2024_Allelopathic Effect Of Chrysopogon Zizanioides (L.) Roberty On Physiological Responses And Metabolites Changes Of Target Plant Species
| Format: | General Document |
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2024-10-21 |
| format | General Document |
| id | 17334 |
| institution | UniSZA |
| originalfilename | ALLELOPATHIC EFFECT OF Chrysopogon zizanioides (L.) ROBERTY ON PHYSIOLOGICAL RESPONSES AND METABOLITES CHANGES OF TARGET PLANT SPECIES (PHD_2024).pdf |
| person | Muhd Arif Shaffiq Sahrir |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=17334 |
| sourcemedia | Server storage Scanned document |
| spelling | 17334 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=17334 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.7 Microsoft® Word 2019 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin Copyright©PWB2025 Allelopathy Allelopathy 221 Dissertations, Academic 2024-10-21 Allelochemicals Weeds—Control Muhd Arif Shaffiq Sahrir Chrysopogon zizanioides Vetiver grass extract Weed inhibition Metabolomic analysis GC-MS analysis Methanolic extract Physiological responses Carbohydrate metabolism Amino acid metabolism Pre-emergence treatment Post-emergence treatment Bioassays Bioherbicide potential Chrysopogon zizanioides Plant extracts—Analysis Herbicides—Biodegradation Metabolomics Gas chromatography–Mass spectrometry Plant metabolites Plant physiology–Biological pest control–Botanical pesticides 2024_Allelopathic Effect Of Chrysopogon Zizanioides (L.) Roberty On Physiological Responses And Metabolites Changes Of Target Plant Species Weeds detrimentally impact agriculture by reducing crop yield and increasing production cost of crop. While the allelopathic properties of C. zizanioides have been recognized, a comprehensive understanding of its effects and underlying mechanisms is lacking. This study addresses this gap by investigating the allelopathic potential of C. zizanioides on target plant species. The allelochemicals responsible for these effects were identified, and the mode of action was explored through metabolomic analysis. Additionally, the study examines the pre- and post-emergence effects of C. zizanioides on common weeds. Allelopathic activity of the leaf and root aqueous and methanolic extract (MeOH) of C. zizanioides was investigated through a petri dish bioassays experiment on Brassica rapa subsp. chinensis var. parachinensis, Triticum aestivum, Praxelis clematidea and Eleusine indica. The concentrations in the experiments were 0 (control), 0.1, 1.0, 10, 50 and 100 mg/mL of aqueous and methanolic extract (MeOH). The extracts were then analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) to identify the potential allelochemicals involved in the inhibition towards target species. Hence, the mode of action of the extract was investigated using B. rapa as a model plant in order to explore the metabolomic changes. Further, the pre- and post-emergence experiments were conducted in the greenhouse to examine the effect of the potent extract on the physiological, biochemical and metabolite changes of weeds. C. zizanioides root methanolic extract (MeOH) showed the highest inhibitory effect, as this extract at 50 and 100 mg/mL inhibited the root and shoot length of B. rapa, P. clematidea and E. indica up to 100% compared to the other extracts. The GC-MS analysis revealed that C. zizanioides root methanolic extract (MeOH) contained terpenoids such as valerenic acid, δ-cadinol and (E)-Eremophila-1(10),7(11)-dien-12-yl acetate. B. rapa showed a decrease in chlorophyll (chl a and b) content when 100 mg/mL of C. zizanioides methanolic root extract was applied. A total of 26 significant metabolites were detected by GC-MS (p<0.05) in B. rapa. Pre-emergence experiments showed that the C. zizanioides root methanolic extract (MeOH) reduced E. indica germination by 36.68% and P. clematidea by 63.33%. The post-emergence treatment of the extract significantly reduced the biomass and affected the shoot and root length of the tested weeds. There were also significant increments of proline, malondialdehyde and superoxide dismutase enzymes in weeds when exposed to 100 mg/mL extract. The metabolomic approach showed that the C. zizanioides root methanolic extract (MeOH) affected targeted weed species' carbohydrate and amino acid metabolism. This study found that C. zizanioides root extract inhibited target species in laboratory and greenhouse conditions. GC-MS analysis of the inhibitory root methanol extract (MeOH) from C. zizanioides revealed potential allelochemicals, including terpenoids, carboxylic acids, carbohydrates and amino acids. C. zizanioides root methanolic extract (MeOH) enhanced metabolism in plant abiotic stress regulators such as the propanoate pathway. Pre- and post-emergence C. zizanioides root extract treatment increased weed stress biomarkers and weed growth. The extract also affected target weed carbohydrate and amino acid metabolism. These findings provide insight into the allelopathic effect of C. zizanioides and contribute to the development of bioherbicides. uuid:AFE9CF54-8657-4478-B261-08128CBA523C ALLELOPATHIC EFFECT OF Chrysopogon zizanioides (L.) ROBERTY ON PHYSIOLOGICAL RESPONSES AND METABOLITES CHANGES OF TARGET PLANT SPECIES (PHD_2024).pdf Thesis |
