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1860799968610091008
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INTELEK Repository
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Online Access
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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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2013-01-08 15:41:38
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Restricted Document
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| id |
8117
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UniSZA
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Ahmad, N. S., Khalid, B. A. K., Luke D. A. and Nirwana, I. 2005. Tocotrienol offers better protection than tocopherol from free radical induced damaged of rat bone. Clinical Experimental of Phamacology and Physiology 32: 761-770. Aggarwal, B., Sundaram, C., Prasad S. and Kannapan, R. 2010. Tocotrienols, the vitamin E of the 21st century: Its potential against cancer and other chronic diseases. Biochemical Pharmacology 80: 1613-1631. Azzi, A., Aratri, E., Boscoboinik, D., Clement, S., Ozer, N. K, Ricciarelli, R. and Spycher S. 1998. Molecular basis of α-tocopherol control of smooth muscle cell proliferation. Biofactors 7: 314. Berliner, J. A. and Heinecke, J. W. 1996. The role of oxidized lipoproteins in atherogenesis. Free Radical Biology and Medicine 20 (5): 707-727. Berliner, J. A., Navab, M., Fogelman, A. M., Frank, J. S., Demer, L. L., Edwards, P. A., Watson, A. D. and Lusis, A. J. 1995. Atherosclerosis: basic mechanisms. Oxidation, inflammation, and genetics. Circulation 91: 2488-2496. Brand, K., Page, S., Rogler, G., Bartsch, A., Brandl, R., Knuechel, R., Page, M., Kaltschmidt, C., Baeuerle, P. A. and Neumeier D. 1996. Activated transcription factor NF-kappa B is present in the atherosclerotic lesion. Journal of Clinical Investigations 97: 1715- 1722. Collins, T. 1993. Endothelial NF-kappaB and the initiation of the atherosclerotic lesions. Laboratory Investigations 68 : 499-506. Das, S., Powell, S. R., Wang, P., Divald, K., Nasaretnam, K. and Tosak, A. 2005. Cardioprotection with palm oil: antioxidant activity of tocotrienol linked with its ability to stabilize proteosomes. American Journal of Physiology. Heart and Circulatory Physiology 289: 361-367. Gee, P. T. 2011. Unleashing the untold and misunderstood observations of vitamin E. Genes Nutrition 6: 5-16. Gosling, J., Slaymaker, S., Gu, L., Tseng, S., Zlot, C. H., Young, S. G., Rollins, B. J. and Charo I. F. 1999. MCP-1 deficiency reduces susceptibility to atherosclerosis in mice that overexpress human apolipoprotein B. Journal of Clinical Investigations 103: 773-778. Gulcin, I., Mshvildadze, V., Gepdiremen, A. and Elias, R. 2006. Screening of antiradical and antioxidant activity of monodesmosides and crude extract from Leontice smirnowii tuber. Phytomedicine 13: 343-351. Kamat, J. P. and Devasagayamm, T. P. A., 1995. Tocotrienols from palm oil as potent inhibitors of lipid peroxidation and protein oxidation in rat brain mitochondria. Neuroscience letters 195: 179-182. Keaney, J. F. Jr., Gaziano, G. M., Xu, A., Frei, B., Curran Celantano, J., Shwaery, G. T., Loscalzo, J. and Vita, J. A. 1994. Low dose α-tocopherol improves and high dose α-tocopherol worsens endothelial vasodilators function in cholesterol fed rabbit. Clinical Investigations 93: 884-851. Kim, J. S. 2005. Radical scavenging capacity and antioxidant activity of the E vitamer fraction in rice bran. Journal of Food Science 70 (Nr. 3): 208-213. Lee, J. Y., Hwang, W. I. and Lim, S. T. 2004. Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. Journal of Ethnopharmacology 93: 409-415. Li, D. Y., Chen, H. J. and Mehta, J. L. 2001. Statins inhibit oxidized-LDL-mediated LOX-1 expression, uptake of oxidized-LDL and reduction in PKB phosphorylation. Cardiovascular Research 52: 130-135. Martin, A., Foxall, T., Blumberg, J. B. and Meydani, M. 1997. Vitamin E inhibits low density lipoprotein induced adhesion of monocytes to human aortic endothelial cells in vitro. Arteriosclerosis, Thrombosis and Vascular Biology 17:429-436. Miller, E. R., Barriuso, R. P., Dalal, D., Riemersma, R. A., Appel, L. J. and Guallar, E. 2005. Meta-analysis: High dosage vitamin E supplementation may increase all-cause mortality. Annals of Internal Medicine 142: 37-46. Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65(1-2): 55-63. Osawa, T. and Namiki, M. 1981. A novel type of antioxidant isolated from leaf wax of Eucalyptus leaves. Agricultural & Biological Chemistry 45(3): 735-739. Palozza, P., Verdecchia, S., Avanzi, L., Vertuani, S., Serini, S., Lannone, A. and Manfredini, S. 2006. Comparative antioxidant activity of tocotrienols and the novel chromanyl-polyisoprenyl molecule FeAox-6 in isolated membranes and intact cells. Molecular and Cellular Biochemistry 287: 21-32. Poolman, T. M., Nga, L. L., Farmer, P. B. and Manson, M. M. 2005. Inhibition of the respiratory burst by resveratrol in human monocytes: Correlation with inhibition of PI3K signaling. Free Radical Biology and Medicine 39: 118 -132. Qureshi, A. A., Bradlow, B. A., Brace, L., Mangello, J., Peterson, D. M., Pearce, B. C., Wright. J. J., Gapor, A. and Elson, C. E. 1995. Response of hypercholesterolaemic subjects to administration of tocotrienols. Lipids 30(12): 1171-1177. Ross, R. 1999. Atherosclerosis: an inflammatory disease. New England Journal of Medicine 340: 115-126. Sen, K. S., Khanna, S. and Roy, S. 2006. Tocotrienols: Vitamin E beyond tocopherols. Life Sciences 78: 2088-2098. Serbinova, E., Kagan, V., Han, D. and Packer, L. 1991. Free radical recycling and intramembrane mobility in the antioxidant properties of α-Tocopherol and α-Tocotrienol. Free Radical Biology and Medicine 10: 263-75. Soelaiman, I. N., Ahmad, N. S. and Khalid, B. A. K. 2004. Palm oil tocotrienol mixture better than α-tocopherol acetate in protecting bones against free – radical induced elevation of bone – resorbing cytokines. Asia Pacific Journal of Clinical Nutrition 13 (Suppl): S111. Tan, B. 2010. Tocotrienols: The New Vitamin E. Downloaded from http: www. Spacedoc.net on 11/01/2012. Theriault, A., Chao, T. C. and Gapor, A. 2002. Atherosclerosis 160: 21-30. Tribble, D. L. 1999. AHA Science Advisory. Antioxidant consumption and risk of coronary heart disease: emphasis on vitamin C, vitamin E and beta carotene: a statement from for healthcare professionals from the American Heart Association. Circulation 99: 591- 595. Vivekanathan, D. P., Penn, M. S., Sapp, S. H., Hsu, A. and Topol, E. J. 2003. Use of antioxidant vitamins for the prevention of cardiovascular disease:meta-analysis of randomised trials. Lancet 361: 2017-2023. Wang, H. and Joseph, J. A. 1999. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radical Biology and Medicine 27: 612-616.
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3990-01-FH02-FPBSM-14-00548.pdf
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=8117
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8117 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=8117 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 8 1.6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in Adobe InDesign CS3 (5.0) 2013-01-08 15:41:38 adobe:docid:indd:0afce222-550e-11e2-bccf-d6b1b48dc0a5 3990-01-FH02-FPBSM-14-00548.pdf UniSZA Private Access Optimal antioxidant activity with moderate concentrations of Tocotrienol rich fraction (TRF) in in vitro assays International Food Research Journal Vitamin E is known to have potent antioxidant activity and plays an important role in reducing oxidative stress, a pivotal step in atherogenesis. However, several randomised clinical trials using α-tocopherol have failed to demonstrate consistent beneficial effects of antioxidants against atherosclerosis and clinical endpoints. Tocotrienol, a vitamin E compound analogue is shown to have more potent antioxidant activity compared to tocopherol. Finding the optimal anti-oxidative dose is crucial and may effectively be applied for cardioprotection in human. The objective of this study was to determine the optimal dose of tocotrienol rich fraction (TRF) with highest antioxidant activity in vitro using the ferric thiocyanate (FTC), 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity and 2’, 7’- dichlorofluorescein diacetate (DCFHDA) assays. It was found that TRF exhibited potent antioxidant and free radical scavenging activities with an IC50 of 22.10 + 0.01 µg/ml. In all assays, TRF had optimal antioxidant activity at moderate concentrations (10-100 µg/ml). In conclusion, TRF has potent antioxidant activity, which is optimal at moderate concentrations. 