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1860799759705440256
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| building |
INTELEK Repository
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| collection |
Online Access
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| collectionurl |
https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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| date |
2017-11-19 15:52:22
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| format |
Restricted Document
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| id |
7283
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| institution |
UniSZA
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2672-01-FH02-FBIM-17-10891.jpg
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| person |
norman
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| recordtype |
oai_dc
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| resourceurl |
https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7283
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| spelling |
7283 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7283 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal image/jpeg inches 96 96 norman 54 54 1437 722 2017-11-19 15:52:22 1437x722 2672-01-FH02-FBIM-17-10891.jpg UniSZA Private Access In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates Walailak Journal of Science and Technology The aims of this study were to validate the primers developed for molecular-based detection and identification of Malaysian clinical isolates of methicilin-resistance Staphylococcus au reus (MRSA) using in-silico Polymerase Chain Reaction (PCR) and real-time PCR SYBR with Green I. Rapid molecular diagnostic and risk assessment of the MRSA are possible by real-time PCR SYBR Green I. However, validation of such primers for real-life samples is expensive and time consuming. Hence, development and verification of real-time PCR primers by in-silico PCR can be the first step I' • I • Igo .os es - • II- • • • 'I . • ' • 8, 10 3 237-246 https://www.scopus.com/record/display.uri?eid=2-s2.0-8488424
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| spellingShingle |
In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates
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| summary |
The aims of this study were to validate the primers developed for molecular-based detection and identification of Malaysian clinical isolates of methicilin-resistance Staphylococcus au reus (MRSA) using in-silico Polymerase Chain Reaction (PCR) and real-time PCR SYBR with Green I. Rapid molecular diagnostic and risk assessment of the MRSA are possible by real-time PCR SYBR Green I. However, validation of such primers for real-life samples is expensive and time consuming. Hence, development and verification of real-time PCR primers by in-silico PCR can be the first step I' • I • Igo .os es - • II- • • • 'I . • ' • 8,
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| title |
In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates
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| title_full |
In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates
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| title_fullStr |
In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates
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| title_full_unstemmed |
In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates
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| title_short |
In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates
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| title_sort |
in silico pcr verification and simplex real-time pcr detection of methicillin-resistant staphylococcus aureus (mrsa) from east coast malaysian clinical isolates
|