Bioanalytical evaluation of Lactobacillus delbrueckii subsp. lactis 313 cell-envelope proteinase extraction
Lactobacilli cell-envelope proteinases (CEPs) have demonstrated numerous biopharmaceutical applications in the development of new streams of blockbuster nutraceuticals; thus, the development of efficient and commercially viable methods for CEP extraction will promote their full-scale application. In...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
Pergamon
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/10101 |
| _version_ | 1848746139478654976 |
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| author | Agyei, D. Lim, W. Zass, M. Tan, D. Danquah, Michael |
| author_facet | Agyei, D. Lim, W. Zass, M. Tan, D. Danquah, Michael |
| author_sort | Agyei, D. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Lactobacilli cell-envelope proteinases (CEPs) have demonstrated numerous biopharmaceutical applications in the development of new streams of blockbuster nutraceuticals; thus, the development of efficient and commercially viable methods for CEP extraction will promote their full-scale application. In this study, the sub-cellular location of CEPs in Lactobacillus delbrueckii subsp. lactis 313 (LDL 313) was identified and the effects of different extraction methods were investigated for their ability to efficiently release CEPs from LDL 313. Significantly high relative proteinase activity of~95% was detected in cell-wall fractions and ~5% activity was observed for osmotic fluids, implying that proteinases in LDL 313 are cell-wall bound. CEPs were released from cell-wall via incubation in calcium-free buffer, indicating the enzyme is liable to self-digestion and ionic misfolding. Of the different extraction methods investigated, the use of 5. M LiCl was the most suitable, under the conditions of experimentation, for releasing high levels of CEPs from LDL 313. |
| first_indexed | 2025-11-14T06:28:30Z |
| format | Journal Article |
| id | curtin-20.500.11937-10101 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:28:30Z |
| publishDate | 2013 |
| publisher | Pergamon |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-101012017-09-13T14:50:14Z Bioanalytical evaluation of Lactobacillus delbrueckii subsp. lactis 313 cell-envelope proteinase extraction Agyei, D. Lim, W. Zass, M. Tan, D. Danquah, Michael Lactobacilli cell-envelope proteinases (CEPs) have demonstrated numerous biopharmaceutical applications in the development of new streams of blockbuster nutraceuticals; thus, the development of efficient and commercially viable methods for CEP extraction will promote their full-scale application. In this study, the sub-cellular location of CEPs in Lactobacillus delbrueckii subsp. lactis 313 (LDL 313) was identified and the effects of different extraction methods were investigated for their ability to efficiently release CEPs from LDL 313. Significantly high relative proteinase activity of~95% was detected in cell-wall fractions and ~5% activity was observed for osmotic fluids, implying that proteinases in LDL 313 are cell-wall bound. CEPs were released from cell-wall via incubation in calcium-free buffer, indicating the enzyme is liable to self-digestion and ionic misfolding. Of the different extraction methods investigated, the use of 5. M LiCl was the most suitable, under the conditions of experimentation, for releasing high levels of CEPs from LDL 313. 2013 Journal Article http://hdl.handle.net/20.500.11937/10101 10.1016/j.ces.2013.03.049 Pergamon restricted |
| spellingShingle | Agyei, D. Lim, W. Zass, M. Tan, D. Danquah, Michael Bioanalytical evaluation of Lactobacillus delbrueckii subsp. lactis 313 cell-envelope proteinase extraction |
| title | Bioanalytical evaluation of Lactobacillus delbrueckii subsp. lactis 313 cell-envelope proteinase extraction |
| title_full | Bioanalytical evaluation of Lactobacillus delbrueckii subsp. lactis 313 cell-envelope proteinase extraction |
| title_fullStr | Bioanalytical evaluation of Lactobacillus delbrueckii subsp. lactis 313 cell-envelope proteinase extraction |
| title_full_unstemmed | Bioanalytical evaluation of Lactobacillus delbrueckii subsp. lactis 313 cell-envelope proteinase extraction |
| title_short | Bioanalytical evaluation of Lactobacillus delbrueckii subsp. lactis 313 cell-envelope proteinase extraction |
| title_sort | bioanalytical evaluation of lactobacillus delbrueckii subsp. lactis 313 cell-envelope proteinase extraction |
| url | http://hdl.handle.net/20.500.11937/10101 |