| spellingShingle | 2024_Allelopathic Effect Of Chrysopogon Zizanioides (L.) Roberty On Physiological Responses And Metabolites Changes Of Target Plant Species |
| state | Terengganu |
| subject | Allelopathy Dissertations, Academic Weeds—Control Chrysopogon zizanioides Plant extracts—Analysis Herbicides—Biodegradation Metabolomics Gas chromatography–Mass spectrometry Plant metabolites Plant physiology–Biological pest control–Botanical pesticides |
| summary | Weeds detrimentally impact agriculture by reducing crop yield and increasing production cost of crop. While the allelopathic properties of C. zizanioides have been recognized, a comprehensive understanding of its effects and underlying mechanisms is lacking. This study addresses this gap by investigating the allelopathic potential of C. zizanioides on target plant species. The allelochemicals responsible for these effects were identified, and the mode of action was explored through metabolomic analysis. Additionally, the study examines the pre- and post-emergence effects of C. zizanioides on common weeds. Allelopathic activity of the leaf and root aqueous and methanolic extract (MeOH) of C. zizanioides was investigated through a petri dish bioassays experiment on Brassica rapa subsp. chinensis var. parachinensis, Triticum aestivum, Praxelis clematidea and Eleusine indica. The concentrations in the experiments were 0 (control), 0.1, 1.0, 10, 50 and 100 mg/mL of aqueous and methanolic extract (MeOH). The extracts were then analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) to identify the potential allelochemicals involved in the inhibition towards target species. Hence, the mode of action of the extract was investigated using B. rapa as a model plant in order to explore the metabolomic changes. Further, the pre- and post-emergence experiments were conducted in the greenhouse to examine the effect of the potent extract on the physiological, biochemical and metabolite changes of weeds. C. zizanioides root methanolic extract (MeOH) showed the highest inhibitory effect, as this extract at 50 and 100 mg/mL inhibited the root and shoot length of B. rapa, P. clematidea and E. indica up to 100% compared to the other extracts. The GC-MS analysis revealed that C. zizanioides root methanolic extract (MeOH) contained terpenoids such as valerenic acid, δ-cadinol and (E)-Eremophila-1(10),7(11)-dien-12-yl acetate. B. rapa showed a decrease in chlorophyll (chl a and b) content when 100 mg/mL of C. zizanioides methanolic root extract was applied. A total of 26 significant metabolites were detected by GC-MS (p<0.05) in B. rapa. Pre-emergence experiments showed that the C. zizanioides root methanolic extract (MeOH) reduced E. indica germination by 36.68% and P. clematidea by 63.33%. The post-emergence treatment of the extract significantly reduced the biomass and affected the shoot and root length of the tested weeds. There were also significant increments of proline, malondialdehyde and superoxide dismutase enzymes in weeds when exposed to 100 mg/mL extract. The metabolomic approach showed that the C. zizanioides root methanolic extract (MeOH) affected targeted weed species' carbohydrate and amino acid metabolism. This study found that C. zizanioides root extract inhibited target species in laboratory and greenhouse conditions. GC-MS analysis of the inhibitory root methanol extract (MeOH) from C. zizanioides revealed potential allelochemicals, including terpenoids, carboxylic acids, carbohydrates and amino acids. C. zizanioides root methanolic extract (MeOH) enhanced metabolism in plant abiotic stress regulators such as the propanoate pathway. Pre- and post-emergence C. zizanioides root extract treatment increased weed stress biomarkers and weed growth. The extract also affected target weed carbohydrate and amino acid metabolism. These findings provide insight into the allelopathic effect of C. zizanioides and contribute to the development of bioherbicides. |
| title | 2024_Allelopathic Effect Of Chrysopogon Zizanioides (L.) Roberty On Physiological Responses And Metabolites Changes Of Target Plant Species |
| title_full | 2024_Allelopathic Effect Of Chrysopogon Zizanioides (L.) Roberty On Physiological Responses And Metabolites Changes Of Target Plant Species |
| title_fullStr | 2024_Allelopathic Effect Of Chrysopogon Zizanioides (L.) Roberty On Physiological Responses And Metabolites Changes Of Target Plant Species |
| title_full_unstemmed | 2024_Allelopathic Effect Of Chrysopogon Zizanioides (L.) Roberty On Physiological Responses And Metabolites Changes Of Target Plant Species |
| title_short | 2024_Allelopathic Effect Of Chrysopogon Zizanioides (L.) Roberty On Physiological Responses And Metabolites Changes Of Target Plant Species |
| title_sort | 2024_allelopathic effect of chrysopogon zizanioides (l.) roberty on physiological responses and metabolites changes of target plant species |