Differential expression of the Bcl-2 and Bax isoforms in CD19 positive B-lymphocytes isolated from patients diagnosed with chronic lymphocytic leukaemia
Aim: To examine the relative gene expression levels of the anti-apoptotic Bcl-2a and ß isoforms and the pro-apoptotic Baxa and ß isoforms in patients with chronic lymphocytic leukaemia (CLL) and healthy controls (HC). Methods: Peripheral blood was obtained from 36 patients diagnosed with CLL and 10...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
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2012
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| Online Access: | http://hdl.handle.net/20.500.11937/11531 |
| _version_ | 1848747831304650752 |
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| author | Ghassemifar, Reza Forster, L. Finlayson, J. Calogero, T. Augustson, B. Joske, D. Cull, G. |
| author_facet | Ghassemifar, Reza Forster, L. Finlayson, J. Calogero, T. Augustson, B. Joske, D. Cull, G. |
| author_sort | Ghassemifar, Reza |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Aim: To examine the relative gene expression levels of the anti-apoptotic Bcl-2a and ß isoforms and the pro-apoptotic Baxa and ß isoforms in patients with chronic lymphocytic leukaemia (CLL) and healthy controls (HC). Methods: Peripheral blood was obtained from 36 patients diagnosed with CLL and 10 HC. CD19+ B-lymphocytes were isolated using an antibody coupled magnetic bead isolation system; from these cells the total RNA was isolated and purified. The relative levels of gene expression were examined by quantitative real-time polymerase chain reaction (qReTi-PCR) using primers specific for each isoform. Results: Bcl-2a and Baxa are expressed at higher levels than their ß-isoforms in CLL and HC. Bcl-2a, Bcl-2ß and Baxb expression is increased in CLL while Bax-a is expressed at similar levels to HC. The Bcl-2a/Bcl-2ß ratio is similar in CLL and HC. The Bcl-2a/Baxa ratio is increased in CLL when compared with HC. Conclusion: Bcl-2a and Baxa appear to be the dominant antiand pro-apoptotic isoforms in CLL. The Bcl-2a/Baxa ratio is increased in CLLwhile the Bcl-2a/Bcl-2ß ratio is similar to HC. |
| first_indexed | 2025-11-14T06:55:24Z |
| format | Journal Article |
| id | curtin-20.500.11937-11531 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:55:24Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-115312017-09-13T14:53:18Z Differential expression of the Bcl-2 and Bax isoforms in CD19 positive B-lymphocytes isolated from patients diagnosed with chronic lymphocytic leukaemia Ghassemifar, Reza Forster, L. Finlayson, J. Calogero, T. Augustson, B. Joske, D. Cull, G. Aim: To examine the relative gene expression levels of the anti-apoptotic Bcl-2a and ß isoforms and the pro-apoptotic Baxa and ß isoforms in patients with chronic lymphocytic leukaemia (CLL) and healthy controls (HC). Methods: Peripheral blood was obtained from 36 patients diagnosed with CLL and 10 HC. CD19+ B-lymphocytes were isolated using an antibody coupled magnetic bead isolation system; from these cells the total RNA was isolated and purified. The relative levels of gene expression were examined by quantitative real-time polymerase chain reaction (qReTi-PCR) using primers specific for each isoform. Results: Bcl-2a and Baxa are expressed at higher levels than their ß-isoforms in CLL and HC. Bcl-2a, Bcl-2ß and Baxb expression is increased in CLL while Bax-a is expressed at similar levels to HC. The Bcl-2a/Bcl-2ß ratio is similar in CLL and HC. The Bcl-2a/Baxa ratio is increased in CLL when compared with HC. Conclusion: Bcl-2a and Baxa appear to be the dominant antiand pro-apoptotic isoforms in CLL. The Bcl-2a/Baxa ratio is increased in CLLwhile the Bcl-2a/Bcl-2ß ratio is similar to HC. 2012 Journal Article http://hdl.handle.net/20.500.11937/11531 10.1097/PAT.0b013e32835a0142 restricted |
| spellingShingle | Ghassemifar, Reza Forster, L. Finlayson, J. Calogero, T. Augustson, B. Joske, D. Cull, G. Differential expression of the Bcl-2 and Bax isoforms in CD19 positive B-lymphocytes isolated from patients diagnosed with chronic lymphocytic leukaemia |
| title | Differential expression of the Bcl-2 and Bax isoforms in CD19 positive B-lymphocytes isolated from patients diagnosed with chronic lymphocytic leukaemia |
| title_full | Differential expression of the Bcl-2 and Bax isoforms in CD19 positive B-lymphocytes isolated from patients diagnosed with chronic lymphocytic leukaemia |
| title_fullStr | Differential expression of the Bcl-2 and Bax isoforms in CD19 positive B-lymphocytes isolated from patients diagnosed with chronic lymphocytic leukaemia |
| title_full_unstemmed | Differential expression of the Bcl-2 and Bax isoforms in CD19 positive B-lymphocytes isolated from patients diagnosed with chronic lymphocytic leukaemia |
| title_short | Differential expression of the Bcl-2 and Bax isoforms in CD19 positive B-lymphocytes isolated from patients diagnosed with chronic lymphocytic leukaemia |
| title_sort | differential expression of the bcl-2 and bax isoforms in cd19 positive b-lymphocytes isolated from patients diagnosed with chronic lymphocytic leukaemia |
| url | http://hdl.handle.net/20.500.11937/11531 |