Hexavalent molybdenum reduction to Mo-blue by Acinetobacter calcoaceticus
| Format: | Restricted Document |
|---|
| _version_ | 1860799803698446336 |
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| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 |
| date | 2014-08-27 11:44:31 |
| format | Restricted Document |
| id | 7459 |
| institution | UniSZA |
| originalfilename | 2920-01-FH02-FPBSM-14-01227.jpg |
| person | UniSZA Unisza unisza |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7459 |
| spelling | 7459 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7459 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal UniSZA Unisza unisza image/jpeg inches 96 96 1403 94 94 2014-08-27 11:44:31 785 1403x785 2920-01-FH02-FPBSM-14-01227.jpg UniSZA Private Access Hexavalent molybdenum reduction to Mo-blue by Acinetobacter calcoaceticus Folia Microbiologica A local molybdenum-reducing bacterium was isolated and tentatively identified as Acinetobacter calcoaceticus strain Dr.Y12 based on carbon utilization profiles using Biolog GN plates and 16S rDNA comparative analysis. Molybdate reduction was optimized under conditions of low dissolved oxygen (37 degrees C and pH 6.5). Of the electron donors tested, glucose, fructose, maltose and sucrose supported molybdate reduction after 1 d of incubation, glucose and fructose supporting the highest Mo-blue production. Optimum Mo-blue production was reached at 20 mmol/L molybdate and 5 mmol/L phosphate; increasing the phosphate concentrations inhibited the production. An increase in an overall absorption profiles, especially at peak maximum at 865 nm and the shoulder at 700 nm, was observed in direct correlation with the increased in Mo-blue amounts. Metal ions, such as chromium, cadmium, copper, mercury and lead (2 mmol/L final concentration) caused approximately 88, 53, 80, 100, and 20 % inhibition, respectively. Respiratory inhibitors, such as antimycin A, rotenone, sodium azide and cyanide showed in this bacterium no inhibition of the Mo-blue production, suggesting that the electron transport system is not a site of molybdate reduction. 137-143 |
| spellingShingle | Hexavalent molybdenum reduction to Mo-blue by Acinetobacter calcoaceticus |
| summary | A local molybdenum-reducing bacterium was isolated and tentatively identified as Acinetobacter calcoaceticus strain Dr.Y12 based on carbon utilization profiles using Biolog GN plates and 16S rDNA comparative analysis. Molybdate reduction was optimized under conditions of low dissolved oxygen (37 degrees C and pH 6.5). Of the electron donors tested, glucose, fructose, maltose and sucrose supported molybdate reduction after 1 d of incubation, glucose and fructose supporting the highest Mo-blue production. Optimum Mo-blue production was reached at 20 mmol/L molybdate and 5 mmol/L phosphate; increasing the phosphate concentrations inhibited the production. An increase in an overall absorption profiles, especially at peak maximum at 865 nm and the shoulder at 700 nm, was observed in direct correlation with the increased in Mo-blue amounts. Metal ions, such as chromium, cadmium, copper, mercury and lead (2 mmol/L final concentration) caused approximately 88, 53, 80, 100, and 20 % inhibition, respectively. Respiratory inhibitors, such as antimycin A, rotenone, sodium azide and cyanide showed in this bacterium no inhibition of the Mo-blue production, suggesting that the electron transport system is not a site of molybdate reduction. |
| title | Hexavalent molybdenum reduction to Mo-blue by Acinetobacter calcoaceticus |
| title_full | Hexavalent molybdenum reduction to Mo-blue by Acinetobacter calcoaceticus |
| title_fullStr | Hexavalent molybdenum reduction to Mo-blue by Acinetobacter calcoaceticus |
| title_full_unstemmed | Hexavalent molybdenum reduction to Mo-blue by Acinetobacter calcoaceticus |
| title_short | Hexavalent molybdenum reduction to Mo-blue by Acinetobacter calcoaceticus |
| title_sort | hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus |