Effect of Glycosaminoglycans Extract From Sea Cucumber Stichopus vastus on Inflammatory Mediators and Tissue Healing on Rodents With Full Thickness Burn Injury

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date 2017-08-22 09:54:09
eventvenue Sejong University, Seoul
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id 6518
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originalfilename 1449-01-FH03-FP-17-10517.pdf
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spelling 6518 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=6518 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Conference Conference Paper application/pdf 15 Adobe Acrobat Pro DC 20 Paper Capture Plug-in 1.7 Microsoft® Word 2016 2017-08-22 09:54:09 1449-01-FH03-FP-17-10517.pdf UniSZA Private Access Effect of Glycosaminoglycans Extract From Sea Cucumber Stichopus vastus on Inflammatory Mediators and Tissue Healing on Rodents With Full Thickness Burn Injury This study was design to investigate the effect of total sulfated GAG from integument body wall of sea cucumber Stichopus vastus on inflammatory mediators and tissue healing on rodents with full thickness burn injury. Twelve male Sprague-dawley rats weighting 350-400 grams were distributed randomly into two groups. Six rats in group A received 5 ml/kg/day intraperitoneal total sulfated GAG from S.vastus for 5 days, while control group received phosphate buffered saline (PBS). Level of IL-10 and IL-12 were measured at day 1, 6 and 12. The level of IL-10 revealed significantly (p<0.05) higher and the level of IL-12 was reduced significantly (p<0.05) in sulfated GAG treated group. Data suggested that sulfated GAG from S. vastus integument body wall increase antiinflammatory cytokine and reduce pro-inflammatory cytokine. Microscopic evaluation showed reduced neutrophils infiltration with enhanced proliferation of fibroblasts and angiogenesis (p<0.05) in the sulfated GAG treated group. Sulfated GAG from S. vastus integument body wall has impact on the outcome of wound healing process by increasing the level of anti-inflammatory cytokine, IL-10 and reduced level of pro-inflammatory cytokine, IL-12 and positive effect on fibroblast proliferation and angiogenesis process. 1st International Halal Management Conference (IHMC) 2017 Sejong University, Seoul
spellingShingle Effect of Glycosaminoglycans Extract From Sea Cucumber Stichopus vastus on Inflammatory Mediators and Tissue Healing on Rodents With Full Thickness Burn Injury
summary This study was design to investigate the effect of total sulfated GAG from integument body wall of sea cucumber Stichopus vastus on inflammatory mediators and tissue healing on rodents with full thickness burn injury. Twelve male Sprague-dawley rats weighting 350-400 grams were distributed randomly into two groups. Six rats in group A received 5 ml/kg/day intraperitoneal total sulfated GAG from S.vastus for 5 days, while control group received phosphate buffered saline (PBS). Level of IL-10 and IL-12 were measured at day 1, 6 and 12. The level of IL-10 revealed significantly (p<0.05) higher and the level of IL-12 was reduced significantly (p<0.05) in sulfated GAG treated group. Data suggested that sulfated GAG from S. vastus integument body wall increase antiinflammatory cytokine and reduce pro-inflammatory cytokine. Microscopic evaluation showed reduced neutrophils infiltration with enhanced proliferation of fibroblasts and angiogenesis (p<0.05) in the sulfated GAG treated group. Sulfated GAG from S. vastus integument body wall has impact on the outcome of wound healing process by increasing the level of anti-inflammatory cytokine, IL-10 and reduced level of pro-inflammatory cytokine, IL-12 and positive effect on fibroblast proliferation and angiogenesis process.
title Effect of Glycosaminoglycans Extract From Sea Cucumber Stichopus vastus on Inflammatory Mediators and Tissue Healing on Rodents With Full Thickness Burn Injury
title_full Effect of Glycosaminoglycans Extract From Sea Cucumber Stichopus vastus on Inflammatory Mediators and Tissue Healing on Rodents With Full Thickness Burn Injury
title_fullStr Effect of Glycosaminoglycans Extract From Sea Cucumber Stichopus vastus on Inflammatory Mediators and Tissue Healing on Rodents With Full Thickness Burn Injury
title_full_unstemmed Effect of Glycosaminoglycans Extract From Sea Cucumber Stichopus vastus on Inflammatory Mediators and Tissue Healing on Rodents With Full Thickness Burn Injury
title_short Effect of Glycosaminoglycans Extract From Sea Cucumber Stichopus vastus on Inflammatory Mediators and Tissue Healing on Rodents With Full Thickness Burn Injury
title_sort effect of glycosaminoglycans extract from sea cucumber stichopus vastus on inflammatory mediators and tissue healing on rodents with full thickness burn injury