Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva
A procedure for producing high yields and high regeneration frequencies of protoplasts from Fulvia fulva is described. This procedure was devised by systematic trials of various parameters, such as culture age and osmotic support, which are known to affect protoplast yield and regeneration. Mycelium...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Published: |
1988
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| Online Access: | http://hdl.handle.net/20.500.11937/12393 |
| _version_ | 1848748063946964992 |
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| author | Harling, R. Kenyon, L. Lewis, B. Oliver, Richard Turner, J. Coddington, A. |
| author_facet | Harling, R. Kenyon, L. Lewis, B. Oliver, Richard Turner, J. Coddington, A. |
| author_sort | Harling, R. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | A procedure for producing high yields and high regeneration frequencies of protoplasts from Fulvia fulva is described. This procedure was devised by systematic trials of various parameters, such as culture age and osmotic support, which are known to affect protoplast yield and regeneration. Mycelium from liquid cultures of 24–48 h, incubated with Novozym 234 in buffered 1.0 M MgSO4, gave the best conditions for protoplast release. Regeneration frequencies up to 50% were obtained with a complete medium containing 0.8 M sucrose for osmotic support. |
| first_indexed | 2025-11-14T06:59:06Z |
| format | Journal Article |
| id | curtin-20.500.11937-12393 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:59:06Z |
| publishDate | 1988 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-123932017-09-13T16:06:11Z Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva Harling, R. Kenyon, L. Lewis, B. Oliver, Richard Turner, J. Coddington, A. A procedure for producing high yields and high regeneration frequencies of protoplasts from Fulvia fulva is described. This procedure was devised by systematic trials of various parameters, such as culture age and osmotic support, which are known to affect protoplast yield and regeneration. Mycelium from liquid cultures of 24–48 h, incubated with Novozym 234 in buffered 1.0 M MgSO4, gave the best conditions for protoplast release. Regeneration frequencies up to 50% were obtained with a complete medium containing 0.8 M sucrose for osmotic support. 1988 Journal Article http://hdl.handle.net/20.500.11937/12393 10.1111/j.1439-0434.1988.tb01001.x restricted |
| spellingShingle | Harling, R. Kenyon, L. Lewis, B. Oliver, Richard Turner, J. Coddington, A. Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva |
| title | Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva |
| title_full | Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva |
| title_fullStr | Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva |
| title_full_unstemmed | Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva |
| title_short | Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva |
| title_sort | conditions for efficient isolation and regeneration of protoplasts from fulvia fulva |
| url | http://hdl.handle.net/20.500.11937/12393 |