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1860799757728874496
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| building |
INTELEK Repository
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Online Access
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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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| date |
2013-08-01 15:23:03
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| format |
Restricted Document
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| id |
7276
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| institution |
UniSZA
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2660-01-FH02-FBIM-21-54197.pdf
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administrator
Administrator
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oai_dc
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7276
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| spelling |
7276 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7276 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 10 1.6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in administrator Administrator 2013-08-01 15:23:03 2660-01-FH02-FBIM-21-54197.pdf UniSZA Private Access Proteome analysis in the discovery of serological pregnancy biomarkers in damascus goats Journal of Agricultural Science and Technology A Early detection of pregnancy is advantageous to breeders. However, tools that were used in the early pregnancy detection are expensive, laboratory-based and not efficient and applicable enough in the field to farmers. Thus, there is a need to find a biomarker which not only can detect pregnancy but also could be applied to the livestock on the field. Proteomic approach was used in this study as to search for early pregnancy biomarker. When goat sera at day 14 of gestation were resolved by using two dimensional gel electrophoresis, five differentially expressed proteins were detected. Four of the proteins were identified as albumin precursors, immunoglobulin lambda light chain and zinc-alpha-2-glycoprotein. Pregnancy associated glycoprotein-1 was detected by using liquid chromatography mass spectrometry and the protein was validated by immunoblotting. These proteins have potential to be used as a biomarker for early pregnancy detection, however, more extensive analysis are needed to validate its possibility. 3 465-473
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| spellingShingle |
Proteome analysis in the discovery of serological pregnancy biomarkers in damascus goats
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| summary |
Early detection of pregnancy is advantageous to breeders. However, tools that were used in the early pregnancy detection are expensive, laboratory-based and not efficient and applicable enough in the field to farmers. Thus, there is a need to find a biomarker which not only can detect pregnancy but also could be applied to the livestock on the field. Proteomic approach was used in this study as to search for early pregnancy biomarker. When goat sera at day 14 of gestation were resolved by using two dimensional gel electrophoresis, five differentially expressed proteins were detected. Four of the proteins were identified as albumin precursors, immunoglobulin lambda light chain and zinc-alpha-2-glycoprotein. Pregnancy associated glycoprotein-1 was detected by using liquid chromatography mass spectrometry and the protein was validated by immunoblotting. These proteins have potential to be used as a biomarker for early pregnancy detection, however, more extensive analysis are needed to validate its possibility.
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| title |
Proteome analysis in the discovery of serological pregnancy biomarkers in damascus goats
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| title_full |
Proteome analysis in the discovery of serological pregnancy biomarkers in damascus goats
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| title_fullStr |
Proteome analysis in the discovery of serological pregnancy biomarkers in damascus goats
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| title_full_unstemmed |
Proteome analysis in the discovery of serological pregnancy biomarkers in damascus goats
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| title_short |
Proteome analysis in the discovery of serological pregnancy biomarkers in damascus goats
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| title_sort |
proteome analysis in the discovery of serological pregnancy biomarkers in damascus goats
|