2026_Extraction Optimisation, Characterisation and In Vivo Pharmacokinetic Evaluation of Goniothalamin In Mice
| Format: | General Document |
|---|
| _version_ | 1860798362994868224 |
|---|---|
| building | INTELEK Repository |
| collection | Online Access |
| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection8797 |
| copyright | Copyright©PWB2026 |
| country | Malaysia |
| date | 2025-07-14 |
| format | General Document |
| id | 17469 |
| institution | UniSZA |
| originalfilename | 17469_50af1aa6993e9dc.pdf |
| person | Nor Hadirah Iskandar |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=17469 |
| sourcemedia | Server storage Scanned document |
| spelling | 17469 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=17469 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection8797 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.7 www.ilovepdf.com 160 Public Access Server storage Scanned document Universiti Sultan Zainal Abidin Universiti Sultan Zainal Abidin Dissertations, Academic High-Performance Liquid Chromatography (HPLC) Copyright©PWB2026 Thesis 2025-07-14 Nor Hadirah Iskandar Extraction Optimisation Goniothalamin Goniothalamus Andersonii Annonaceae Styrylpyrone Derivative High-performance Thin-layer Chromatography (HPTLC) Pharmacokinetics Liquid-Liquid Extraction (LLE) Nuclear Nagnetic Resonance (NMR) Gas Chromatography-Mass Spectrometry (GCMS) Bioavailability Annonaceae Plant Extracts Natural Products Pharmacokinetics Liquid Chromatography Nuclear Magnetic Resonance Spectroscopy 2026_Extraction Optimisation, Characterisation and In Vivo Pharmacokinetic Evaluation of Goniothalamin In Mice Goniothalamin is a styrylpyrone derivative which is commonly found in the genus Goniothalamus, part of the Annonaceae family, renowned for its abundance of bioactive secondary metabolites. It has attracted considerable interest for its notable biological activities, including cytotoxic, antiproliferative and antimicrobial effects. However, despite its pharmaceutical potential, challenges persist in achieving efficient extraction and accurate detection of goniothalamin to fully harness its benefits. Thus, the objectives of this study were to optimise the extraction and detection methods, to characterise the structure and to determine the in vivo pharmacokinetic profiles of the isolated goniothalamin. The powdered dried Goniothalamus andersonii stem bark was extracted separately in four solvents including hexane, petroleum ether, ethyl acetate and methanol, followed by drying under vacuo to collect the respective extracts. Goniothalamin was detected by high-performance thin-layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC), while characterisation was done using nuclear magnetic resonance (NMR) spectroscopy and gas chromatography-mass spectrometry (GCMS). A goniothalamin aliquot (40 mg/kg) was administered into male BALB/c mice via intraperitoneal (IP) injection. The serum samples were then prepared from the blood collected via the cardiac puncture technique at 10 different time points (n=50). Goniothalamin was extracted from the serum using liquid-liquid extraction (LLE) before being analysed using ultra-high-performance liquid chromatography coupled with a triple-quadrupole mass spectrometer (UHPLC-MS/MS-QQQ). In this study, the determined optimised HPLC method used ultrapure water (A) and methanol (B) with gradient elutions of 0–50% B (10 min), 50–100% B (30 min) and 100–60% B (10 min), operated at a flow rate of 1.0 mL/min. The goniothalamin signal was detected at min 24.1 with a limit of detection (LOD) and limit of quantification (LOQ) of 1.55 and 5.16 μg/mL, respectively. HPLC quantification indicated that the highest amount of goniothalamin was found in the hexane extract (41.6±3.2%) as compared to other extracts. The 1D-NMR spectrum showed the common signals of goniothalamin, while GCMS analysis revealed its molecular formula of C13H12O2 ([M]+ m/z 200.1). The pharmacokinetics study revealed that goniothalamin achieved a maximum serum concentration of 145.6 ± 81.6 ng/mL at min 5 and the half-life (t1/2) was 172.3 ± 61.9 min. This project developed optimised extraction and HPLC detection methods for goniothalamin from G. andersonii stem bark extracts, in addition to a rapid and sensitive method development and validation for assessing the bioavailability of goniothalamin in BALB/c mice employing UHPLC-MS/MS. This study is the first report on the pharmacokinetic profiles after goniothalamin exposure in BALB/c mice. These findings are important for future clinical research as the association between the bioavailability study and the pharmacological effect of this compound can be established. uuid:7fab88b6-8de9-4ea2-ad5b-6881d23cc8b6 17469_50af1aa6993e9dc.pdf |
