2024_Genetic Diversity And F1 Hybrid Purity Testing Of Waxy Corn Using Simple Sequence Repeat Markers
| Format: | General Document |
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| collectionurl | https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 |
| copyright | Copyright©PWB2025 |
| country | Malaysia |
| date | 2024-10-20 |
| format | General Document |
| id | 17332 |
| institution | UniSZA |
| originalfilename | 17332_d737bb73ec76d6d.pdf |
| person | Nurul Fatini Saiful Lazim |
| recordtype | oai_dc |
| resourceurl | https://intelek.unisza.edu.my/intelek/pages/view.php?ref=17332 |
| sourcemedia | Server storage Scanned document |
| spelling | 17332 https://intelek.unisza.edu.my/intelek/pages/view.php?ref=17332 https://intelek.unisza.edu.my/intelek/pages/search.php?search=!collection3 General Document Malaysia Library Staff (Top Management) Library Staff (Management) Library Staff (Support) Terengganu Faculty of Bio-resources & Food Industry English application/pdf 1.7 93 Server storage Scanned document Universiti Sultan Zainal Abidin UniSZA Private Access Universiti Sultan Zainal Abidin Copyright©PWB2025 Dissertations, Academic Microsoft® Word 2021 2024-10-20 Nurul Fatini Saiful Lazim Waxy corn Zea mays L. var. ceratina Simple sequence repeat (SSR) markers Marker-assisted selection (MAS) Genetic diversity Inbred lines F1 hybrid Hybrid purity testing PCR-SSR Dendrogram Cluster analysis Heterosis Breeding Genomic DNA Plant breeding efficiency Zea mays — Genetic variation Waxy corn — Genetics Corn — Inbred lines Hybrid corn — Purity testing Molecular markers (Genetics) — SSR Plant breeding — Molecular techniques Heterosis — Corn DNA analysis — Plants Genetic diversity — Corn Agricultural biotechnology — Malaysia 2024_Genetic Diversity And F1 Hybrid Purity Testing Of Waxy Corn Using Simple Sequence Repeat Markers Waxy corn (Zea mays L. var. ceratina) is a type of corn with high anthocyanins content that can be used as an antioxidant source to improve human health. However, it has a low yield capacity for commercialization. Morphological evaluation of seeds from the grow-out test is mandatory in Malaysia, but it is time-consuming and costly. Therefore, marker-assisted selection (MAS) and breeding of waxy corn for high yield is promising to increase its production. The objectives of this research were to screen simple sequence repeat (SSR) markers, determine the genetic variation of waxy corn inbred lines, and use SSR markers to test the purity of filial 1 (F1) hybrids derived from inbred lines. Genomic DNA from eight waxy corn and four grain corn inbred lines was extracted from seeds and leaves using DNeasy Plant Mini Kit. In this study, 39 pairs of SSR primers were screened and the polymerase chain reaction – simple sequence repeat (PCR-SSR) products were detected using a gel documentation system. Next, dendrogram was constructed based on unweighted pair group method with arithmetic mean (UPGMA) using phylogenetic analysis using parsimony (PAUP) version 4.0 software. To check the purity of F1 hybrids, genomic DNA of 20 waxy corn hybrids along with their inbred lines, and three commercial hybrids was extracted from seeds. The PCR-SSR amplification was performed and the complementary banding pattern between the hybrids and the corresponding inbred lines was observed. Out of the 39 SSR primers screened, only ten primers (bnlg2162, umc1153, umc2366, phi112, bnlg2181, umc2077, bnlg381, umc1005, umc1196 and phi011) produced the clearest and reproducible bands compared to others. The ten primers were highly polymorphic with 100% polymorphisms and produced a total of 31 bands. The dendrogram produced two major clusters, cluster I and cluster II. Cluster I consisted of all waxy corn accessions (except BELLA 1-5) and two grain corn accessions GWT 10-17 and GWT 10-51. Cluster II consisted of waxy corn BELLA 1-5 and two grain corn accessions GWT 46-1b and GWT 46-10b. Among the waxy corn and grain corn accessions, BELLA 1-10 and GWT 46-1b showed the greatest distance with the value of 0.58. From the ten primers, only one primer (bnlg381) produced complementary banding pattern between both inbred lines, which made a way to identify the hybrid. The hybrid BELLA 1-8 x BELLA 1-7 has both bands from its parents at 300 bp and 200 bp, confirming the genetic purity of this hybrid seed. Identification of genetically distant inbred lines using SSR markers is essential for maximizing yield with a high probability of heterosis and used for breeding of new and improved waxy corn varieties. Moreover, the hybrid seed industry will benefit greatly from the SSR marker identified in this study, which will enable a cheaper and more efficient way of selection and evaluation of waxy corn hybrid seeds. uuid:B6A56F6B-8F07-4BF5-92E2-F498BB3D6CDF 17332_d737bb73ec76d6d.pdf Thesis |
