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1860799549016113152
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INTELEK Repository
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Online Access
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https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
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2018-12-12 16:45:21
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UNISZA
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Restricted Document
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6433
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UniSZA
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1333-01-FH03-FBIM-19-23071.pdf
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oai_dc
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https://intelek.unisza.edu.my/intelek/pages/view.php?ref=6433
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6433 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=6433 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Conference Conference Paper application/pdf 4 Adobe Acrobat Pro DC 20 Paper Capture Plug-in 1.7 2018-12-12 16:45:21 1333-01-FH03-FBIM-19-23071.pdf UniSZA Private Access Detection and characterization of vibrio parahaemolyticus in raw mackerel fish and fish-based street food This study aimed to investigate the prevalence of Vibrio parahaemolyticus in raw mackerel fish as well as fish-based products namely satar and otak-otak. A total of 120 fish, 90 satar and 35 otak-otak that have been purchased randomly in Terengganu were subjected to PCR and conventional. plating method for detection and isolation of V parahaemo!J1ticus. The isolates were characterized by 12 types of antibiotic using discs diffusion method. About 67.5%, 23.3% and 5.71% of fish, satar and otak-otak were contaminated with V parahaemolyticus. A total of 58 V parahaemolyticus isolates were recovered from fish. Among them, 24 of V parahaemolyticus isolates carried tdh gene and 3 isolates had trh gene. All the isolates were susceptible to amikacin, norfloxacin, ceftazidime, ciprofloxacin, and enrofloxacin. There was high resistant towards penicillin (93.1%), vancomycin (89.7%) and ampicillin (55.2%). About 56.9% of strains isolated showed a MAR index higher than 0.2, indicating these strains were from high risk sources of contamination. This study found V parahae111o!Jticus contaminated raw fish mackerel, satar and otak-otak. International Conference on Agriculture, Animal Sciences and Food Technology (ICAFT) 2018 UNISZA
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| spellingShingle |
Detection and characterization of vibrio parahaemolyticus in raw mackerel fish and fish-based street food
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| summary |
This study aimed to investigate the prevalence of Vibrio parahaemolyticus in raw mackerel fish as well as fish-based products namely satar and otak-otak. A total of 120 fish, 90 satar and 35 otak-otak that have been purchased randomly in Terengganu were subjected to PCR and conventional. plating method for detection and isolation of V parahaemo!J1ticus. The isolates were characterized by 12 types of antibiotic using discs diffusion method. About 67.5%, 23.3% and 5.71% of fish, satar and otak-otak were contaminated with V parahaemolyticus. A total of 58 V parahaemolyticus isolates were recovered from fish. Among them, 24 of V parahaemolyticus isolates carried tdh gene and 3 isolates had trh gene. All the isolates were susceptible to amikacin, norfloxacin, ceftazidime, ciprofloxacin, and enrofloxacin. There was high resistant towards penicillin (93.1%), vancomycin (89.7%) and ampicillin (55.2%). About 56.9% of strains isolated showed a MAR index higher than 0.2, indicating these strains were from high risk sources of contamination. This study found V parahae111o!Jticus contaminated raw fish mackerel, satar and otak-otak.
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| title |
Detection and characterization of vibrio parahaemolyticus in raw mackerel fish and fish-based street food
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| title_full |
Detection and characterization of vibrio parahaemolyticus in raw mackerel fish and fish-based street food
|
| title_fullStr |
Detection and characterization of vibrio parahaemolyticus in raw mackerel fish and fish-based street food
|
| title_full_unstemmed |
Detection and characterization of vibrio parahaemolyticus in raw mackerel fish and fish-based street food
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| title_short |
Detection and characterization of vibrio parahaemolyticus in raw mackerel fish and fish-based street food
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| title_sort |
detection and characterization of vibrio parahaemolyticus in raw mackerel fish and fish-based street food
|