Biocompatibility of semiconducting AlGaN/GaN material with living cells
With the aim of developing a highly sensitive, mass producible biosensor, we have investigated the growth of human embryonic kidney (HEK) 293 cells on the surface of semiconductor grade AlGaN/GaN heterostructures. Our results demonstrate that, even without specialised surface treatment, a substantia...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
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Elsevier SA
2012
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| Online Access: | http://hdl.handle.net/20.500.11937/12988 |
| _version_ | 1848748228474830848 |
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| author | Podolska, Anna Tham, S. Hart, Robert Seeber, R. Kocan, M. Kocan, M. Mishra, U. Pfleger, K. Parish, G. Nener, B. |
| author_facet | Podolska, Anna Tham, S. Hart, Robert Seeber, R. Kocan, M. Kocan, M. Mishra, U. Pfleger, K. Parish, G. Nener, B. |
| author_sort | Podolska, Anna |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | With the aim of developing a highly sensitive, mass producible biosensor, we have investigated the growth of human embryonic kidney (HEK) 293 cells on the surface of semiconductor grade AlGaN/GaN heterostructures. Our results demonstrate that, even without specialised surface treatment, a substantial amount of attachment and proliferation of cells is observed. Growth and mortality rates on the AlGaN surface were comparable to standard control culture plates. Quantitative studies of mortality measured by flow cytometry correlate well with qualitative monitoring of biocompatibility. The percentage of dead cells increases marginally with increasing Al concentration. Cell attachment was investigated qualitatively using focused ion beam/scanning electron microscopy (FIB/SEM) and transmission electron microscopy (TEM). Imaging showed strong attachment at the cell/semiconductor interface at the nanometre level. These measurements are the first study of live cell/semiconductor interactions using complementary methods for proliferation, mortality, and attachment, and confirm that the combination of live cells as the biosensing element and AlGaN/GaN heterostructures as the transducer has significant potential for biosensor applications. |
| first_indexed | 2025-11-14T07:01:42Z |
| format | Journal Article |
| id | curtin-20.500.11937-12988 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:01:42Z |
| publishDate | 2012 |
| publisher | Elsevier SA |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-129882017-09-13T15:01:02Z Biocompatibility of semiconducting AlGaN/GaN material with living cells Podolska, Anna Tham, S. Hart, Robert Seeber, R. Kocan, M. Kocan, M. Mishra, U. Pfleger, K. Parish, G. Nener, B. Biocompatibility Cell/semiconductor interface AlGaN/GaN Cell-based biosensor HEK293 With the aim of developing a highly sensitive, mass producible biosensor, we have investigated the growth of human embryonic kidney (HEK) 293 cells on the surface of semiconductor grade AlGaN/GaN heterostructures. Our results demonstrate that, even without specialised surface treatment, a substantial amount of attachment and proliferation of cells is observed. Growth and mortality rates on the AlGaN surface were comparable to standard control culture plates. Quantitative studies of mortality measured by flow cytometry correlate well with qualitative monitoring of biocompatibility. The percentage of dead cells increases marginally with increasing Al concentration. Cell attachment was investigated qualitatively using focused ion beam/scanning electron microscopy (FIB/SEM) and transmission electron microscopy (TEM). Imaging showed strong attachment at the cell/semiconductor interface at the nanometre level. These measurements are the first study of live cell/semiconductor interactions using complementary methods for proliferation, mortality, and attachment, and confirm that the combination of live cells as the biosensing element and AlGaN/GaN heterostructures as the transducer has significant potential for biosensor applications. 2012 Journal Article http://hdl.handle.net/20.500.11937/12988 10.1016/j.snb.2012.04.015 Elsevier SA restricted |
| spellingShingle | Biocompatibility Cell/semiconductor interface AlGaN/GaN Cell-based biosensor HEK293 Podolska, Anna Tham, S. Hart, Robert Seeber, R. Kocan, M. Kocan, M. Mishra, U. Pfleger, K. Parish, G. Nener, B. Biocompatibility of semiconducting AlGaN/GaN material with living cells |
| title | Biocompatibility of semiconducting AlGaN/GaN material with living cells |
| title_full | Biocompatibility of semiconducting AlGaN/GaN material with living cells |
| title_fullStr | Biocompatibility of semiconducting AlGaN/GaN material with living cells |
| title_full_unstemmed | Biocompatibility of semiconducting AlGaN/GaN material with living cells |
| title_short | Biocompatibility of semiconducting AlGaN/GaN material with living cells |
| title_sort | biocompatibility of semiconducting algan/gan material with living cells |
| topic | Biocompatibility Cell/semiconductor interface AlGaN/GaN Cell-based biosensor HEK293 |
| url | http://hdl.handle.net/20.500.11937/12988 |