| spellingShingle | 2024_Genetic Diversity And F1 Hybrid Purity Testing Of Waxy Corn Using Simple Sequence Repeat Markers |
| state | Terengganu |
| subject | Dissertations, Academic Zea mays — Genetic variation Waxy corn — Genetics Corn — Inbred lines Hybrid corn — Purity testing Molecular markers (Genetics) — SSR Plant breeding — Molecular techniques Heterosis — Corn DNA analysis — Plants Genetic diversity — Corn Agricultural biotechnology — Malaysia |
| summary | Waxy corn (Zea mays L. var. ceratina) is a type of corn with high anthocyanins content that can be used as an antioxidant source to improve human health. However, it has a low yield capacity for commercialization. Morphological evaluation of seeds from the grow-out test is mandatory in Malaysia, but it is time-consuming and costly. Therefore, marker-assisted selection (MAS) and breeding of waxy corn for high yield is promising to increase its production. The objectives of this research were to screen simple sequence repeat (SSR) markers, determine the genetic variation of waxy corn inbred lines, and use SSR markers to test the purity of filial 1 (F1) hybrids derived from inbred lines. Genomic DNA from eight waxy corn and four grain corn inbred lines was extracted from seeds and leaves using DNeasy Plant Mini Kit. In this study, 39 pairs of SSR primers were screened and the polymerase chain reaction – simple sequence repeat (PCR-SSR) products were detected using a gel documentation system. Next, dendrogram was constructed based on unweighted pair group method with arithmetic mean (UPGMA) using phylogenetic analysis using parsimony (PAUP) version 4.0 software. To check the purity of F1 hybrids, genomic DNA of 20 waxy corn hybrids along with their inbred lines, and three commercial hybrids was extracted from seeds. The PCR-SSR amplification was performed and the complementary banding pattern between the hybrids and the corresponding inbred lines was observed. Out of the 39 SSR primers screened, only ten primers (bnlg2162, umc1153, umc2366, phi112, bnlg2181, umc2077, bnlg381, umc1005, umc1196 and phi011) produced the clearest and reproducible bands compared to others. The ten primers were highly polymorphic with 100% polymorphisms and produced a total of 31 bands. The dendrogram produced two major clusters, cluster I and cluster II. Cluster I consisted of all waxy corn accessions (except BELLA 1-5) and two grain corn accessions GWT 10-17 and GWT 10-51. Cluster II consisted of waxy corn BELLA 1-5 and two grain corn accessions GWT 46-1b and GWT 46-10b. Among the waxy corn and grain corn accessions, BELLA 1-10 and GWT 46-1b showed the greatest distance with the value of 0.58. From the ten primers, only one primer (bnlg381) produced complementary banding pattern between both inbred lines, which made a way to identify the hybrid. The hybrid BELLA 1-8 x BELLA 1-7 has both bands from its parents at 300 bp and 200 bp, confirming the genetic purity of this hybrid seed. Identification of genetically distant inbred lines using SSR markers is essential for maximizing yield with a high probability of heterosis and used for breeding of new and improved waxy corn varieties. Moreover, the hybrid seed industry will benefit greatly from the SSR marker identified in this study, which will enable a cheaper and more efficient way of selection and evaluation of waxy corn hybrid seeds. |
| title | 2024_Genetic Diversity And F1 Hybrid Purity Testing Of Waxy Corn Using Simple Sequence Repeat Markers |
| title_full | 2024_Genetic Diversity And F1 Hybrid Purity Testing Of Waxy Corn Using Simple Sequence Repeat Markers |
| title_fullStr | 2024_Genetic Diversity And F1 Hybrid Purity Testing Of Waxy Corn Using Simple Sequence Repeat Markers |
| title_full_unstemmed | 2024_Genetic Diversity And F1 Hybrid Purity Testing Of Waxy Corn Using Simple Sequence Repeat Markers |
| title_short | 2024_Genetic Diversity And F1 Hybrid Purity Testing Of Waxy Corn Using Simple Sequence Repeat Markers |
| title_sort | 2024_genetic diversity and f1 hybrid purity testing of waxy corn using simple sequence repeat markers |