A unique wheat disease resistance-like gene governs effector-triggered susceptibility to necrotrophic pathogens
Plant disease resistance is often conferred by genes with nucleotide binding site (NBS) and leucine-rich repeat (LRR) or serine/threonine protein kinase (S/TPK) domains. Much less is known about mechanisms of susceptibility, particularly to necrotrophic fungal pathogens. The pathogens that cause the...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
National Academy of Sciences
2010
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/12471 |
| _version_ | 1848748085570699264 |
|---|---|
| author | Faris, J. Zhang, Z. Lu, H. Lu, S. Reddy, L. Cloutier, S. Fellers, J. Meinhardt, S. Rasmussen, J. Xu, S. Oliver, Richard Simons, K. Friesen, T. |
| author_facet | Faris, J. Zhang, Z. Lu, H. Lu, S. Reddy, L. Cloutier, S. Fellers, J. Meinhardt, S. Rasmussen, J. Xu, S. Oliver, Richard Simons, K. Friesen, T. |
| author_sort | Faris, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Plant disease resistance is often conferred by genes with nucleotide binding site (NBS) and leucine-rich repeat (LRR) or serine/threonine protein kinase (S/TPK) domains. Much less is known about mechanisms of susceptibility, particularly to necrotrophic fungal pathogens. The pathogens that cause the diseases tan spot and Stagonospora nodorum blotch on wheat produce effectors (host-selective toxins) that induce susceptibility in wheat lines harboring corresponding toxin sensitivity genes. The effector ToxA is produced by both pathogens, and sensitivity to ToxA is governed by the Tsn1 gene on wheat chromosome arm 5BL. Here, we report the cloning of Tsn1, which was found to have disease resistance gene-like features, including S/TPK and NBS-LRR domains. Mutagenesis revealed that all three domains are required for ToxA sensitivity, and hence disease susceptibility. Tsn1 is unique to ToxA-sensitive genotypes, and insensitive genotypes are null. Sequencing and phylogenetic analysis indicated that Tsn1 arose in the B-genome diploid progenitor of polyploid wheat through a gene-fusion event that gave rise to its unique structure. Although Tsn1 is necessary to mediate ToxA recognition, yeast two-hybrid experiments suggested that the Tsn1 protein does not interact directly with ToxA. Tsn1 transcription is tightly regulated by the circadian clock and light, providing further evidence that Tsn1-ToxA interactions are associated with photosynthesis pathways. This work suggests that these necrotrophic pathogens may thrive by subverting the resistance mechanisms acquired by plants to combat other pathogens. |
| first_indexed | 2025-11-14T06:59:26Z |
| format | Journal Article |
| id | curtin-20.500.11937-12471 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:59:26Z |
| publishDate | 2010 |
| publisher | National Academy of Sciences |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-124712017-09-13T14:56:52Z A unique wheat disease resistance-like gene governs effector-triggered susceptibility to necrotrophic pathogens Faris, J. Zhang, Z. Lu, H. Lu, S. Reddy, L. Cloutier, S. Fellers, J. Meinhardt, S. Rasmussen, J. Xu, S. Oliver, Richard Simons, K. Friesen, T. map-based cloning disease resistance host–pathogen interaction Triticum necrotrophic fungus Plant disease resistance is often conferred by genes with nucleotide binding site (NBS) and leucine-rich repeat (LRR) or serine/threonine protein kinase (S/TPK) domains. Much less is known about mechanisms of susceptibility, particularly to necrotrophic fungal pathogens. The pathogens that cause the diseases tan spot and Stagonospora nodorum blotch on wheat produce effectors (host-selective toxins) that induce susceptibility in wheat lines harboring corresponding toxin sensitivity genes. The effector ToxA is produced by both pathogens, and sensitivity to ToxA is governed by the Tsn1 gene on wheat chromosome arm 5BL. Here, we report the cloning of Tsn1, which was found to have disease resistance gene-like features, including S/TPK and NBS-LRR domains. Mutagenesis revealed that all three domains are required for ToxA sensitivity, and hence disease susceptibility. Tsn1 is unique to ToxA-sensitive genotypes, and insensitive genotypes are null. Sequencing and phylogenetic analysis indicated that Tsn1 arose in the B-genome diploid progenitor of polyploid wheat through a gene-fusion event that gave rise to its unique structure. Although Tsn1 is necessary to mediate ToxA recognition, yeast two-hybrid experiments suggested that the Tsn1 protein does not interact directly with ToxA. Tsn1 transcription is tightly regulated by the circadian clock and light, providing further evidence that Tsn1-ToxA interactions are associated with photosynthesis pathways. This work suggests that these necrotrophic pathogens may thrive by subverting the resistance mechanisms acquired by plants to combat other pathogens. 2010 Journal Article http://hdl.handle.net/20.500.11937/12471 10.1073/pnas.1004090107 National Academy of Sciences fulltext |
| spellingShingle | map-based cloning disease resistance host–pathogen interaction Triticum necrotrophic fungus Faris, J. Zhang, Z. Lu, H. Lu, S. Reddy, L. Cloutier, S. Fellers, J. Meinhardt, S. Rasmussen, J. Xu, S. Oliver, Richard Simons, K. Friesen, T. A unique wheat disease resistance-like gene governs effector-triggered susceptibility to necrotrophic pathogens |
| title | A unique wheat disease resistance-like gene governs effector-triggered susceptibility to necrotrophic pathogens |
| title_full | A unique wheat disease resistance-like gene governs effector-triggered susceptibility to necrotrophic pathogens |
| title_fullStr | A unique wheat disease resistance-like gene governs effector-triggered susceptibility to necrotrophic pathogens |
| title_full_unstemmed | A unique wheat disease resistance-like gene governs effector-triggered susceptibility to necrotrophic pathogens |
| title_short | A unique wheat disease resistance-like gene governs effector-triggered susceptibility to necrotrophic pathogens |
| title_sort | unique wheat disease resistance-like gene governs effector-triggered susceptibility to necrotrophic pathogens |
| topic | map-based cloning disease resistance host–pathogen interaction Triticum necrotrophic fungus |
| url | http://hdl.handle.net/20.500.11937/12471 |