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1860799727385182208
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INTELEK Repository
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Online Access
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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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2012-04-07 16:39:31
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Restricted Document
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7158
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UniSZA
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2365-01-FH02-FPBSM-14-01240.pdf
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HP
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oai_dc
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7158
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7158 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=7158 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 5 1.6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in HP 2012-04-07 16:39:31 2365-01-FH02-FPBSM-14-01240.pdf UniSZA Private Access Induction of apoptosis by newcastle disease virus strains AF220 and V4-UPM in human promyelocytic leukemia (HL60) and human T- lymphoblastic leukemia (CEM -SS) Cells World academy of science, engineering and technology Newcastle Disease Virus (NDV), an avian paramyxovirus, is a highly contagious, generalised virus disease of domestic poultry and wild birds characterized by gastro-intestinal, respiratory and nervous signs. In this study, it was shown that NDV strain AF2240 and V4-UPM are cytolytic to Human Promyelocytic Leukemia, HL60 and Human T-lymphoblastic Leukemia, CEM-SS cells. Results from MTT cytolytic assay showed that CD50 for NDV AF2240 against HL60 was 130 HAU and NDV V4-UPM against HL60 and CEM-SS were 110.6 and 150.9 HAU respectively. Besides, both strains were found to inhibit the proliferation of cells in a dose dependent manner. The mode of cell death either by apoptosis or necrosis was further analyzed using acridine orange and propidium iodide (AO/PI) staining. Our results showed that both NDV strains induced primarily apoptosis in treated cells at CD50 concentration. In conclusion, both NDV strains caused cytolytic effects primarily via apoptosis in leukemia cells. 356-360
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Induction of apoptosis by newcastle disease virus strains AF220 and V4-UPM in human promyelocytic leukemia (HL60) and human T- lymphoblastic leukemia (CEM -SS) Cells
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| summary |
Newcastle Disease Virus (NDV), an avian paramyxovirus, is a highly contagious, generalised virus disease of domestic poultry and wild birds characterized by gastro-intestinal, respiratory and nervous signs. In this study, it was shown that NDV strain AF2240 and V4-UPM are cytolytic to Human Promyelocytic Leukemia, HL60 and Human T-lymphoblastic Leukemia, CEM-SS cells. Results from MTT cytolytic assay showed that CD50 for NDV AF2240 against HL60 was 130 HAU and NDV V4-UPM against HL60 and CEM-SS were 110.6 and 150.9 HAU respectively. Besides, both strains were found to inhibit the proliferation of cells in a dose dependent manner. The mode of cell death either by apoptosis or necrosis was further analyzed using acridine orange and propidium iodide (AO/PI) staining. Our results showed that both NDV strains induced primarily apoptosis in treated cells at CD50 concentration. In conclusion, both NDV strains caused cytolytic effects primarily via apoptosis in leukemia cells.
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| title |
Induction of apoptosis by newcastle disease virus strains AF220 and V4-UPM in human promyelocytic leukemia (HL60) and human T- lymphoblastic leukemia (CEM -SS) Cells
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| title_full |
Induction of apoptosis by newcastle disease virus strains AF220 and V4-UPM in human promyelocytic leukemia (HL60) and human T- lymphoblastic leukemia (CEM -SS) Cells
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| title_fullStr |
Induction of apoptosis by newcastle disease virus strains AF220 and V4-UPM in human promyelocytic leukemia (HL60) and human T- lymphoblastic leukemia (CEM -SS) Cells
|
| title_full_unstemmed |
Induction of apoptosis by newcastle disease virus strains AF220 and V4-UPM in human promyelocytic leukemia (HL60) and human T- lymphoblastic leukemia (CEM -SS) Cells
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| title_short |
Induction of apoptosis by newcastle disease virus strains AF220 and V4-UPM in human promyelocytic leukemia (HL60) and human T- lymphoblastic leukemia (CEM -SS) Cells
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| title_sort |
induction of apoptosis by newcastle disease virus strains af220 and v4-upm in human promyelocytic leukemia (hl60) and human t- lymphoblastic leukemia (cem -ss) cells
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