Antiulcer activity of Muntingia calabura leaves involves the modulation of endogenous nitric oxide and nonprotein sulfhydryl compounds

Bibliographic Details
Format: Restricted Document
_version_ 1860799803499216896
building INTELEK Repository
collection Online Access
collectionurl https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
date 2024-09-18 16:22:18
format Restricted Document
id 7458
institution UniSZA
originalfilename 2919-01-FH02-FBIM-15-02957.pdf
recordtype oai_dc
resourceurl https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7458
spelling 7458 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7458 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 2 1.6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in 2024-09-18 16:22:18 2919-01-FH02-FBIM-15-02957.pdf UniSZA Private Access Antiulcer activity of Muntingia calabura leaves involves the modulation of endogenous nitric oxide and nonprotein sulfhydryl compounds Pharm Biol. Abstract Context: Muntingia calabura L. (Muntingiaceae) is a native plant species of the American continent and is widely cultivated in warm areas in Asia, including Malaysia. The plant is traditionally used to relieve pain from gastric ulcers. Objective: This study was designed to determine the antiulcer activity of a methanol extract of M. calabura leaves (MEMC) and the possible mechanisms of action involved. Materials and methods: An acute toxicity study was conducted using a single oral dose of 2000 mg/kg MEMC. The antiulcer activity of MEMC was evaluated in absolute ethanol­ and indomethacin­induced gastric ulcer rat models. MEMC was administered orally (dose range 25­500 mg/kg) to rats fasted for 24 h. The animals were pretreated with N ­nitro­l­arginine methyl esters (l­NAME) or N­ethylmaleimide (NEM) prior to MEMC treatment to assess the possible involvement of endogenous nitric oxide (NO) and nonprotein sulfhydryl (NP­SH) compounds in the gastroprotective effect of MEMC. Results: As the administered dose did not cause toxicity in the rats, the oral median lethal dose (LD ) of MEMC was >2000 mg/kg in rats. MEMC exerted significant (p < 0.001) gastroprotective activity in the ethanol­ and indomethacin­induced ulcer models dose­dependently. Histological evaluation supported the observed antiulcer activity of MEMC. l­NAME and NEM pretreatment significantly (p < 0.05) reversed and abolished the gastroprotective effect of MEMC, respectively. Discussion and conclusion: The results obtained indicate that MEMC has significant antiulcer activity that might involve the participation of endogenous NO and NP­SH compounds. These findings provide new pharmacological information regarding the potential use of M. calabura 52 4 410-418
spellingShingle Antiulcer activity of Muntingia calabura leaves involves the modulation of endogenous nitric oxide and nonprotein sulfhydryl compounds
summary Abstract Context: Muntingia calabura L. (Muntingiaceae) is a native plant species of the American continent and is widely cultivated in warm areas in Asia, including Malaysia. The plant is traditionally used to relieve pain from gastric ulcers. Objective: This study was designed to determine the antiulcer activity of a methanol extract of M. calabura leaves (MEMC) and the possible mechanisms of action involved. Materials and methods: An acute toxicity study was conducted using a single oral dose of 2000 mg/kg MEMC. The antiulcer activity of MEMC was evaluated in absolute ethanol­ and indomethacin­induced gastric ulcer rat models. MEMC was administered orally (dose range 25­500 mg/kg) to rats fasted for 24 h. The animals were pretreated with N ­nitro­l­arginine methyl esters (l­NAME) or N­ethylmaleimide (NEM) prior to MEMC treatment to assess the possible involvement of endogenous nitric oxide (NO) and nonprotein sulfhydryl (NP­SH) compounds in the gastroprotective effect of MEMC. Results: As the administered dose did not cause toxicity in the rats, the oral median lethal dose (LD ) of MEMC was >2000 mg/kg in rats. MEMC exerted significant (p < 0.001) gastroprotective activity in the ethanol­ and indomethacin­induced ulcer models dose­dependently. Histological evaluation supported the observed antiulcer activity of MEMC. l­NAME and NEM pretreatment significantly (p < 0.05) reversed and abolished the gastroprotective effect of MEMC, respectively. Discussion and conclusion: The results obtained indicate that MEMC has significant antiulcer activity that might involve the participation of endogenous NO and NP­SH compounds. These findings provide new pharmacological information regarding the potential use of M. calabura
title Antiulcer activity of Muntingia calabura leaves involves the modulation of endogenous nitric oxide and nonprotein sulfhydryl compounds
title_full Antiulcer activity of Muntingia calabura leaves involves the modulation of endogenous nitric oxide and nonprotein sulfhydryl compounds
title_fullStr Antiulcer activity of Muntingia calabura leaves involves the modulation of endogenous nitric oxide and nonprotein sulfhydryl compounds
title_full_unstemmed Antiulcer activity of Muntingia calabura leaves involves the modulation of endogenous nitric oxide and nonprotein sulfhydryl compounds
title_short Antiulcer activity of Muntingia calabura leaves involves the modulation of endogenous nitric oxide and nonprotein sulfhydryl compounds
title_sort antiulcer activity of muntingia calabura leaves involves the modulation of endogenous nitric oxide and nonprotein sulfhydryl compounds