687-694 Ahmad, N. S., Khalid, B. A. K., Luke D. A. and Nirwana, I. 2005. Tocotrienol offers better protection than tocopherol from free radical induced damaged of rat bone. Clinical Experimental of Phamacology and Physiology 32: 761-770. Aggarwal, B., Sundaram, C., Prasad S. and Kannapan, R. 2010. Tocotrienols, the vitamin E of the 21st century: Its potential against cancer and other chronic diseases. Biochemical Pharmacology 80: 1613-1631. Azzi, A., Aratri, E., Boscoboinik, D., Clement, S., Ozer, N. K, Ricciarelli, R. and Spycher S. 1998. Molecular basis of α-tocopherol control of smooth muscle cell proliferation. Biofactors 7: 314. Berliner, J. A. and Heinecke, J. W. 1996. The role of oxidized lipoproteins in atherogenesis. Free Radical Biology and Medicine 20 (5): 707-727. Berliner, J. A., Navab, M., Fogelman, A. M., Frank, J. S., Demer, L. L., Edwards, P. A., Watson, A. D. and Lusis, A. J. 1995. Atherosclerosis: basic mechanisms. Oxidation, inflammation, and genetics. Circulation 91: 2488-2496. Brand, K., Page, S., Rogler, G., Bartsch, A., Brandl, R., Knuechel, R., Page, M., Kaltschmidt, C., Baeuerle, P. A. and Neumeier D. 1996. Activated transcription factor NF-kappa B is present in the atherosclerotic lesion. Journal of Clinical Investigations 97: 1715- 1722. Collins, T. 1993. Endothelial NF-kappaB and the initiation of the atherosclerotic lesions. Laboratory Investigations 68 : 499-506. Das, S., Powell, S. R., Wang, P., Divald, K., Nasaretnam, K. and Tosak, A. 2005. Cardioprotection with palm oil: antioxidant activity of tocotrienol linked with its ability to stabilize proteosomes. American Journal of Physiology. Heart and Circulatory Physiology 289: 361-367. Gee, P. T. 2011. Unleashing the untold and misunderstood observations of vitamin E. Genes Nutrition 6: 5-16. Gosling, J., Slaymaker, S., Gu, L., Tseng, S., Zlot, C. H., Young, S. G., Rollins, B. J. and Charo I. F. 1999. MCP-1 deficiency reduces susceptibility to atherosclerosis in mice that overexpress human apolipoprotein B. Journal of Clinical Investigations 103: 773-778. Gulcin, I., Mshvildadze, V., Gepdiremen, A. and Elias, R. 2006. Screening of antiradical and antioxidant activity of monodesmosides and crude extract from Leontice smirnowii tuber. Phytomedicine 13: 343-351. Kamat, J. P. and Devasagayamm, T. P. A., 1995. Tocotrienols from palm oil as potent inhibitors of lipid peroxidation and protein oxidation in rat brain mitochondria. Neuroscience letters 195: 179-182. Keaney, J. F. Jr., Gaziano, G. M., Xu, A., Frei, B., Curran Celantano, J., Shwaery, G. T., Loscalzo, J. and Vita, J. A. 1994. Low dose α-tocopherol improves and high dose α-tocopherol worsens endothelial vasodilators function in cholesterol fed rabbit. Clinical Investigations 93: 884-851. Kim, J. S. 2005. Radical scavenging capacity and antioxidant activity of the E vitamer fraction in rice bran. Journal of Food Science 70 (Nr. 3): 208-213. Lee, J. Y., Hwang, W. I. and Lim, S. T. 2004. Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. Journal of Ethnopharmacology 93: 409-415. Li, D. Y., Chen, H. J. and Mehta, J. L. 2001. Statins inhibit oxidized-LDL-mediated LOX-1 expression, uptake of oxidized-LDL and reduction in PKB phosphorylation. Cardiovascular Research 52: 130-135. Martin, A., Foxall, T., Blumberg, J. B. and Meydani, M. 1997. Vitamin E inhibits low density lipoprotein induced adhesion of monocytes to human aortic endothelial cells in vitro. Arteriosclerosis, Thrombosis and Vascular Biology 17:429-436. Miller, E. R., Barriuso, R. P., Dalal, D., Riemersma, R. A., Appel, L. J. and Guallar, E. 2005. Meta-analysis: High dosage vitamin E supplementation may increase all-cause mortality. Annals of Internal Medicine 142: 37-46. Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65(1-2): 55-63. Osawa, T. and Namiki, M. 1981. A novel type of antioxidant isolated from leaf wax of Eucalyptus leaves. Agricultural & Biological Chemistry 45(3): 735-739. Palozza, P., Verdecchia, S., Avanzi, L., Vertuani, S., Serini, S., Lannone, A. and Manfredini, S. 2006. Comparative antioxidant activity of tocotrienols and the novel chromanyl-polyisoprenyl molecule FeAox-6 in isolated membranes and intact cells. Molecular and Cellular Biochemistry 287: 21-32. Poolman, T. M., Nga, L. L., Farmer, P. B. and Manson, M. M. 2005. Inhibition of the respiratory burst by resveratrol in human monocytes: Correlation with inhibition of PI3K signaling. Free Radical Biology and Medicine 39: 118 -132. Qureshi, A. A., Bradlow, B. A., Brace, L., Mangello, J., Peterson, D. M., Pearce, B. C., Wright. J. J., Gapor, A. and Elson, C. E. 1995. Response of hypercholesterolaemic subjects to administration of tocotrienols. Lipids 30(12): 1171-1177. Ross, R. 1999. Atherosclerosis: an inflammatory disease. New England Journal of Medicine 340: 115-126. Sen, K. S., Khanna, S. and Roy, S. 2006. Tocotrienols: Vitamin E beyond tocopherols. Life Sciences 78: 2088-2098. Serbinova, E., Kagan, V., Han, D. and Packer, L. 1991. Free radical recycling and intramembrane mobility in the antioxidant properties of α-Tocopherol and α-Tocotrienol. Free Radical Biology and Medicine 10: 263-75. Soelaiman, I. N., Ahmad, N. S. and Khalid, B. A. K. 2004. Palm oil tocotrienol mixture better than α-tocopherol acetate in protecting bones against free – radical induced elevation of bone – resorbing cytokines. Asia Pacific Journal of Clinical Nutrition 13 (Suppl): S111. Tan, B. 2010. Tocotrienols: The New Vitamin E. Downloaded from http: www. Spacedoc.net on 11/01/2012. Theriault, A., Chao, T. C. and Gapor, A. 2002. Atherosclerosis 160: 21-30. Tribble, D. L. 1999. AHA Science Advisory. Antioxidant consumption and risk of coronary heart disease: emphasis on vitamin C, vitamin E and beta carotene: a statement from for healthcare professionals from the American Heart Association. Circulation 99: 591- 595. Vivekanathan, D. P., Penn, M. S., Sapp, S. H., Hsu, A. and Topol, E. J. 2003. Use of antioxidant vitamins for the prevention of cardiovascular disease:meta-analysis of randomised trials. Lancet 361: 2017-2023. Wang, H. and Joseph, J. A. 1999. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radical Biology and Medicine 27: 612-616.
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| spellingShingle |
Optimal antioxidant activity with moderate concentrations of Tocotrienol rich fraction (TRF) in in vitro assays
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| summary |
Vitamin E is known to have potent antioxidant activity and plays an important role in reducing oxidative stress, a pivotal step in atherogenesis. However, several randomised clinical trials using α-tocopherol have failed to demonstrate consistent beneficial effects of antioxidants against atherosclerosis and clinical endpoints. Tocotrienol, a vitamin E compound analogue is shown to have more potent antioxidant activity compared to tocopherol. Finding the optimal anti-oxidative dose is crucial and may effectively be applied for cardioprotection in human. The objective of this study was to determine the optimal dose of tocotrienol rich fraction (TRF) with highest antioxidant activity in vitro using the ferric thiocyanate (FTC), 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity and 2’, 7’- dichlorofluorescein diacetate (DCFHDA) assays. It was found that TRF exhibited potent antioxidant and free radical scavenging activities with an IC50 of 22.10 + 0.01 µg/ml. In all assays, TRF had optimal antioxidant activity at moderate concentrations (10-100 µg/ml). In conclusion, TRF has potent antioxidant activity, which is optimal at moderate concentrations.
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| title |
Optimal antioxidant activity with moderate concentrations of Tocotrienol rich fraction (TRF) in in vitro assays
|
| title_full |
Optimal antioxidant activity with moderate concentrations of Tocotrienol rich fraction (TRF) in in vitro assays
|
| title_fullStr |
Optimal antioxidant activity with moderate concentrations of Tocotrienol rich fraction (TRF) in in vitro assays
|
| title_full_unstemmed |
Optimal antioxidant activity with moderate concentrations of Tocotrienol rich fraction (TRF) in in vitro assays
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| title_short |
Optimal antioxidant activity with moderate concentrations of Tocotrienol rich fraction (TRF) in in vitro assays
|
| title_sort |
optimal antioxidant activity with moderate concentrations of tocotrienol rich fraction (trf) in in vitro assays
|