chun Waters, O. Rybak, K. Antoni, E. Furuki, E. Oliver, Richard |
| author_facet | Tan, Kar-Chun Waters, O. Rybak, K. Antoni, E. Furuki, E. Oliver, Richard |
| author_sort | Tan, Kar-Chun |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Parastagonospora nodorum is a major fungal pathogen of wheat in Australia causing septoria nodorum blotch (SNB). P. nodorum virulence is quantitative and depends to a large extent on multiple effector-host sensitivity gene interactions. The pathogen utilises a series of proteinaceous necrotrophic effectors to facilitate disease development on wheat cultivars that possess appropriate dominant sensitivity loci. Thus far, three necrotrophic effector genes have been cloned. Proteins derived from these genes were used to identify wheat cultivars that confer effector sensitivity. The goal of the study was to determine if effector sensitivity could be used to enhance breeding for SNB resistance. In this study, we have demonstrated that SnTox1 effector sensitivity is common in current commercial Western Australian wheat cultivars. Thirty-three of 46 cultivars showed evidence of sensitivity to SnTox1. Of these, 19 showed moderate or strong chlorotic/necrotic responses to SnTox1. Thirteen were completely insensitive to SnTox1. Disease susceptibility was most closely associated with SnTox3 sensitivity. In addition, we have identified biochemical evidence of a novel chlorosis-inducing protein or proteins in P. nodorum culture filtrates unmasked in strains that lack expression of ToxA, SnTox1 and SnTox3 activities. |
| first_indexed | 2025-11-14T07:04:49Z |
| format | Journal Article |
| id | curtin-20.500.11937-13731 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:04:49Z |
| publishDate | 2013 |
| publisher | CSIRO Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-137312017-09-13T16:06:24Z Sensitivity to three Parastagonospora nodorum necrotrophic effectors in current Australian wheat cultivars and the presence of further fungal effectors Tan, Kar-Chun Waters, O. Rybak, K. Antoni, E. Furuki, E. Oliver, Richard wheat SnTox3 necrotrophic effector (NE) SnTox1 ToxA septoria nodorum blotch Parastagonospora nodorum is a major fungal pathogen of wheat in Australia causing septoria nodorum blotch (SNB). P. nodorum virulence is quantitative and depends to a large extent on multiple effector-host sensitivity gene interactions. The pathogen utilises a series of proteinaceous necrotrophic effectors to facilitate disease development on wheat cultivars that possess appropriate dominant sensitivity loci. Thus far, three necrotrophic effector genes have been cloned. Proteins derived from these genes were used to identify wheat cultivars that confer effector sensitivity. The goal of the study was to determine if effector sensitivity could be used to enhance breeding for SNB resistance. In this study, we have demonstrated that SnTox1 effector sensitivity is common in current commercial Western Australian wheat cultivars. Thirty-three of 46 cultivars showed evidence of sensitivity to SnTox1. Of these, 19 showed moderate or strong chlorotic/necrotic responses to SnTox1. Thirteen were completely insensitive to SnTox1. Disease susceptibility was most closely associated with SnTox3 sensitivity. In addition, we have identified biochemical evidence of a novel chlorosis-inducing protein or proteins in P. nodorum culture filtrates unmasked in strains that lack expression of ToxA, SnTox1 and SnTox3 activities. 2013 Journal Article http://hdl.handle.net/20.500.11937/13731 10.1071/CP13443 CSIRO Publishing fulltext |
| spellingShingle | wheat SnTox3 necrotrophic effector (NE) SnTox1 ToxA septoria nodorum blotch Tan, Kar-Chun Waters, O. Rybak, K. Antoni, E. Furuki, E. Oliver, Richard Sensitivity to three Parastagonospora nodorum necrotrophic effectors in current Australian wheat cultivars and the presence of further fungal effectors |
| title | Sensitivity to three Parastagonospora nodorum necrotrophic effectors in current Australian wheat cultivars and the presence of further fungal effectors |
| title_full | Sensitivity to three Parastagonospora nodorum necrotrophic effectors in current Australian wheat cultivars and the presence of further fungal effectors |
| title_fullStr | Sensitivity to three Parastagonospora nodorum necrotrophic effectors in current Australian wheat cultivars and the presence of further fungal effectors |
| title_full_unstemmed | Sensitivity to three Parastagonospora nodorum necrotrophic effectors in current Australian wheat cultivars and the presence of further fungal effectors |
| title_short | Sensitivity to three Parastagonospora nodorum necrotrophic effectors in current Australian wheat cultivars and the presence of further fungal effectors |
| title_sort | sensitivity to three parastagonospora nodorum necrotrophic effectors in current australian wheat cultivars and the presence of further fungal effectors |
| topic | wheat SnTox3 necrotrophic effector (NE) SnTox1 ToxA septoria nodorum blotch |
| url | http://hdl.handle.net/20.500.11937/13731 |