Evaluation of a modified carbon micromesh electrode as a new substrate for electrochemical immunosensing
Here we report a novel immunosensor platform technology based on self-assembled monolayer (SAM) of electrodeposited gold (Au) nanostructures with subsequent antibody attachment. Carbon cloth micro-fibres were employed for the first time as a working electrode in an immunosensor device. Uniform distr...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
2011
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| Online Access: | http://hdl.handle.net/20.500.11937/10709 |
| _version_ | 1848747607848910848 |
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| author | Rathod, D. Warren, S. Keane, Kevin Egan, D. Dempsey, E. |
| author_facet | Rathod, D. Warren, S. Keane, Kevin Egan, D. Dempsey, E. |
| author_sort | Rathod, D. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Here we report a novel immunosensor platform technology based on self-assembled monolayer (SAM) of electrodeposited gold (Au) nanostructures with subsequent antibody attachment. Carbon cloth micro-fibres were employed for the first time as a working electrode in an immunosensor device. Uniform distribution of Au nanoparticles was achieved on the surface of microfibers with regulation of particle size and shape made possible via controlled scan rate during deposition from aqueous AuCl4. The electrodes were successfully characterised using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA). Chemiluminescence detection confirmed successful attachment of anti-actin antibodies onto the modified conducting surface, allowing detection of various concentrations of the actin antigen (0.1-1 g ml-1). The binding of antigen to antibody was successfully measured using EIS in 1 mM K3Fe(CN)6/K 4Fe(CN)6 as a redox probe, which allowed determination of charge transfer resistance (Rct) changes upon binding. A linear increase in Rct was observed with increasing concentration of antigen (actin). Therefore, we have clearly demonstrated that this new electrochemical immunosensor design is effective, and can be applied to the analysis of a clinically significant protein. © 2011 The Royal Society of Chemistry. |
| first_indexed | 2025-11-14T06:51:51Z |
| format | Journal Article |
| id | curtin-20.500.11937-10709 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:51:51Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-107092017-09-13T14:53:18Z Evaluation of a modified carbon micromesh electrode as a new substrate for electrochemical immunosensing Rathod, D. Warren, S. Keane, Kevin Egan, D. Dempsey, E. Here we report a novel immunosensor platform technology based on self-assembled monolayer (SAM) of electrodeposited gold (Au) nanostructures with subsequent antibody attachment. Carbon cloth micro-fibres were employed for the first time as a working electrode in an immunosensor device. Uniform distribution of Au nanoparticles was achieved on the surface of microfibers with regulation of particle size and shape made possible via controlled scan rate during deposition from aqueous AuCl4. The electrodes were successfully characterised using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA). Chemiluminescence detection confirmed successful attachment of anti-actin antibodies onto the modified conducting surface, allowing detection of various concentrations of the actin antigen (0.1-1 g ml-1). The binding of antigen to antibody was successfully measured using EIS in 1 mM K3Fe(CN)6/K 4Fe(CN)6 as a redox probe, which allowed determination of charge transfer resistance (Rct) changes upon binding. A linear increase in Rct was observed with increasing concentration of antigen (actin). Therefore, we have clearly demonstrated that this new electrochemical immunosensor design is effective, and can be applied to the analysis of a clinically significant protein. © 2011 The Royal Society of Chemistry. 2011 Journal Article http://hdl.handle.net/20.500.11937/10709 10.1039/c0ay00665c restricted |
| spellingShingle | Rathod, D. Warren, S. Keane, Kevin Egan, D. Dempsey, E. Evaluation of a modified carbon micromesh electrode as a new substrate for electrochemical immunosensing |
| title | Evaluation of a modified carbon micromesh electrode as a new substrate for electrochemical immunosensing |
| title_full | Evaluation of a modified carbon micromesh electrode as a new substrate for electrochemical immunosensing |
| title_fullStr | Evaluation of a modified carbon micromesh electrode as a new substrate for electrochemical immunosensing |
| title_full_unstemmed | Evaluation of a modified carbon micromesh electrode as a new substrate for electrochemical immunosensing |
| title_short | Evaluation of a modified carbon micromesh electrode as a new substrate for electrochemical immunosensing |
| title_sort | evaluation of a modified carbon micromesh electrode as a new substrate for electrochemical immunosensing |
| url | http://hdl.handle.net/20.500.11937/10709 |