Identification and characterisation of ten microsatellite loci in the Noisy Scrub-bird Atrichornis clamosus using next-generation sequencing technology
The Noisy Scrub-bird is an endangered species of songbird endemic to the south coast of Western Australia that has undergone a major and prolonged population bottleneck. Using shotgun 454 next-generation DNA sequencing we have identified and characterised ten polymorphic microsatellite loci in this...
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| Format: | Journal Article |
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Springer
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/13377 |
| _version_ | 1848748331566628864 |
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| author | Cowen, Saul Allcock, R. Comer, S. Wetherall, John Groth, D. |
| author_facet | Cowen, Saul Allcock, R. Comer, S. Wetherall, John Groth, D. |
| author_sort | Cowen, Saul |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The Noisy Scrub-bird is an endangered species of songbird endemic to the south coast of Western Australia that has undergone a major and prolonged population bottleneck. Using shotgun 454 next-generation DNA sequencing we have identified and characterised ten polymorphic microsatellite loci in this species. Observed allelic diversity was relatively low (2–5 alleles per locus) and significant deviations from Hardy–weinberg Equilibrium observed, although the presence of null alleles was onlypostulated for two loci. The microsatellite loci characterised in this study will be useful in a future population genetics studies in this endangered species. |
| first_indexed | 2025-11-14T07:03:21Z |
| format | Journal Article |
| id | curtin-20.500.11937-13377 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:03:21Z |
| publishDate | 2013 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-133772017-09-13T16:06:24Z Identification and characterisation of ten microsatellite loci in the Noisy Scrub-bird Atrichornis clamosus using next-generation sequencing technology Cowen, Saul Allcock, R. Comer, S. Wetherall, John Groth, D. Microsatellites Short-tandem repeats Next-generation sequencing The Noisy Scrub-bird is an endangered species of songbird endemic to the south coast of Western Australia that has undergone a major and prolonged population bottleneck. Using shotgun 454 next-generation DNA sequencing we have identified and characterised ten polymorphic microsatellite loci in this species. Observed allelic diversity was relatively low (2–5 alleles per locus) and significant deviations from Hardy–weinberg Equilibrium observed, although the presence of null alleles was onlypostulated for two loci. The microsatellite loci characterised in this study will be useful in a future population genetics studies in this endangered species. 2013 Journal Article http://hdl.handle.net/20.500.11937/13377 10.1007/s12686-013-9865-0 Springer fulltext |
| spellingShingle | Microsatellites Short-tandem repeats Next-generation sequencing Cowen, Saul Allcock, R. Comer, S. Wetherall, John Groth, D. Identification and characterisation of ten microsatellite loci in the Noisy Scrub-bird Atrichornis clamosus using next-generation sequencing technology |
| title | Identification and characterisation of ten microsatellite loci in the Noisy Scrub-bird Atrichornis clamosus using next-generation sequencing technology |
| title_full | Identification and characterisation of ten microsatellite loci in the Noisy Scrub-bird Atrichornis clamosus using next-generation sequencing technology |
| title_fullStr | Identification and characterisation of ten microsatellite loci in the Noisy Scrub-bird Atrichornis clamosus using next-generation sequencing technology |
| title_full_unstemmed | Identification and characterisation of ten microsatellite loci in the Noisy Scrub-bird Atrichornis clamosus using next-generation sequencing technology |
| title_short | Identification and characterisation of ten microsatellite loci in the Noisy Scrub-bird Atrichornis clamosus using next-generation sequencing technology |
| title_sort | identification and characterisation of ten microsatellite loci in the noisy scrub-bird atrichornis clamosus using next-generation sequencing technology |
| topic | Microsatellites Short-tandem repeats Next-generation sequencing |
| url | http://hdl.handle.net/20.500.11937/13377 |