| spellingShingle | 2026_Extraction Optimisation, Characterisation and In Vivo Pharmacokinetic Evaluation of Goniothalamin In Mice |
| state | Terengganu |
| subject | Dissertations, Academic Annonaceae Plant Extracts Natural Products Pharmacokinetics Liquid Chromatography Nuclear Magnetic Resonance Spectroscopy |
| summary | Goniothalamin is a styrylpyrone derivative which is commonly found in the genus Goniothalamus, part of the Annonaceae family, renowned for its abundance of bioactive secondary metabolites. It has attracted considerable interest for its notable biological activities, including cytotoxic, antiproliferative and antimicrobial effects. However, despite its pharmaceutical potential, challenges persist in achieving efficient extraction and accurate detection of goniothalamin to fully harness its benefits. Thus, the objectives of this study were to optimise the extraction and detection methods, to characterise the structure and to determine the in vivo pharmacokinetic profiles of the isolated goniothalamin. The powdered dried Goniothalamus andersonii stem bark was extracted separately in four solvents including hexane, petroleum ether, ethyl acetate and methanol, followed by drying under vacuo to collect the respective extracts. Goniothalamin was detected by high-performance thin-layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC), while characterisation was done using nuclear magnetic resonance (NMR) spectroscopy and gas chromatography-mass spectrometry (GCMS). A goniothalamin aliquot (40 mg/kg) was administered into male BALB/c mice via intraperitoneal (IP) injection. The serum samples were then prepared from the blood collected via the cardiac puncture technique at 10 different time points (n=50). Goniothalamin was extracted from the serum using liquid-liquid extraction (LLE) before being analysed using ultra-high-performance liquid chromatography coupled with a triple-quadrupole mass spectrometer (UHPLC-MS/MS-QQQ). In this study, the determined optimised HPLC method used ultrapure water (A) and methanol (B) with gradient elutions of 0–50% B (10 min), 50–100% B (30 min) and 100–60% B (10 min), operated at a flow rate of 1.0 mL/min. The goniothalamin signal was detected at min 24.1 with a limit of detection (LOD) and limit of quantification (LOQ) of 1.55 and 5.16 μg/mL, respectively. HPLC quantification indicated that the highest amount of goniothalamin was found in the hexane extract (41.6±3.2%) as compared to other extracts. The 1D-NMR spectrum showed the common signals of goniothalamin, while GCMS analysis revealed its molecular formula of C13H12O2 ([M]+ m/z 200.1). The pharmacokinetics study revealed that goniothalamin achieved a maximum serum concentration of 145.6 ± 81.6 ng/mL at min 5 and the half-life (t1/2) was 172.3 ± 61.9 min. This project developed optimised extraction and HPLC detection methods for goniothalamin from G. andersonii stem bark extracts, in addition to a rapid and sensitive method development and validation for assessing the bioavailability of goniothalamin in BALB/c mice employing UHPLC-MS/MS. This study is the first report on the pharmacokinetic profiles after goniothalamin exposure in BALB/c mice. These findings are important for future clinical research as the association between the bioavailability study and the pharmacological effect of this compound can be established. |
| title | 2026_Extraction Optimisation, Characterisation and In Vivo Pharmacokinetic Evaluation of Goniothalamin In Mice |
| title_full | 2026_Extraction Optimisation, Characterisation and In Vivo Pharmacokinetic Evaluation of Goniothalamin In Mice |
| title_fullStr | 2026_Extraction Optimisation, Characterisation and In Vivo Pharmacokinetic Evaluation of Goniothalamin In Mice |
| title_full_unstemmed | 2026_Extraction Optimisation, Characterisation and In Vivo Pharmacokinetic Evaluation of Goniothalamin In Mice |
| title_short | 2026_Extraction Optimisation, Characterisation and In Vivo Pharmacokinetic Evaluation of Goniothalamin In Mice |
| title_sort | 2026_extraction optimisation, characterisation and in vivo pharmacokinetic evaluation of goniothalamin in mice |