Dissecting the role of G-protein signalling in primary metabolism in the wheat pathogen Stagonospora nodorum
Mutants of the wheat pathogenic fungus Stagonospora nodorum lacking G-protein subunits display a variety of phenotypes including melanization defects, primary metabolic changes and a decreased ability to sporulate. To better understand the causes of these phenotypes, Stagonospora nodorum strains lac...
| Main Authors: | , , , |
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| Format: | Journal Article |
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Society for General Microbiology
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/11514 |
| _version_ | 1848747826463375360 |
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| author | Gummer, J. Trengove, R. Oliver, Richard Solomon, P. |
| author_facet | Gummer, J. Trengove, R. Oliver, Richard Solomon, P. |
| author_sort | Gummer, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Mutants of the wheat pathogenic fungus Stagonospora nodorum lacking G-protein subunits display a variety of phenotypes including melanization defects, primary metabolic changes and a decreased ability to sporulate. To better understand the causes of these phenotypes, Stagonospora nodorum strains lacking a Gx, Gβ or Gγ subunit were compared to a wild-type strain using metabolomics. Agar plate growth at 22 °C revealed a number of fundamental metabolic changes and highlighted the influential role of these proteins in glucose utilization. A further characterization of the mutants was undertaken during prolonged storage at 4 °C, conditions known to induce sporulation in these sporulation-deficient signalling mutants. The abundance of several compounds positively correlated with the onset of sporulation including the dissacharide trehalose, the tryptophan degradation product tryptamine and the secondary metabolite alternariol; metabolites all previously associated with sporulation. Several other compounds decreased or were absent during sporulation. The levels of one such compound (Unknown_35.27_2194_319) decreased from being one of the more abundant compounds to absence during pycnidial maturation. This study has shed light on the role of G-protein subunits in primary metabolism during vegetative growth and exploited the cold-induced sporulation phenomenon in these mutants to identify some key metabolic changes that occur during asexual reproduction. |
| first_indexed | 2025-11-14T06:55:19Z |
| format | Journal Article |
| id | curtin-20.500.11937-11514 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:55:19Z |
| publishDate | 2013 |
| publisher | Society for General Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-115142017-09-13T14:53:53Z Dissecting the role of G-protein signalling in primary metabolism in the wheat pathogen Stagonospora nodorum Gummer, J. Trengove, R. Oliver, Richard Solomon, P. Mutants of the wheat pathogenic fungus Stagonospora nodorum lacking G-protein subunits display a variety of phenotypes including melanization defects, primary metabolic changes and a decreased ability to sporulate. To better understand the causes of these phenotypes, Stagonospora nodorum strains lacking a Gx, Gβ or Gγ subunit were compared to a wild-type strain using metabolomics. Agar plate growth at 22 °C revealed a number of fundamental metabolic changes and highlighted the influential role of these proteins in glucose utilization. A further characterization of the mutants was undertaken during prolonged storage at 4 °C, conditions known to induce sporulation in these sporulation-deficient signalling mutants. The abundance of several compounds positively correlated with the onset of sporulation including the dissacharide trehalose, the tryptophan degradation product tryptamine and the secondary metabolite alternariol; metabolites all previously associated with sporulation. Several other compounds decreased or were absent during sporulation. The levels of one such compound (Unknown_35.27_2194_319) decreased from being one of the more abundant compounds to absence during pycnidial maturation. This study has shed light on the role of G-protein subunits in primary metabolism during vegetative growth and exploited the cold-induced sporulation phenomenon in these mutants to identify some key metabolic changes that occur during asexual reproduction. 2013 Journal Article http://hdl.handle.net/20.500.11937/11514 10.1099/mic.0.067009-0 Society for General Microbiology fulltext |
| spellingShingle | Gummer, J. Trengove, R. Oliver, Richard Solomon, P. Dissecting the role of G-protein signalling in primary metabolism in the wheat pathogen Stagonospora nodorum |
| title | Dissecting the role of G-protein signalling in primary metabolism in the wheat pathogen Stagonospora nodorum |
| title_full | Dissecting the role of G-protein signalling in primary metabolism in the wheat pathogen Stagonospora nodorum |
| title_fullStr | Dissecting the role of G-protein signalling in primary metabolism in the wheat pathogen Stagonospora nodorum |
| title_full_unstemmed | Dissecting the role of G-protein signalling in primary metabolism in the wheat pathogen Stagonospora nodorum |
| title_short | Dissecting the role of G-protein signalling in primary metabolism in the wheat pathogen Stagonospora nodorum |
| title_sort | dissecting the role of g-protein signalling in primary metabolism in the wheat pathogen stagonospora nodorum |
| url | http://hdl.handle.net/20.500.11937/11514 |