Benzyl- Isothiocyanates signaling in stomatal function of guard cells of Arabidopsis plants under biotic stress

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building INTELEK Repository
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collectionurl https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072
date 2017-05-24 15:05:37
eventvenue Primula Beach Hotel Kuala Terengganu, Terengganu
format Restricted Document
id 6115
institution UniSZA
originalfilename 0892-01-FH03-FBIM-18-13050.pdf
person 村田芳行
recordtype oai_dc
resourceurl https://intelek.unisza.edu.my/intelek/pages/view.php?ref=6115
spelling 6115 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=6115 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection407072 Restricted Document Conference Conference Paper application/pdf 7 1.6 Adobe Acrobat Pro DC 20 Paper Capture Plug-in 村田芳行 2017-05-24 15:05:37 0892-01-FH03-FBIM-18-13050.pdf UniSZA Private Access Benzyl- Isothiocyanates signaling in stomatal function of guard cells of Arabidopsis plants under biotic stress Stomatal opening provides access to inner leaf tissues for many plant pathogens, so narrowing stomatal apertures may be advantageous for plant defense. Benzyl-isothiocyanate (BITC) is generated from leaf epidermis cell walls of Arabidopsis plants during damages by pathogens or anyway. The role of BITC on guard cells of Arabidopsis was justified. BITC at 50 M or more induced stomatal closing which was partially inhibited by diphenylene iodonium (DPI), N G -nitro-L-Arg-methyl ester (L-NAME) and catalase. BITC-induced reactive oxygen species (ROS) in guard cells were significantly inhibited by DPI and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1- oxyl-3-oxide (cPTIO). BITC-induced ROS production was impaired in guard cells of atrbohD/F, respiratory burst oxidase homologues. BITC depleted glutathione (GSH) levels of guard cells to increase sensitivity of BITC to stomatal closure which was returned by the application of GSH. These results support that ROS and NO production and redox regulation in signaling of BITC to stomatal aperture to control insect attached. International Cell Signalling Conference 2017 Primula Beach Hotel Kuala Terengganu, Terengganu
spellingShingle Benzyl- Isothiocyanates signaling in stomatal function of guard cells of Arabidopsis plants under biotic stress
summary Stomatal opening provides access to inner leaf tissues for many plant pathogens, so narrowing stomatal apertures may be advantageous for plant defense. Benzyl-isothiocyanate (BITC) is generated from leaf epidermis cell walls of Arabidopsis plants during damages by pathogens or anyway. The role of BITC on guard cells of Arabidopsis was justified. BITC at 50 M or more induced stomatal closing which was partially inhibited by diphenylene iodonium (DPI), N G -nitro-L-Arg-methyl ester (L-NAME) and catalase. BITC-induced reactive oxygen species (ROS) in guard cells were significantly inhibited by DPI and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1- oxyl-3-oxide (cPTIO). BITC-induced ROS production was impaired in guard cells of atrbohD/F, respiratory burst oxidase homologues. BITC depleted glutathione (GSH) levels of guard cells to increase sensitivity of BITC to stomatal closure which was returned by the application of GSH. These results support that ROS and NO production and redox regulation in signaling of BITC to stomatal aperture to control insect attached.
title Benzyl- Isothiocyanates signaling in stomatal function of guard cells of Arabidopsis plants under biotic stress
title_full Benzyl- Isothiocyanates signaling in stomatal function of guard cells of Arabidopsis plants under biotic stress
title_fullStr Benzyl- Isothiocyanates signaling in stomatal function of guard cells of Arabidopsis plants under biotic stress
title_full_unstemmed Benzyl- Isothiocyanates signaling in stomatal function of guard cells of Arabidopsis plants under biotic stress
title_short Benzyl- Isothiocyanates signaling in stomatal function of guard cells of Arabidopsis plants under biotic stress
title_sort benzyl- isothiocyanates signaling in stomatal function of guard cells of arabidopsis plants under biotic stress