One Hundred Twenty Years of Koala Retrovirus Evolution Determined from Museum Skins

Although endogenous retroviruses are common across vertebrate genomes, the koala retrovirus (KoRV) is the only retrovirus known to be currently invading the germ line of its host. KoRV is believed to have first infected koalas in northern Australia less than two centuries ago. We examined KoRV in 28...

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Main Authors: Avila-Arcos, M., Ho, S., Ishida, Y., Nikolaidis, N., Tsangaras, K., Honig, K., Medina, R., Rasmussen, M., Fordyce, S., Calvignac-Spencer, S., Willerslev, E., Gilbert, Thomas, Helgen, K., Roca, A., Greenwood, A.
Format: Journal Article
Published: Oxford Univ Press 2013
Subjects:
Online Access:http://mbe.oxfordjournals.org/content/30/2/299.full.pdf+html?sid=fdfdecf9-8b7d-487f-94ed-82613915e5c5
http://hdl.handle.net/20.500.11937/12304
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author Avila-Arcos, M.
Ho, S.
Ishida, Y.
Nikolaidis, N.
Tsangaras, K.
Honig, K.
Medina, R.
Rasmussen, M.
Fordyce, S.
Calvignac-Spencer, S.
Willerslev, E.
Gilbert, Thomas
Helgen, K.
Roca, A.
Greenwood, A.
author_facet Avila-Arcos, M.
Ho, S.
Ishida, Y.
Nikolaidis, N.
Tsangaras, K.
Honig, K.
Medina, R.
Rasmussen, M.
Fordyce, S.
Calvignac-Spencer, S.
Willerslev, E.
Gilbert, Thomas
Helgen, K.
Roca, A.
Greenwood, A.
author_sort Avila-Arcos, M.
building Curtin Institutional Repository
collection Online Access
description Although endogenous retroviruses are common across vertebrate genomes, the koala retrovirus (KoRV) is the only retrovirus known to be currently invading the germ line of its host. KoRV is believed to have first infected koalas in northern Australia less than two centuries ago. We examined KoRV in 28 koala museum skins collected in the late 19th and 20th centuries and deep sequenced the complete proviral envelope region from five northern Australian specimens. Strikingly, KoRV env sequences were conserved among koalas collected over the span of a century, and two functional motifs that affect viral infectivity were fixed across the museum koala specimens. We detected only 20 env polymorphisms among the koalas, likely representing derived mutations subject to purifying selection. Among northern Australian koalas, KoRV was already ubiquitous by the late 19th century, suggesting that KoRV evolved and spread among koala populations more slowly than previously believed. Given that museum and modern koalas share nearly identical KoRV sequences, it is likely that koala populations, for more than a century, have experienced increased susceptibility to diseases caused by viral pathogenesis.
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institution Curtin University Malaysia
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publishDate 2013
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spelling curtin-20.500.11937-123042017-02-28T01:34:05Z One Hundred Twenty Years of Koala Retrovirus Evolution Determined from Museum Skins Avila-Arcos, M. Ho, S. Ishida, Y. Nikolaidis, N. Tsangaras, K. Honig, K. Medina, R. Rasmussen, M. Fordyce, S. Calvignac-Spencer, S. Willerslev, E. Gilbert, Thomas Helgen, K. Roca, A. Greenwood, A. receptor-binding sequence protein leukemia-virus KoRV endogenous retroviruses ancient DNA genome Phascolarctos cinereus Although endogenous retroviruses are common across vertebrate genomes, the koala retrovirus (KoRV) is the only retrovirus known to be currently invading the germ line of its host. KoRV is believed to have first infected koalas in northern Australia less than two centuries ago. We examined KoRV in 28 koala museum skins collected in the late 19th and 20th centuries and deep sequenced the complete proviral envelope region from five northern Australian specimens. Strikingly, KoRV env sequences were conserved among koalas collected over the span of a century, and two functional motifs that affect viral infectivity were fixed across the museum koala specimens. We detected only 20 env polymorphisms among the koalas, likely representing derived mutations subject to purifying selection. Among northern Australian koalas, KoRV was already ubiquitous by the late 19th century, suggesting that KoRV evolved and spread among koala populations more slowly than previously believed. Given that museum and modern koalas share nearly identical KoRV sequences, it is likely that koala populations, for more than a century, have experienced increased susceptibility to diseases caused by viral pathogenesis. 2013 Journal Article http://hdl.handle.net/20.500.11937/12304 http://mbe.oxfordjournals.org/content/30/2/299.full.pdf+html?sid=fdfdecf9-8b7d-487f-94ed-82613915e5c5 Oxford Univ Press restricted
spellingShingle receptor-binding
sequence
protein
leukemia-virus
KoRV
endogenous retroviruses
ancient DNA
genome
Phascolarctos cinereus
Avila-Arcos, M.
Ho, S.
Ishida, Y.
Nikolaidis, N.
Tsangaras, K.
Honig, K.
Medina, R.
Rasmussen, M.
Fordyce, S.
Calvignac-Spencer, S.
Willerslev, E.
Gilbert, Thomas
Helgen, K.
Roca, A.
Greenwood, A.
One Hundred Twenty Years of Koala Retrovirus Evolution Determined from Museum Skins
title One Hundred Twenty Years of Koala Retrovirus Evolution Determined from Museum Skins
title_full One Hundred Twenty Years of Koala Retrovirus Evolution Determined from Museum Skins
title_fullStr One Hundred Twenty Years of Koala Retrovirus Evolution Determined from Museum Skins
title_full_unstemmed One Hundred Twenty Years of Koala Retrovirus Evolution Determined from Museum Skins
title_short One Hundred Twenty Years of Koala Retrovirus Evolution Determined from Museum Skins
title_sort one hundred twenty years of koala retrovirus evolution determined from museum skins
topic receptor-binding
sequence
protein
leukemia-virus
KoRV
endogenous retroviruses
ancient DNA
genome
Phascolarctos cinereus
url http://mbe.oxfordjournals.org/content/30/2/299.full.pdf+html?sid=fdfdecf9-8b7d-487f-94ed-82613915e5c5
http://hdl.handle.net/20.500.11937/12304