Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections
The Chelonid fibropapilloma-associated herpesvirus (CFPHV) is hypothesized to be the causative agent of fibropapillomatosis, a neoplastic disease in sea turtles, given its consistent detection by PCR in fibropapilloma tumours. CFPHV has also been detected recently by PCR in tissue samples from clini...
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| Format: | Journal Article |
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Elsevier BV
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/12708 |
| _version_ | 1848748152434196480 |
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| author | Alfaro-Núñez, A. Gilbert, Thomas |
| author_facet | Alfaro-Núñez, A. Gilbert, Thomas |
| author_sort | Alfaro-Núñez, A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The Chelonid fibropapilloma-associated herpesvirus (CFPHV) is hypothesized to be the causative agent of fibropapillomatosis, a neoplastic disease in sea turtles, given its consistent detection by PCR in fibropapilloma tumours. CFPHV has also been detected recently by PCR in tissue samples from clinically healthy (non exhibiting fibropapilloma tumours) turtles, thus representing presumably latent infections of the pathogen. Given that template copy numbers of viruses in latent infections can be very low, extremely sensitive PCR assays are needed to optimize detection efficiency. In this study, efficiency of several PCR assays designed for CFPHV detection is explored and compared to a method published previously. The results show that adoption of a triplet set of singleplex PCR assays outperforms other methods, with an approximately 3-fold increase in detection success in comparison to the standard assay. Thus, a new assay for the detection of CFPHV DNA markers is presented, and adoption of its methodology is recommended in future CFPHV screens among sea turtles. |
| first_indexed | 2025-11-14T07:00:30Z |
| format | Journal Article |
| id | curtin-20.500.11937-12708 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:00:30Z |
| publishDate | 2014 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-127082017-09-13T15:01:03Z Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections Alfaro-Núñez, A. Gilbert, Thomas Latency Chelonid fibropapilloma-associated herpesvirus Sensitivity Sea turtles Fibropapillomatosis PCR The Chelonid fibropapilloma-associated herpesvirus (CFPHV) is hypothesized to be the causative agent of fibropapillomatosis, a neoplastic disease in sea turtles, given its consistent detection by PCR in fibropapilloma tumours. CFPHV has also been detected recently by PCR in tissue samples from clinically healthy (non exhibiting fibropapilloma tumours) turtles, thus representing presumably latent infections of the pathogen. Given that template copy numbers of viruses in latent infections can be very low, extremely sensitive PCR assays are needed to optimize detection efficiency. In this study, efficiency of several PCR assays designed for CFPHV detection is explored and compared to a method published previously. The results show that adoption of a triplet set of singleplex PCR assays outperforms other methods, with an approximately 3-fold increase in detection success in comparison to the standard assay. Thus, a new assay for the detection of CFPHV DNA markers is presented, and adoption of its methodology is recommended in future CFPHV screens among sea turtles. 2014 Journal Article http://hdl.handle.net/20.500.11937/12708 10.1016/j.jviromet.2014.05.019 Elsevier BV restricted |
| spellingShingle | Latency Chelonid fibropapilloma-associated herpesvirus Sensitivity Sea turtles Fibropapillomatosis PCR Alfaro-Núñez, A. Gilbert, Thomas Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections |
| title | Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections |
| title_full | Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections |
| title_fullStr | Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections |
| title_full_unstemmed | Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections |
| title_short | Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections |
| title_sort | validation of a sensitive pcr assay for the detection of chelonid fibropapilloma-associated herpesvirus in latent turtle infections |
| topic | Latency Chelonid fibropapilloma-associated herpesvirus Sensitivity Sea turtles Fibropapillomatosis PCR |
| url | http://hdl.handle.net/20.500.11937/12708 |