Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites

Microbially induced calcium carbonate precipitation (MICCP) is a naturally occurring biological process that has various applications in remediation and restoration of a range of building materials. In the present investigation, five ureolytic bacterial isolates capable of inducing calcium carbonate...

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Main Authors: Dhami, N., Reddy, M., Mukherjee, Abhijit
Format: Journal Article
Published: Han'gug Mi'saengmul Saengmyeong Gong Haghoe 2013
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/11583
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author Dhami, N.
Reddy, M.
Mukherjee, Abhijit
author_facet Dhami, N.
Reddy, M.
Mukherjee, Abhijit
author_sort Dhami, N.
building Curtin Institutional Repository
collection Online Access
description Microbially induced calcium carbonate precipitation (MICCP) is a naturally occurring biological process that has various applications in remediation and restoration of a range of building materials. In the present investigation, five ureolytic bacterial isolates capable of inducing calcium carbonate precipitation were isolated from calcareous soils on the basis of production of urease, carbonic anhydrase, extrapolymeric substances, and biofilm. Bacterial isolates were identified as Bacillus megaterium, B. cereus, B. thuringiensis, B. subtilis, and Lysinibacillus fusiformis based on 16S rRNA analysis. The calcium carbonate polymorphs produced by various bacterial isolates were analyzed by scanning electron microscopy, confocal laser scanning microscopy, X ray diffraction, and Fourier transmission infra red spectroscopy. A strainspecific precipitation of calcium carbonate forms was observed from different bacterial isolates. Based on the type of polymorph precipitated, the technology of MICCP can be applied for remediation of various building materials.
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format Journal Article
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institution Curtin University Malaysia
institution_category Local University
last_indexed 2025-11-14T06:55:37Z
publishDate 2013
publisher Han'gug Mi'saengmul Saengmyeong Gong Haghoe
recordtype eprints
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spelling curtin-20.500.11937-115832017-09-13T14:53:51Z Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites Dhami, N. Reddy, M. Mukherjee, Abhijit Vaterite Carbonic anhydrase Biofilm Calcite Urease Bacillus Microbially induced calcium carbonate precipitation (MICCP) is a naturally occurring biological process that has various applications in remediation and restoration of a range of building materials. In the present investigation, five ureolytic bacterial isolates capable of inducing calcium carbonate precipitation were isolated from calcareous soils on the basis of production of urease, carbonic anhydrase, extrapolymeric substances, and biofilm. Bacterial isolates were identified as Bacillus megaterium, B. cereus, B. thuringiensis, B. subtilis, and Lysinibacillus fusiformis based on 16S rRNA analysis. The calcium carbonate polymorphs produced by various bacterial isolates were analyzed by scanning electron microscopy, confocal laser scanning microscopy, X ray diffraction, and Fourier transmission infra red spectroscopy. A strainspecific precipitation of calcium carbonate forms was observed from different bacterial isolates. Based on the type of polymorph precipitated, the technology of MICCP can be applied for remediation of various building materials. 2013 Journal Article http://hdl.handle.net/20.500.11937/11583 10.4014/jmb.1212.11087 Han'gug Mi'saengmul Saengmyeong Gong Haghoe restricted
spellingShingle Vaterite
Carbonic anhydrase
Biofilm
Calcite
Urease
Bacillus
Dhami, N.
Reddy, M.
Mukherjee, Abhijit
Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites
title Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites
title_full Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites
title_fullStr Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites
title_full_unstemmed Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites
title_short Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites
title_sort biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites
topic Vaterite
Carbonic anhydrase
Biofilm
Calcite
Urease
Bacillus
url http://hdl.handle.net/20.500.11937/11583