Amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposites for enhanced gene delivery

In this work a novel strategy has been developed to prepare well-dispersed amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposite (NH2-SiO2@LDH) via electrostatic interactions and condensation of (3-aminopropyl)triethoxysilane (APTES). This nanocomposite system is well dispe...

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Main Authors: Li, L., Gu, W., Liu, Jian, Yan, S., Xu, Z.
Format: Journal Article
Published: Tsinghua University Press 2015
Online Access:http://hdl.handle.net/20.500.11937/12574
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author Li, L.
Gu, W.
Liu, Jian
Yan, S.
Xu, Z.
author_facet Li, L.
Gu, W.
Liu, Jian
Yan, S.
Xu, Z.
author_sort Li, L.
building Curtin Institutional Repository
collection Online Access
description In this work a novel strategy has been developed to prepare well-dispersed amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposite (NH2-SiO2@LDH) via electrostatic interactions and condensation of (3-aminopropyl)triethoxysilane (APTES). This nanocomposite system is well dispersed in culture media and phosphate buffered saline, and exhibits low cytotoxicity and good biocompatibility. The fluorescence microscopy images and flow cytometry data indicate that such an NH2-SiO2@LDH nanocomposite is able to efficiently deliver small interfering RNA (siRNA) into the U2OS cell line to inhibit cell proliferation. Thus, NH2-SiO2@LDH nanocomposite has a great potential as a nanocarrier for efficient gene delivery.
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format Journal Article
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institution Curtin University Malaysia
institution_category Local University
last_indexed 2025-11-14T06:59:51Z
publishDate 2015
publisher Tsinghua University Press
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spelling curtin-20.500.11937-125742017-09-13T14:56:52Z Amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposites for enhanced gene delivery Li, L. Gu, W. Liu, Jian Yan, S. Xu, Z. In this work a novel strategy has been developed to prepare well-dispersed amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposite (NH2-SiO2@LDH) via electrostatic interactions and condensation of (3-aminopropyl)triethoxysilane (APTES). This nanocomposite system is well dispersed in culture media and phosphate buffered saline, and exhibits low cytotoxicity and good biocompatibility. The fluorescence microscopy images and flow cytometry data indicate that such an NH2-SiO2@LDH nanocomposite is able to efficiently deliver small interfering RNA (siRNA) into the U2OS cell line to inhibit cell proliferation. Thus, NH2-SiO2@LDH nanocomposite has a great potential as a nanocarrier for efficient gene delivery. 2015 Journal Article http://hdl.handle.net/20.500.11937/12574 10.1007/s12274-014-0552-6 Tsinghua University Press restricted
spellingShingle Li, L.
Gu, W.
Liu, Jian
Yan, S.
Xu, Z.
Amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposites for enhanced gene delivery
title Amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposites for enhanced gene delivery
title_full Amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposites for enhanced gene delivery
title_fullStr Amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposites for enhanced gene delivery
title_full_unstemmed Amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposites for enhanced gene delivery
title_short Amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposites for enhanced gene delivery
title_sort amine-functionalized sio2 nanodot-coated layered double hydroxide nanocomposites for enhanced gene delivery
url http://hdl.handle.net/20.500.11937/12574