Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi
A chimaeric β-glucuronidase (GUS) gene has been created by ligating the Aspergillus nidulans glyceraldehyde 3-phosphate dehydrogenase promoter to the coding sequence of the E. coli uidA gene. Cotransformation of this vector into A. nidulans, A. niger and the tomato pathogen Fulvia fulva (syn. Clados...
| Main Authors: | , , , |
|---|---|
| Format: | Journal Article |
| Published: |
1989
|
| Online Access: | http://hdl.handle.net/20.500.11937/13483 |
| _version_ | 1848748359628619776 |
|---|---|
| author | Roberts, I. Oliver, Richard Punt, P. Van Den Hondel, C. |
| author_facet | Roberts, I. Oliver, Richard Punt, P. Van Den Hondel, C. |
| author_sort | Roberts, I. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | A chimaeric β-glucuronidase (GUS) gene has been created by ligating the Aspergillus nidulans glyceraldehyde 3-phosphate dehydrogenase promoter to the coding sequence of the E. coli uidA gene. Cotransformation of this vector into A. nidulans, A. niger and the tomato pathogen Fulvia fulva (syn. Cladosporium fulvum (Cooke)) resulted in the expression of β-glucuronidase. GUS activity was detected by growth on agar media containing X-gluc and by enzyme assays of mycelial extracts. Expression of the gene in F. fulva transformants was also easily detectable during growth in plants and did not affect pathogenicity. These results form the basis for a versatile and sensitive reporter gene system for industrial and phytopathogenic filamentous fungi. |
| first_indexed | 2025-11-14T07:03:48Z |
| format | Journal Article |
| id | curtin-20.500.11937-13483 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:03:48Z |
| publishDate | 1989 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-134832017-09-13T16:02:00Z Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi Roberts, I. Oliver, Richard Punt, P. Van Den Hondel, C. A chimaeric β-glucuronidase (GUS) gene has been created by ligating the Aspergillus nidulans glyceraldehyde 3-phosphate dehydrogenase promoter to the coding sequence of the E. coli uidA gene. Cotransformation of this vector into A. nidulans, A. niger and the tomato pathogen Fulvia fulva (syn. Cladosporium fulvum (Cooke)) resulted in the expression of β-glucuronidase. GUS activity was detected by growth on agar media containing X-gluc and by enzyme assays of mycelial extracts. Expression of the gene in F. fulva transformants was also easily detectable during growth in plants and did not affect pathogenicity. These results form the basis for a versatile and sensitive reporter gene system for industrial and phytopathogenic filamentous fungi. 1989 Journal Article http://hdl.handle.net/20.500.11937/13483 10.1007/BF00435503 restricted |
| spellingShingle | Roberts, I. Oliver, Richard Punt, P. Van Den Hondel, C. Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi |
| title | Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi |
| title_full | Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi |
| title_fullStr | Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi |
| title_full_unstemmed | Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi |
| title_short | Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi |
| title_sort | expression of the escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi |
| url | http://hdl.handle.net/20.500.11937/13483 |