Bacterial reduction of hexavalent molybdenum to molybdenum blue
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| date | 2014-08-27 11:48:53 |
| format | Restricted Document |
| id | 7334 |
| institution | UniSZA |
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| spelling | 7334 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7334 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal UniSZA Unisza unisza image/jpeg inches 96 96 1417 797 16 16 2014-08-27 11:48:53 1417x797 2746-01-FH02-FPBSM-14-01228.jpg UniSZA Private Access Bacterial reduction of hexavalent molybdenum to molybdenum blue World Journal of Microbiology and Biotechnology A bacterium that was able to tolerate and reduce as high as 50 mM of sodium molybdate to molybdenum blue has been isolated from a metal recycling ground. The isolate was tentatively identified as Serratia sp. strain Dr.Y8 based on the carbon utilization profiles using Biology GN plates and partial 16S rDNA molecular phylogeny. ANOVA analysis showed that isolate Dr.Y8 produced significantly higher (P < 0.05) amount of Mo-blue with 3, 5 .1 and 11.3 times more molybdenum blue than previously isolated molybdenum reducers such as Serratia marcescens strain Dr.Y6,E. coli K12 and E. cloacae strain 48,respectively.Its molybdate reduction characteristics were studied in this work. Electron donor sources such as sucrose, mannitol, fructose, glucose and starch supported molybdate reduction. The optimum phosphate, pH and temperature that supported molybdate reduction were 5mM, pH 6.0 and 37'C,respectively.The molybdenum blue produced from cellular reduction exhibited a unique absorption spectrum with a maximum peak at 865 nm and a shoulder at 700 nm. Metal ions such as chromium, silver, copper and mercury resulted in approximately 61, 57.80 , and 69% inhibition of the molybdenum-reducing activity at 1 mM, respectively. The reduction characteristics of strain Dr.Y8 suggest that it would be useful in future molybdenum bioremediation. © 2009 Springer Science+Business Media B.V. 25 7 1225-1234 |
| spellingShingle | Bacterial reduction of hexavalent molybdenum to molybdenum blue |
| summary | A bacterium that was able to tolerate and reduce as high as 50 mM of sodium molybdate to molybdenum blue has been isolated from a metal recycling ground. The isolate was tentatively identified as Serratia sp. strain Dr.Y8 based on the carbon utilization profiles using Biology GN plates and partial 16S rDNA molecular phylogeny. ANOVA analysis showed that isolate Dr.Y8 produced significantly higher (P < 0.05) amount of Mo-blue with 3, 5 .1 and 11.3 times more molybdenum blue than previously isolated molybdenum reducers such as Serratia marcescens strain Dr.Y6,E. coli K12 and E. cloacae strain 48,respectively.Its molybdate reduction characteristics were studied in this work. Electron donor sources such as sucrose, mannitol, fructose, glucose and starch supported molybdate reduction. The optimum phosphate, pH and temperature that supported molybdate reduction were 5mM, pH 6.0 and 37'C,respectively.The molybdenum blue produced from cellular reduction exhibited a unique absorption spectrum with a maximum peak at 865 nm and a shoulder at 700 nm. Metal ions such as chromium, silver, copper and mercury resulted in approximately 61, 57.80 , and 69% inhibition of the molybdenum-reducing activity at 1 mM, respectively. The reduction characteristics of strain Dr.Y8 suggest that it would be useful in future molybdenum bioremediation. © 2009 Springer Science+Business Media B.V. |
| title | Bacterial reduction of hexavalent molybdenum to molybdenum blue |
| title_full | Bacterial reduction of hexavalent molybdenum to molybdenum blue |
| title_fullStr | Bacterial reduction of hexavalent molybdenum to molybdenum blue |
| title_full_unstemmed | Bacterial reduction of hexavalent molybdenum to molybdenum blue |
| title_short | Bacterial reduction of hexavalent molybdenum to molybdenum blue |
| title_sort | bacterial reduction of hexavalent molybdenum to molybdenum blue |