Insulinotropic properties of whey protein hydrolysates and impact of peptide fractionation on insulinotropic response
The influence of different substrates and the effect of pH regulation during enzymatic hydrolysis of whey protein on glucose-stimulated insulin secretion by BRIN-BD11 pancreatic beta cells were studied. No significant differences (P ≥ 0.05) were detected in terms of glucose-stimulated insulin secret...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Published: |
Elsevier
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/10693 |
| _version_ | 1848747603347374080 |
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| author | Nongonierma, Alice Gaudel, Celine Murray, Brian Flynn, Sarah Kelly, Phillip Newsholme, Philip FitzGerald, Richard |
| author_facet | Nongonierma, Alice Gaudel, Celine Murray, Brian Flynn, Sarah Kelly, Phillip Newsholme, Philip FitzGerald, Richard |
| author_sort | Nongonierma, Alice |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The influence of different substrates and the effect of pH regulation during enzymatic hydrolysis of whey protein on glucose-stimulated insulin secretion by BRIN-BD11 pancreatic beta cells were studied. No significant differences (P ≥ 0.05) were detected in terms of glucose-stimulated insulin secretion by BRIN-BD11 pancreatic beta cells exposed to whey protein hydrolysates (WPH1 and WPH2) obtained with two different whey protein substrates. The whey protein hydrolysate (WPH3) obtained without pH regulation during hydrolysis, had a significantly lower insulinotropic potential in BRIN-BD11 cells than the WPH1 hydrolysate that was manufactured with pH regulation. Fractionation of WPH1 was carried out to enrich bioactive peptides. Comparing the different fractionation techniques studied (solid-phase extraction and semi-preparative reverse phase-high performance liquid chromatography), the most potent insulinotropic fraction was enriched in free amino acids and contained relatively hydrophilic peptides. This indicates that amino acids and hydrophilic peptides may be involved in the insulinotropic effect of WPH1. |
| first_indexed | 2025-11-14T06:51:46Z |
| format | Journal Article |
| id | curtin-20.500.11937-10693 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:51:46Z |
| publishDate | 2013 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-106932018-03-29T09:05:57Z Insulinotropic properties of whey protein hydrolysates and impact of peptide fractionation on insulinotropic response Nongonierma, Alice Gaudel, Celine Murray, Brian Flynn, Sarah Kelly, Phillip Newsholme, Philip FitzGerald, Richard The influence of different substrates and the effect of pH regulation during enzymatic hydrolysis of whey protein on glucose-stimulated insulin secretion by BRIN-BD11 pancreatic beta cells were studied. No significant differences (P ≥ 0.05) were detected in terms of glucose-stimulated insulin secretion by BRIN-BD11 pancreatic beta cells exposed to whey protein hydrolysates (WPH1 and WPH2) obtained with two different whey protein substrates. The whey protein hydrolysate (WPH3) obtained without pH regulation during hydrolysis, had a significantly lower insulinotropic potential in BRIN-BD11 cells than the WPH1 hydrolysate that was manufactured with pH regulation. Fractionation of WPH1 was carried out to enrich bioactive peptides. Comparing the different fractionation techniques studied (solid-phase extraction and semi-preparative reverse phase-high performance liquid chromatography), the most potent insulinotropic fraction was enriched in free amino acids and contained relatively hydrophilic peptides. This indicates that amino acids and hydrophilic peptides may be involved in the insulinotropic effect of WPH1. 2013 Journal Article http://hdl.handle.net/20.500.11937/10693 10.1016/j.idairyj.2013.05.014 Elsevier restricted |
| spellingShingle | Nongonierma, Alice Gaudel, Celine Murray, Brian Flynn, Sarah Kelly, Phillip Newsholme, Philip FitzGerald, Richard Insulinotropic properties of whey protein hydrolysates and impact of peptide fractionation on insulinotropic response |
| title | Insulinotropic properties of whey protein hydrolysates and impact of peptide fractionation on insulinotropic response |
| title_full | Insulinotropic properties of whey protein hydrolysates and impact of peptide fractionation on insulinotropic response |
| title_fullStr | Insulinotropic properties of whey protein hydrolysates and impact of peptide fractionation on insulinotropic response |
| title_full_unstemmed | Insulinotropic properties of whey protein hydrolysates and impact of peptide fractionation on insulinotropic response |
| title_short | Insulinotropic properties of whey protein hydrolysates and impact of peptide fractionation on insulinotropic response |
| title_sort | insulinotropic properties of whey protein hydrolysates and impact of peptide fractionation on insulinotropic response |
| url | http://hdl.handle.net/20.500.11937/10693 |