Eleven novel polymorphic microsatellite loci for oval squid Sepioteuthis lessoniana (Shiro-Ika Type)

Bibliographic Details
Format: Restricted Document
_version_ 1860799779758407680
building INTELEK Repository
collection Online Access
collectionurl https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
date 2016-10-12 11:09:01
format Restricted Document
id 7363
institution UniSZA
originalfilename 2776-01-FH02-FBIM-16-06733.jpg
person norman
recordtype oai_dc
resourceurl https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7363
spelling 7363 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7363 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal image/jpeg inches 96 96 norman 768 1426 68 68 2016-10-12 11:09:01 1426x768 2776-01-FH02-FBIM-16-06733.jpg UniSZA Private Access Eleven novel polymorphic microsatellite loci for oval squid Sepioteuthis lessoniana (Shiro-Ika Type) View at Publisher| Export | Download | Add to List | More... International Journal of Molecular Sciences The oval squid Sepioteuthis lessoniana is one of the most economically important squid species in Japan; however, its population structure is poorly understood due to the lack of hypervariable markers. Such information is critical for managing sustainable fisheries, as well as for ensuring the existence of wild S. lessoniana stocks. Eleven candidate microsatellite loci were isolated from a small insert genomic DNA library. Polymorphisms in these 11 loci were screened in 24 wild individuals. The number of alleles per locus was found to range from 5 to 19 alleles, and the observed heterozygosity ranged from 0.292 to 0.958. No evidence for linkage disequilibrium was detected among all the loci. The genotypic proportions conformed to Hardy-Weinberg equilibrium, except at one locus. In conclusion, these polymorphic microsatellite loci may be used to develop a genetic framework to manage S. lessoniana in the future. 2013 by the authors; licensee MDPI, Basel, Switzerland 14 10 19971-19975
spellingShingle Eleven novel polymorphic microsatellite loci for oval squid Sepioteuthis lessoniana (Shiro-Ika Type)
summary The oval squid Sepioteuthis lessoniana is one of the most economically important squid species in Japan; however, its population structure is poorly understood due to the lack of hypervariable markers. Such information is critical for managing sustainable fisheries, as well as for ensuring the existence of wild S. lessoniana stocks. Eleven candidate microsatellite loci were isolated from a small insert genomic DNA library. Polymorphisms in these 11 loci were screened in 24 wild individuals. The number of alleles per locus was found to range from 5 to 19 alleles, and the observed heterozygosity ranged from 0.292 to 0.958. No evidence for linkage disequilibrium was detected among all the loci. The genotypic proportions conformed to Hardy-Weinberg equilibrium, except at one locus. In conclusion, these polymorphic microsatellite loci may be used to develop a genetic framework to manage S. lessoniana in the future. 2013 by the authors; licensee MDPI, Basel, Switzerland
title Eleven novel polymorphic microsatellite loci for oval squid Sepioteuthis lessoniana (Shiro-Ika Type)
title_full Eleven novel polymorphic microsatellite loci for oval squid Sepioteuthis lessoniana (Shiro-Ika Type)
title_fullStr Eleven novel polymorphic microsatellite loci for oval squid Sepioteuthis lessoniana (Shiro-Ika Type)
title_full_unstemmed Eleven novel polymorphic microsatellite loci for oval squid Sepioteuthis lessoniana (Shiro-Ika Type)
title_short Eleven novel polymorphic microsatellite loci for oval squid Sepioteuthis lessoniana (Shiro-Ika Type)
title_sort eleven novel polymorphic microsatellite loci for oval squid sepioteuthis lessoniana (shiro-ika type)