Aptamer-Based Potentiometric Measurements of Proteins Using Ion-Selective Microelectrodes
We here report on the first example of an aptamer-based potentiometric sandwich assay of proteins. The measurements are based on CdS quantum dot labels of the secondary aptamer, which were determined with a novel solid-contact Cd2+-selective polymer membrane electrode after dissolution with hydrogen...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Published: |
American Chemical Society
2008
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| Online Access: | http://hdl.handle.net/20.500.11937/11209 |
| _version_ | 1848747744089341952 |
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| author | Numnuam, A. Chumbimuni-Torres, K. Xiang, Y. Bash, R. Thavarungkul, P. Kanatharana, P. Pretsch, E. Wang, J. Bakker, Eric |
| author_facet | Numnuam, A. Chumbimuni-Torres, K. Xiang, Y. Bash, R. Thavarungkul, P. Kanatharana, P. Pretsch, E. Wang, J. Bakker, Eric |
| author_sort | Numnuam, A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | We here report on the first example of an aptamer-based potentiometric sandwich assay of proteins. The measurements are based on CdS quantum dot labels of the secondary aptamer, which were determined with a novel solid-contact Cd2+-selective polymer membrane electrode after dissolution with hydrogen peroxide. The electrode exhibited cadmium ion detection limits of 100 pM in 100 mL samples and of 1 nM in 200 microwells, using a calcium-selective electrode as a pseudoreference electrode. As a prototype example, thrombin was measured in 200 samples with a lower detection limit of 0.14 nM corresponding to 28 fmol of analyte. The results show great promise for the potentiometric determination of proteins at very low concentrations in microliter samples. |
| first_indexed | 2025-11-14T06:54:00Z |
| format | Journal Article |
| id | curtin-20.500.11937-11209 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:54:00Z |
| publishDate | 2008 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-112092018-03-29T09:05:57Z Aptamer-Based Potentiometric Measurements of Proteins Using Ion-Selective Microelectrodes Numnuam, A. Chumbimuni-Torres, K. Xiang, Y. Bash, R. Thavarungkul, P. Kanatharana, P. Pretsch, E. Wang, J. Bakker, Eric We here report on the first example of an aptamer-based potentiometric sandwich assay of proteins. The measurements are based on CdS quantum dot labels of the secondary aptamer, which were determined with a novel solid-contact Cd2+-selective polymer membrane electrode after dissolution with hydrogen peroxide. The electrode exhibited cadmium ion detection limits of 100 pM in 100 mL samples and of 1 nM in 200 microwells, using a calcium-selective electrode as a pseudoreference electrode. As a prototype example, thrombin was measured in 200 samples with a lower detection limit of 0.14 nM corresponding to 28 fmol of analyte. The results show great promise for the potentiometric determination of proteins at very low concentrations in microliter samples. 2008 Journal Article http://hdl.handle.net/20.500.11937/11209 10.1021/ac701910r American Chemical Society restricted |
| spellingShingle | Numnuam, A. Chumbimuni-Torres, K. Xiang, Y. Bash, R. Thavarungkul, P. Kanatharana, P. Pretsch, E. Wang, J. Bakker, Eric Aptamer-Based Potentiometric Measurements of Proteins Using Ion-Selective Microelectrodes |
| title | Aptamer-Based Potentiometric Measurements of Proteins Using Ion-Selective Microelectrodes |
| title_full | Aptamer-Based Potentiometric Measurements of Proteins Using Ion-Selective Microelectrodes |
| title_fullStr | Aptamer-Based Potentiometric Measurements of Proteins Using Ion-Selective Microelectrodes |
| title_full_unstemmed | Aptamer-Based Potentiometric Measurements of Proteins Using Ion-Selective Microelectrodes |
| title_short | Aptamer-Based Potentiometric Measurements of Proteins Using Ion-Selective Microelectrodes |
| title_sort | aptamer-based potentiometric measurements of proteins using ion-selective microelectrodes |
| url | http://hdl.handle.net/20.500.11937/11209 |