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1860799835108540416
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INTELEK Repository
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Online Access
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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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2011-10-24 10:08:07
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Restricted Document
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7582
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UniSZA
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1. Nowak T, Januszkiewicz D, Zawada M, Pernak M, Lewandowski K, Rembowska J, et al. Amplification of hTERT and hTERC genes in leukemic cells with high expression and activity of telomerase. Oncol Rep. 2006 Aug; 16(2):301-5. 2. Zhang A, Zheng C, Hou M, Lindvall C, Wallin KL, Angstrom T, et al. Amplification of the telomerase reverse transcriptase (hTERT) gene in cervical carcinomas. Genes Chromosomes Cancer. 2002 Jul; 34(3):269-75. 3. Ohyashiki K, Ohyashiki JH, Iwama H, Hayashi S, Shay JW, Toyama K. Telomerase activity and cytogenetic changes in chronic myeloid leukemia with disease progression. Leukemia. 1997 Feb; 11(2):190-4. 4. Mera SL. The role of telomeres in ageing and cancer. Br J Biomed Sci. 1998 Sep; 55(3):221-5. 5. Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PL, et al. Specific association of human telomerase activity with immortal cells and cancer. Science. 1994 Dec 23; 266(5193):2011-5. 6. Zhang A, Zheng C, Lindvall C, Hou M, Ekedahl J, Lewensohn R, et al. Frequent amplification of the telomerase reverse transcriptase gene in human tumors. Cancer Res. 2000 Nov 15; 60(22):6230-5. 7. Biedler JL, Helson L, Spengler BA. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res. 1973 Nov; 33(11):2643-52. 8. Van Stedum S, King W. Basic FISH techniques and troubleshooting. Methods Mol Biol. 2002; 204:51-63. 9. Bryce LA, Morrison N, Hoare SF, Muir S, Keith WN. Mapping of the gene for the human telomerase reverse transcriptase, hTERT, to chromosome 5p15.33 by fluorescence in situ hybridization. Neoplasia. 2000 May�Jun; 2(3):197-201. 10. Klein E, Ben-Bassat H, Neumann H, Ralph P, Zeuthen J, Polliack A, et al. Properties of the K562 cell line, derived from a patient with chronic myeloid leukemia. Int J Cancer. 1976 Oct 15; 18(4):421-31. 11. Cao Y, Bryan TM, Reddel RR. Increased copy number of the TERT and TERC telomerase subunit genes in cancer cells. Cancer Sci. 2008 Jun; 99(6):1092-9. 12. Zhu CQ, Cutz JC, Liu N, Lau D, Shepherd FA, Squire JA, et al. Amplification of telomerase (hTERT) gene is a poor prognostic marker in non-small-cell lung cancer. Br J Cancer. 2006 May 22; 94(10):1452-9. 13. Bieche I, Olivi M, Champeme MH, Vidaud D, Lidereau R, Vidaud M. Novel approach to quantitative polymerase chain reaction using real-time detection: application to the detection of gene amplification in breast cancer. Int J Cancer. 1998 Nov 23; 78(5):661-6. 14. Murnane JP, Sabatier L. Chromosome rearrangements resulting from telomere dysfunction and their role in cancer. Bioessays. 2004 Nov; 26(11):1164-74.
