Effects of Carbon and Nitrogen Sources and Carbon-to-Nitrogen Ratio on Production of Exserohilum longirostratum

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building INTELEK Repository
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collectionurl https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
date 2013-07-09 10:44:26
format Restricted Document
id 7430
institution UniSZA
originalfilename 2875-01-FH02-FBIM-16-06625.pdf
person Matzakaria
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resourceurl https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7430
spelling 7430 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7430 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 11 1.6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in Matzakaria 2013-07-09 10:44:26 2875-01-FH02-FBIM-16-06625.pdf UniSZA Private Access Effects of Carbon and Nitrogen Sources and Carbon-to-Nitrogen Ratio on Production of Exserohilum longirostratum Journal of Agrobiotechnology The effects of carbon and nitrogen sources and the carbon-to-nitrogen ratio on the growth and sporulation of Exserohilum longirostratum were evaluated. Rice flour and malt extracts as the carbon and nitrogen sources, respectively, produced the greatest amount of mycelium. Sources that produced the most biomass were chosen as carbon and nitrogen sources in a C:N ratio test. However, in further experiments, glucose was chosen as the carbon source for the C:N ratio test because rice flour was found to be easily contaminated. Under the C:N ratio test (fixed carbon test), the highest spore production was obtained with a 5:1 ratio (4.78 × 106 spores/mL) and the highest biomass production was obtained with a 7.5:1 ratio (4.66 g/100 mL). In the fixed nitrogen test, 7.5:1 ratio provided the greatest output (4.08 × 106 spores/mL) whereas a 5:1 ratio produced the most biomass (4.33 g/100 mL). Meanwhile, the control which consisted of V8 agar without additional carbon and nitrogen source produced 1.07 × 106 spores/mL. These results provide information on the influence of carbon and nitrogen source and the C:N ratio that can be used in media for optimum growth and spore yield 4 1 57-67
spellingShingle Effects of Carbon and Nitrogen Sources and Carbon-to-Nitrogen Ratio on Production of Exserohilum longirostratum
summary The effects of carbon and nitrogen sources and the carbon-to-nitrogen ratio on the growth and sporulation of Exserohilum longirostratum were evaluated. Rice flour and malt extracts as the carbon and nitrogen sources, respectively, produced the greatest amount of mycelium. Sources that produced the most biomass were chosen as carbon and nitrogen sources in a C:N ratio test. However, in further experiments, glucose was chosen as the carbon source for the C:N ratio test because rice flour was found to be easily contaminated. Under the C:N ratio test (fixed carbon test), the highest spore production was obtained with a 5:1 ratio (4.78 × 106 spores/mL) and the highest biomass production was obtained with a 7.5:1 ratio (4.66 g/100 mL). In the fixed nitrogen test, 7.5:1 ratio provided the greatest output (4.08 × 106 spores/mL) whereas a 5:1 ratio produced the most biomass (4.33 g/100 mL). Meanwhile, the control which consisted of V8 agar without additional carbon and nitrogen source produced 1.07 × 106 spores/mL. These results provide information on the influence of carbon and nitrogen source and the C:N ratio that can be used in media for optimum growth and spore yield
title Effects of Carbon and Nitrogen Sources and Carbon-to-Nitrogen Ratio on Production of Exserohilum longirostratum
title_full Effects of Carbon and Nitrogen Sources and Carbon-to-Nitrogen Ratio on Production of Exserohilum longirostratum
title_fullStr Effects of Carbon and Nitrogen Sources and Carbon-to-Nitrogen Ratio on Production of Exserohilum longirostratum
title_full_unstemmed Effects of Carbon and Nitrogen Sources and Carbon-to-Nitrogen Ratio on Production of Exserohilum longirostratum
title_short Effects of Carbon and Nitrogen Sources and Carbon-to-Nitrogen Ratio on Production of Exserohilum longirostratum
title_sort effects of carbon and nitrogen sources and carbon-to-nitrogen ratio on production of exserohilum longirostratum