Transformation of Fulvia fulva, a fungal pathogen of tomato to Hygromycin B resistance
A transformation system for the tomato pathogen Fulvia fulva has been developed. Hygromycin B resistant colonies were obtained after treatment of protoplasts with a plasmid containing an E. coli hygromycin B phosphotransferase gene fused to an Aspergillus nidulans promoter. The DNA was stably integr...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
1987
|
| Online Access: | http://hdl.handle.net/20.500.11937/10267 |
| _version_ | 1848746185136799744 |
|---|---|
| author | Oliver, Richard Roberts, I. Harling, R. Kenyon, L. Punt, P. Dingemanse, M. Van Den Hondel, C. |
| author_facet | Oliver, Richard Roberts, I. Harling, R. Kenyon, L. Punt, P. Dingemanse, M. Van Den Hondel, C. |
| author_sort | Oliver, Richard |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | A transformation system for the tomato pathogen Fulvia fulva has been developed. Hygromycin B resistant colonies were obtained after treatment of protoplasts with a plasmid containing an E. coli hygromycin B phosphotransferase gene fused to an Aspergillus nidulans promoter. The DNA was stably integrated into the genome. The number and sites of integrations varied among transformants. The demonstration of transformation opens the way for the molecular genetic analysis of the interaction of Fulvia with tomato. |
| first_indexed | 2025-11-14T06:29:14Z |
| format | Journal Article |
| id | curtin-20.500.11937-10267 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:29:14Z |
| publishDate | 1987 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-102672017-09-13T16:07:06Z Transformation of Fulvia fulva, a fungal pathogen of tomato to Hygromycin B resistance Oliver, Richard Roberts, I. Harling, R. Kenyon, L. Punt, P. Dingemanse, M. Van Den Hondel, C. A transformation system for the tomato pathogen Fulvia fulva has been developed. Hygromycin B resistant colonies were obtained after treatment of protoplasts with a plasmid containing an E. coli hygromycin B phosphotransferase gene fused to an Aspergillus nidulans promoter. The DNA was stably integrated into the genome. The number and sites of integrations varied among transformants. The demonstration of transformation opens the way for the molecular genetic analysis of the interaction of Fulvia with tomato. 1987 Journal Article http://hdl.handle.net/20.500.11937/10267 10.1007/BF00436885 restricted |
| spellingShingle | Oliver, Richard Roberts, I. Harling, R. Kenyon, L. Punt, P. Dingemanse, M. Van Den Hondel, C. Transformation of Fulvia fulva, a fungal pathogen of tomato to Hygromycin B resistance |
| title | Transformation of Fulvia fulva, a fungal pathogen of tomato to Hygromycin B resistance |
| title_full | Transformation of Fulvia fulva, a fungal pathogen of tomato to Hygromycin B resistance |
| title_fullStr | Transformation of Fulvia fulva, a fungal pathogen of tomato to Hygromycin B resistance |
| title_full_unstemmed | Transformation of Fulvia fulva, a fungal pathogen of tomato to Hygromycin B resistance |
| title_short | Transformation of Fulvia fulva, a fungal pathogen of tomato to Hygromycin B resistance |
| title_sort | transformation of fulvia fulva, a fungal pathogen of tomato to hygromycin b resistance |
| url | http://hdl.handle.net/20.500.11937/10267 |