Target DNA recognition using electrochemical impedance spectroscopy
Electrochemical impedance spectroscopy was used to differentiate between different target DNA sequences without the use of a redox label or application of large bias voltages. This method relies on the formation of a mixed self-assembled monolayer (SAMs) composed of single-stranded DNA and a diluent...
| Main Authors: | , , , |
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| Format: | Conference Paper |
| Published: |
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/12630 |
| Summary: | Electrochemical impedance spectroscopy was used to differentiate between different target DNA sequences without the use of a redox label or application of large bias voltages. This method relies on the formation of a mixed self-assembled monolayer (SAMs) composed of single-stranded DNA and a diluent, mercaptoethanol. Recognition of the DNA analytes was achieved through the significant increase in conductance for the double stranded DNA, as compared to single-stranded DNA. Non-complementary and single base-pair mismatches were also able to be differentiated. © 2010 IEEE. |
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