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1860799684727013376
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INTELEK Repository
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Online Access
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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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| date |
2011-10-24 10:08:07
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| format |
Restricted Document
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| id |
6993
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UniSZA
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2053-01-FH02-FSK-19-25054.pdf
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Adobe InDesign CS4 (6.0.5)
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oai_dc
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=6993
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6993 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=6993 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Article Journal application/pdf 5 1.6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in Adobe InDesign CS4 (6.0.5) 2011-10-24 10:08:07 2053-01-FH02-FSK-19-25054.pdf UniSZA Private Access Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis Malaysian journal of biochemistry and molecular biology Telomeraseisribonucleoproteinenzymecomplexconsistingoftwomaincorecomponents;reversetranscriptase(TERT),andRNAtemplatefortelomericDNAsynthesis(TERC).TERTgeneactsascatalyticcomponentinthisenzymeandislocatedonchromosome5p15.IthasbeenreportedthatthereisanamplificationorincreaseofTERTgenecopynumberinhumancancercelllinesandprimarytumoursampleswhichmayassociatewithincreasedtelomeraseactivity.Inourstudy,dual-colorassayprobewasusedinfluorescencein situhybridization(FISH)analysisthatcoveredthegenomicregionencodingTERT(Redsignal)togetherwithaspecificsequenceofEGR1(Greensignal)at5q31tofacilitatechromosomeidentification.Innormalcells,theFISHsignalpatternwasobservedastwogreensignalsandtworedsignals(2G2R).AmplificationoftheTERTgenehasbeendetectedinmorethan90%ofthecellsincelllineintheleukaemia.Besidesthat,aneusomyofchromosome5wasalsodetectedindicatingthepresenceofthreecopiesofchromosome5with3G3Rsignalpattern.Thus,theapplicationofinterphaseFISHtechniquefordetectionofTERTgeneamplificationhasbeensuccessfullyoptimized 19 1 12-15
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| spellingShingle |
Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
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| summary |
Telomeraseisribonucleoproteinenzymecomplexconsistingoftwomaincorecomponents;reversetranscriptase(TERT),andRNAtemplatefortelomericDNAsynthesis(TERC).TERTgeneactsascatalyticcomponentinthisenzymeandislocatedonchromosome5p15.IthasbeenreportedthatthereisanamplificationorincreaseofTERTgenecopynumberinhumancancercelllinesandprimarytumoursampleswhichmayassociatewithincreasedtelomeraseactivity.Inourstudy,dual-colorassayprobewasusedinfluorescencein situhybridization(FISH)analysisthatcoveredthegenomicregionencodingTERT(Redsignal)togetherwithaspecificsequenceofEGR1(Greensignal)at5q31tofacilitatechromosomeidentification.Innormalcells,theFISHsignalpatternwasobservedastwogreensignalsandtworedsignals(2G2R).AmplificationoftheTERTgenehasbeendetectedinmorethan90%ofthecellsincelllineintheleukaemia.Besidesthat,aneusomyofchromosome5wasalsodetectedindicatingthepresenceofthreecopiesofchromosome5with3G3Rsignalpattern.Thus,theapplicationofinterphaseFISHtechniquefordetectionofTERTgeneamplificationhasbeensuccessfullyoptimized
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| title |
Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
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| title_full |
Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
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| title_fullStr |
Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
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| title_full_unstemmed |
Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
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| title_short |
Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
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| title_sort |
copy number status of tert gene in a myeloid leukaemia cell line, k562: application of interphase fluorescence in situ hybridization (fish) analysis
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