Determination of amino acids and amines in mammalian decomposition fluid by direct injection liquid chromatography-electrospray ionisation-tandem mass spectrometry
A sensitive and selective analytical method utilising liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS/MS) operated in multiple reaction monitoring mode was developed for the semi-quantitative determination of 19 biogenic amines and amino acids in mammalian (porcine)...
| Main Authors: | , , |
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| Format: | Journal Article |
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RSC Publishing
2012
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| Online Access: | http://hdl.handle.net/20.500.11937/12435 |
| _version_ | 1848748075457183744 |
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| author | Swann, Lisa Busetti, Francesco Lewis, Simon |
| author_facet | Swann, Lisa Busetti, Francesco Lewis, Simon |
| author_sort | Swann, Lisa |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | A sensitive and selective analytical method utilising liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS/MS) operated in multiple reaction monitoring mode was developed for the semi-quantitative determination of 19 biogenic amines and amino acids in mammalian (porcine) decomposition fluid. The effect of the matrix on analyte response was initially investigated by diluting crude samples and by injecting various volumes in the LC-MS system. It was found that the matrix had little effect on analyte signal intensities when small volumes (0.1 to 1 µL) of crude samples or 1 : 10 diluted samples were injected into the LC-ESI-MS/MS system. The standard addition method was also investigated for quantitative assessment but proved unsuccessful, possibly due to poor ionisation of the electrospray interface at high analyte concentrations. Therefore a method using external calibration was used for semi-quantitative determination of the analytes in decomposition fluid. The LC-ESI-MS/MS method enabled identification of all target compounds as being present. In addition a 14-day cyclic trend in the concentrations of two amino acids, phenylalanine and tryptophan, was tentatively established. General increasing trends with respect to time and temperature were also observed for putrescine and indole. |
| first_indexed | 2025-11-14T06:59:17Z |
| format | Journal Article |
| id | curtin-20.500.11937-12435 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:59:17Z |
| publishDate | 2012 |
| publisher | RSC Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-124352017-09-13T16:07:07Z Determination of amino acids and amines in mammalian decomposition fluid by direct injection liquid chromatography-electrospray ionisation-tandem mass spectrometry Swann, Lisa Busetti, Francesco Lewis, Simon A sensitive and selective analytical method utilising liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS/MS) operated in multiple reaction monitoring mode was developed for the semi-quantitative determination of 19 biogenic amines and amino acids in mammalian (porcine) decomposition fluid. The effect of the matrix on analyte response was initially investigated by diluting crude samples and by injecting various volumes in the LC-MS system. It was found that the matrix had little effect on analyte signal intensities when small volumes (0.1 to 1 µL) of crude samples or 1 : 10 diluted samples were injected into the LC-ESI-MS/MS system. The standard addition method was also investigated for quantitative assessment but proved unsuccessful, possibly due to poor ionisation of the electrospray interface at high analyte concentrations. Therefore a method using external calibration was used for semi-quantitative determination of the analytes in decomposition fluid. The LC-ESI-MS/MS method enabled identification of all target compounds as being present. In addition a 14-day cyclic trend in the concentrations of two amino acids, phenylalanine and tryptophan, was tentatively established. General increasing trends with respect to time and temperature were also observed for putrescine and indole. 2012 Journal Article http://hdl.handle.net/20.500.11937/12435 10.1039/c1ay05447c RSC Publishing restricted |
| spellingShingle | Swann, Lisa Busetti, Francesco Lewis, Simon Determination of amino acids and amines in mammalian decomposition fluid by direct injection liquid chromatography-electrospray ionisation-tandem mass spectrometry |
| title | Determination of amino acids and amines in mammalian decomposition fluid by direct injection liquid chromatography-electrospray ionisation-tandem mass spectrometry |
| title_full | Determination of amino acids and amines in mammalian decomposition fluid by direct injection liquid chromatography-electrospray ionisation-tandem mass spectrometry |
| title_fullStr | Determination of amino acids and amines in mammalian decomposition fluid by direct injection liquid chromatography-electrospray ionisation-tandem mass spectrometry |
| title_full_unstemmed | Determination of amino acids and amines in mammalian decomposition fluid by direct injection liquid chromatography-electrospray ionisation-tandem mass spectrometry |
| title_short | Determination of amino acids and amines in mammalian decomposition fluid by direct injection liquid chromatography-electrospray ionisation-tandem mass spectrometry |
| title_sort | determination of amino acids and amines in mammalian decomposition fluid by direct injection liquid chromatography-electrospray ionisation-tandem mass spectrometry |
| url | http://hdl.handle.net/20.500.11937/12435 |