Dietary quercetin attenuates oxidant-induced endothelial dysfunction and atherosclerosis in apolipoprotein e knockout mice fed a high-fat diet: A critical role for heme oxygenase-1
Several lines of evidence indicate that quercetin, a polyphenol derived in the diet from fruit and vegetables, contributes to cardiovascular health. We aimed to investigate the effects of dietary quercetin on endothelial function and atherosclerosis in mice fed a high-fat diet. Wild-type C57BL/6 (WT...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
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2013
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| Online Access: | http://hdl.handle.net/20.500.11937/10995 |
| _version_ | 1848747686410321920 |
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| author | Shen, Y. Ward, Natalie Hodgson, J. Puddey, I. Wang, Y. Zhang, D. Maghzal, G. Stocker, R. Croft, K. |
| author_facet | Shen, Y. Ward, Natalie Hodgson, J. Puddey, I. Wang, Y. Zhang, D. Maghzal, G. Stocker, R. Croft, K. |
| author_sort | Shen, Y. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Several lines of evidence indicate that quercetin, a polyphenol derived in the diet from fruit and vegetables, contributes to cardiovascular health. We aimed to investigate the effects of dietary quercetin on endothelial function and atherosclerosis in mice fed a high-fat diet. Wild-type C57BL/6 (WT) and apolipoprotein E gene knockout (ApoE−/−) mice were fed: (i) a high-fat diet (HFD) or (ii) a HFD supplemented with 0.05% w/w quercetin (HFD+Q), for 14 weeks. Compared with animals fed HFD, HFD+Q attenuated atherosclerosis in ApoE−/− mice. Treatment with the HFD+Q significantly improved endothelium-dependent relaxation of aortic rings isolated from WT but not ApoE−/− mice and attenuated hypochlorous acid-induced endothelial dysfunction in aortic rings of both WT and ApoE−/− mice. Mechanistic studies revealed that HFD+Q significantly improved plasma F2-isoprostanes, 24 h urinary nitrite, and endothelial nitric oxide synthase activity, and increased heme oxygenase-1 (HO-1) protein expression in the aortas of both WT and ApoE−/− mice (P<0.05). HFD+Q also resulted in small changes in plasma cholesterol (P<0.05 in WT) and plasma triacylglycerols (P<0.05 in ApoE −/−mice). In a separate experiment, quercetin did not protect against hypochlorite-induced endothelial dysfunction in arteries obtained from heterozygous HO-1 gene knockout mice with low expression of HO-1 protein. Quercetin protects mice fed a HFD against oxidant-induced endothelial dysfunction and ApoE−/− mice against atherosclerosis. These effects are associated with improvements in nitric oxide bioavailability and are critically related to arterial induction of HO-1. |
| first_indexed | 2025-11-14T06:53:05Z |
| format | Journal Article |
| id | curtin-20.500.11937-10995 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:53:05Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-109952017-09-13T14:54:24Z Dietary quercetin attenuates oxidant-induced endothelial dysfunction and atherosclerosis in apolipoprotein e knockout mice fed a high-fat diet: A critical role for heme oxygenase-1 Shen, Y. Ward, Natalie Hodgson, J. Puddey, I. Wang, Y. Zhang, D. Maghzal, G. Stocker, R. Croft, K. Several lines of evidence indicate that quercetin, a polyphenol derived in the diet from fruit and vegetables, contributes to cardiovascular health. We aimed to investigate the effects of dietary quercetin on endothelial function and atherosclerosis in mice fed a high-fat diet. Wild-type C57BL/6 (WT) and apolipoprotein E gene knockout (ApoE−/−) mice were fed: (i) a high-fat diet (HFD) or (ii) a HFD supplemented with 0.05% w/w quercetin (HFD+Q), for 14 weeks. Compared with animals fed HFD, HFD+Q attenuated atherosclerosis in ApoE−/− mice. Treatment with the HFD+Q significantly improved endothelium-dependent relaxation of aortic rings isolated from WT but not ApoE−/− mice and attenuated hypochlorous acid-induced endothelial dysfunction in aortic rings of both WT and ApoE−/− mice. Mechanistic studies revealed that HFD+Q significantly improved plasma F2-isoprostanes, 24 h urinary nitrite, and endothelial nitric oxide synthase activity, and increased heme oxygenase-1 (HO-1) protein expression in the aortas of both WT and ApoE−/− mice (P<0.05). HFD+Q also resulted in small changes in plasma cholesterol (P<0.05 in WT) and plasma triacylglycerols (P<0.05 in ApoE −/−mice). In a separate experiment, quercetin did not protect against hypochlorite-induced endothelial dysfunction in arteries obtained from heterozygous HO-1 gene knockout mice with low expression of HO-1 protein. Quercetin protects mice fed a HFD against oxidant-induced endothelial dysfunction and ApoE−/− mice against atherosclerosis. These effects are associated with improvements in nitric oxide bioavailability and are critically related to arterial induction of HO-1. 2013 Journal Article http://hdl.handle.net/20.500.11937/10995 10.1016/j.freeradbiomed.2013.08.185 restricted |
| spellingShingle | Shen, Y. Ward, Natalie Hodgson, J. Puddey, I. Wang, Y. Zhang, D. Maghzal, G. Stocker, R. Croft, K. Dietary quercetin attenuates oxidant-induced endothelial dysfunction and atherosclerosis in apolipoprotein e knockout mice fed a high-fat diet: A critical role for heme oxygenase-1 |
| title | Dietary quercetin attenuates oxidant-induced endothelial dysfunction and atherosclerosis in apolipoprotein e knockout mice fed a high-fat diet: A critical role for heme oxygenase-1 |
| title_full | Dietary quercetin attenuates oxidant-induced endothelial dysfunction and atherosclerosis in apolipoprotein e knockout mice fed a high-fat diet: A critical role for heme oxygenase-1 |
| title_fullStr | Dietary quercetin attenuates oxidant-induced endothelial dysfunction and atherosclerosis in apolipoprotein e knockout mice fed a high-fat diet: A critical role for heme oxygenase-1 |
| title_full_unstemmed | Dietary quercetin attenuates oxidant-induced endothelial dysfunction and atherosclerosis in apolipoprotein e knockout mice fed a high-fat diet: A critical role for heme oxygenase-1 |
| title_short | Dietary quercetin attenuates oxidant-induced endothelial dysfunction and atherosclerosis in apolipoprotein e knockout mice fed a high-fat diet: A critical role for heme oxygenase-1 |
| title_sort | dietary quercetin attenuates oxidant-induced endothelial dysfunction and atherosclerosis in apolipoprotein e knockout mice fed a high-fat diet: a critical role for heme oxygenase-1 |
| url | http://hdl.handle.net/20.500.11937/10995 |