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3149-01-FH02-FSK-19-25054.pdf
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7582
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7582 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7582 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 5 1.6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in Adobe InDesign CS4 (6.0.5) 2011-10-24 10:08:07 3149-01-FH02-FSK-19-25054.pdf UniSZA Private Access Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis Malaysian Journal of Biochemistry and Molecular Biology Telomerase is ribonucleoprotein enzyme complex consisting of two main core components; reverse transcriptase (TERT), and RNA template for telomeric DNA synthesis (TERC). TERT gene acts as catalytic component in this enzyme and is located on chromosome 5p15. It has been reported that there is an amplification or increase of TERT gene copy number in human cancer cell lines and primary tumour samples which may associate with increased telomerase activity. In our study, dual-color assay probe was used in fluorescence in situ hybridization (FISH) analysis that covered the genomic region encoding TERT (Red signal) together with a specific sequence of EGR1 (Green signal) at 5q31 to facilitate chromosome identification. In normal cells, the FISH signal pattern was observed as two green signals and two red signals (2G2R). Amplification of the TERT gene has been detected in more than 90% of the cells in cell line in the leukaemia. Besides that, aneusomy of chromosome 5 was also detected indicating the presence of three copies of chromosome 5 with 3G3R signal pattern. Thus, the application of interphase FISH technique for detection of TERT gene amplification has been successfully optimized. 19 1 12-15 1. Nowak T, Januszkiewicz D, Zawada M, Pernak M, Lewandowski K, Rembowska J, et al. Amplification of hTERT and hTERC genes in leukemic cells with high expression and activity of telomerase. Oncol Rep. 2006 Aug; 16(2):301-5. 2. Zhang A, Zheng C, Hou M, Lindvall C, Wallin KL, Angstrom T, et al. Amplification of the telomerase reverse transcriptase (hTERT) gene in cervical carcinomas. Genes Chromosomes Cancer. 2002 Jul; 34(3):269-75. 3. Ohyashiki K, Ohyashiki JH, Iwama H, Hayashi S, Shay JW, Toyama K. Telomerase activity and cytogenetic changes in chronic myeloid leukemia with disease progression. Leukemia. 1997 Feb; 11(2):190-4. 4. Mera SL. The role of telomeres in ageing and cancer. Br J Biomed Sci. 1998 Sep; 55(3):221-5. 5. Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PL, et al. Specific association of human telomerase activity with immortal cells and cancer. Science. 1994 Dec 23; 266(5193):2011-5. 6. Zhang A, Zheng C, Lindvall C, Hou M, Ekedahl J, Lewensohn R, et al. Frequent amplification of the telomerase reverse transcriptase gene in human tumors. Cancer Res. 2000 Nov 15; 60(22):6230-5. 7. Biedler JL, Helson L, Spengler BA. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res. 1973 Nov; 33(11):2643-52. 8. Van Stedum S, King W. Basic FISH techniques and troubleshooting. Methods Mol Biol. 2002; 204:51-63. 9. Bryce LA, Morrison N, Hoare SF, Muir S, Keith WN. Mapping of the gene for the human telomerase reverse transcriptase, hTERT, to chromosome 5p15.33 by fluorescence in situ hybridization. Neoplasia. 2000 May�Jun; 2(3):197-201. 10. Klein E, Ben-Bassat H, Neumann H, Ralph P, Zeuthen J, Polliack A, et al. Properties of the K562 cell line, derived from a patient with chronic myeloid leukemia. Int J Cancer. 1976 Oct 15; 18(4):421-31. 11. Cao Y, Bryan TM, Reddel RR. Increased copy number of the TERT and TERC telomerase subunit genes in cancer cells. Cancer Sci. 2008 Jun; 99(6):1092-9. 12. Zhu CQ, Cutz JC, Liu N, Lau D, Shepherd FA, Squire JA, et al. Amplification of telomerase (hTERT) gene is a poor prognostic marker in non-small-cell lung cancer. Br J Cancer. 2006 May 22; 94(10):1452-9. 13. Bieche I, Olivi M, Champeme MH, Vidaud D, Lidereau R, Vidaud M. Novel approach to quantitative polymerase chain reaction using real-time detection: application to the detection of gene amplification in breast cancer. Int J Cancer. 1998 Nov 23; 78(5):661-6. 14. Murnane JP, Sabatier L. Chromosome rearrangements resulting from telomere dysfunction and their role in cancer. Bioessays. 2004 Nov; 26(11):1164-74.
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| spellingShingle |
Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
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| summary |
Telomerase is ribonucleoprotein enzyme complex consisting of two main core components; reverse transcriptase (TERT), and RNA template for telomeric DNA synthesis (TERC). TERT gene acts as catalytic component in this enzyme and is located on chromosome 5p15. It has been reported that there is an amplification or increase of TERT gene copy number in human cancer cell lines and primary tumour samples which may associate with increased telomerase activity. In our study, dual-color assay probe was used in fluorescence in situ hybridization (FISH) analysis that covered the genomic region encoding TERT (Red signal) together with a specific sequence of EGR1 (Green signal) at 5q31 to facilitate chromosome identification. In normal cells, the FISH signal pattern was observed as two green signals and two red signals (2G2R). Amplification of the TERT gene has been detected in more than 90% of the cells in cell line in the leukaemia. Besides that, aneusomy of chromosome 5 was also detected indicating the presence of three copies of chromosome 5 with 3G3R signal pattern. Thus, the application of interphase FISH technique for detection of TERT gene amplification has been successfully optimized.
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| title |
Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
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| title_full |
Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
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| title_fullStr |
Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
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| title_full_unstemmed |
Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
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| title_short |
Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
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| title_sort |
copy number status of tert gene in a myeloid leukaemia cell line, k562: application of interphase fluorescence in situ hybridization (fish) analysis
|